Q65DS9 (SYR_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	BLi03971, BL03923

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus licheniformis

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00123
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00123.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Arginine--tRNA ligase HAMAP-Rule MF_00123		PRO_0000241983
Regions
	Motif	132 – 142	11	"HIGH" region HAMAP-Rule MF_00123

Sequences

	Sequence		Length	Mass (Da)
	Q65DS9 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 40FE575CDC203DAC	FASTA	556	62,656
	10 20 30 40 50 60 MNIVEQMKDV LKQEIKEAVM KAGLAEESEI PEVVLEVPKD KSHGDYSTNM AMQLARIAKK 70 80 90 100 110 120 APRNIAEEIV ASFDKGKASI DKLDIAGPGF INFYMNNQYL TKLIPAVLEA GEAYGETNIG 130 140 150 160 170 180 GGERVQVEFV SANPTGNLHL GHARGAAVGD SLCNVLEKAG YEVSREYYIN DAGNQINNLA 190 200 210 220 230 240 LSVEARYFQA LGKDKPMPED GYHGEDIKEI GQKLADEFGD RFVHEEESER LAFFREYGLK 250 260 270 280 290 300 YELGKLREDL ENFRVPFDVW YSETSLYQNG KIDQALEALR EKGHIYEEDG ATWFRSTAFG 310 320 330 340 350 360 DDKDRVLIKK DGSYTYLLPD IAYHKDKLDR GFQKLINVWG ADHHGYIPRM KAAIEALGYD 370 380 390 400 410 420 KETLEVEIIQ LVHLYKNGEK MKMSKRTGKA VTMRDLIDEV GLDAVRYFFA MRSADTHMDF 430 440 450 460 470 480 DLDLAVSTSN ENPVYYAQYA HARICSMLRQ GEEKGISFEG NLDLTKIGSE KEYDLLKVIG 490 500 510 520 530 540 SFPEAVADAA EKRIPHRITN YIFELASVLH SFYNAEKVLD PADEEKSRAR LSLMKATQIT 550 LNNALKLIGV SAPEKM

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU42785.1. CP000002 Genomic DNA. Translation: AAU25411.1.
RefSeq	YP_006715249.1. NC_006322.1. YP_081049.1. NC_006270.3.
3D structure databases
ProteinModelPortal	Q65DS9.
ModBase	Search...
Protein-protein interaction databases
STRING	279010.BL03923.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU25411; AAU25411; BL03923. AAU42785; AAU42785; BLi03971.
GeneID	3027964. 3101407.
KEGG	bld:BLi03971. bli:BL03923.
PATRIC	18953740. VBIBacLic203714_4045.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0018.
HOGENOM	HOG000247214.
KO	K01887.
OMA	HTSANPN.
ProtClustDB	PRK01611.
Enzyme and pathway databases
BioCyc	BLIC279010:GJ2P-3925-MONOMER.
Family and domain databases
Gene3D	3.30.1360.70. 1 hit. 3.40.50.620. 1 hit.
HAMAP	MF_00123. Arg_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_BACLD

Accession

Primary (citable) accession number: Q65DS9
Secondary accession number(s): Q62PA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	October 25, 2004
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program