ID   THIM_BACLD              Reviewed;         269 AA.
AC   Q65DJ9; Q62P19;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   16-JUN-2009, entry version 35.
DE   RecName: Full=Hydroxyethylthiazole kinase;
DE            EC=2.7.1.50;
DE   AltName: Full=4-methyl-5-beta-hydroxyethylthiazole kinase;
DE            Short=Thz kinase;
DE            Short=TH kinase;
GN   Name=thiM; OrderedLocusNames=BLi04052, BL03865;
OS   Bacillus licheniformis (strain DSM 13 / ATCC 14580).
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=279010;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15383718; DOI=10.1159/000079829;
RA   Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H.,
RA   Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R.,
RA   Ehrenreich A., Gottschalk G.;
RT   "The complete genome sequence of Bacillus licheniformis DSM13, an
RT   organism with great industrial potential.";
RL   J. Mol. Microbiol. Biotechnol. 7:204-211(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15461803; DOI=10.1186/gb-2004-5-10-r77;
RA   Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J.,
RA   Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G.,
RA   Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L.,
RA   Larsen T.S., Sorokin A., Bolotin A., Lapidus A., Galleron N.,
RA   Ehrlich S.D., Berka R.M.;
RT   "Complete genome sequence of the industrial bacterium Bacillus
RT   licheniformis and comparisons with closely related Bacillus species.";
RL   Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004).
CC   -!- CATALYTIC ACTIVITY: ATP + 4-methyl-5-(2-hydroxyethyl)thiazole =
CC       ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.
CC   -!- PATHWAY: Cofactor biosynthesis; thiamine pyrophosphate
CC       biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-
CC       5-(2-hydroxyethyl)-thiazole: step 1/1.
CC   -!- SIMILARITY: Belongs to the Thz kinase family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CP000002; AAU25492.1; -; Genomic_DNA.
DR   EMBL; AE017333; AAU42865.1; -; Genomic_DNA.
DR   RefSeq; YP_081130.1; -.
DR   RefSeq; YP_093558.1; -.
DR   GeneID; 3030181; -.
DR   GeneID; 3099156; -.
DR   GenomeReviews; AE017333_GR; BLi04052.
DR   GenomeReviews; CP000002_GR; BL03865.
DR   KEGG; bld:BLi04052; -.
DR   KEGG; bli:BL03865; -.
DR   NMPDR; fig|279010.5.peg.3173; -.
DR   HOGENOM; Q65DJ9; -.
DR   OMA; Q65DJ9; ASPVMAH.
DR   BioCyc; BLIC279010:BL03865-MON; -.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0004417; F:hydroxyethylthiazole kinase activity; IEA:HAMAP.
DR   GO; GO:0009228; P:thiamin biosynthetic process; IEA:UniProtKB-KW.
DR   HAMAP; MF_00228; -; 1.
DR   InterPro; IPR000417; Hyethyz_kinase.
DR   InterPro; IPR011144; Hyethyz_kinsmonf.
DR   PANTHER; PTHR20857:SF14; Hyethyz_kinase; 1.
DR   Pfam; PF02110; HK; 1.
DR   PIRSF; PIRSF000513; Thz_kinase; 1.
DR   PRINTS; PR01099; HYETHTZKNASE.
DR   TIGRFAMs; TIGR00694; thiM; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Kinase; Nucleotide-binding;
KW   Thiamine biosynthesis; Transferase.
FT   CHAIN         1    269       Hydroxyethylthiazole kinase.
FT                                /FTId=PRO_1000021501.
FT   BINDING      45     45       Substrate; via amide nitrogen (By
FT                                similarity).
FT   BINDING     121    121       ATP (By similarity).
FT   BINDING     167    167       ATP (By similarity).
FT   BINDING     194    194       Substrate; via amide nitrogen (By
FT                                similarity).
SQ   SEQUENCE   269 AA;  28240 MW;  DB464AB32443BCE7 CRC64;
     MNMEDAARGI ELVRKQKPLV HNMTNNVVTN FTANGLLALG ASPVMAYARE EAADMAKIAG
     ALVLNIGTLS RESVEAMIIA GKSANEHGVP VIFDPVGAGA TPFRTESAQA IMRKVKVSAV
     RGNAAEIANT LGEDWLIKGV DAGEESGDRT ELAKKAAKLW DTAVIITGAE DVITDGTKTY
     TVGNGHQLLT KVTGTGCLFT SVIGAFCAVE KDVCRAAVSA AAFYGTAAEL AAAKTESRGP
     GSFQIEFLNQ LAAVEAMDIK ERGRIREVE
//