Reviewed, UniProtKB/Swiss-Prot Q65DJ9 (THIM_BACLD)

Names and origin

Protein names

Recommended name:
    Hydroxyethylthiazole kinase
    EC=2.7.1.50
Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase
      Short name=Thz kinase
      Short name=TH kinase

Gene names

Name:	thiM
Ordered Locus Names:	BLi04052, BL03865

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Pathway	Cofactor biosynthesis; thiamine pyrophosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-5-(2-hydroxyethyl)-thiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_1000021501
Sites
	Binding site	45	1	Substrate; via amide nitrogen By similarity
	Binding site	121	1	ATP By similarity
	Binding site	167	1	ATP By similarity
	Binding site	194	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65DJ9-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: DB464AB32443BCE7	FASTA	269	28,240
	10 20 30 40 50 60 MNMEDAARGI ELVRKQKPLV HNMTNNVVTN FTANGLLALG ASPVMAYARE EAADMAKIAG 70 80 90 100 110 120 ALVLNIGTLS RESVEAMIIA GKSANEHGVP VIFDPVGAGA TPFRTESAQA IMRKVKVSAV 130 140 150 160 170 180 RGNAAEIANT LGEDWLIKGV DAGEESGDRT ELAKKAAKLW DTAVIITGAE DVITDGTKTY 190 200 210 220 230 240 TVGNGHQLLT KVTGTGCLFT SVIGAFCAVE KDVCRAAVSA AAFYGTAAEL AAAKTESRGP 250 260 GSFQIEFLNQ LAAVEAMDIK ERGRIREVE

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000002 Genomic DNA. Translation: AAU25492.1. AE017333 Genomic DNA. Translation: AAU42865.1.
RefSeq	YP_081130.1. YP_093558.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3030181. 3099156.
GenomeReviews	Gene locus BLi04052 in contig AE017333_GR. Gene locus BL03865 in contig CP000002_GR.
KEGG	bld:BLi04052. bli:BL03865.
NMPDR	fig|279010.5.peg.3173.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65DJ9.
OMA	Q65DJ9. ASPVMAH.
Enzyme and pathway databases
BioCyc	BLIC279010:BL03865-MON.
Family and domain databases
HAMAP	MF_00228. [Tree]
InterPro	IPR000417. Hyethyz_kinase. IPR011144. Hyethyz_kinsmonf. [Graphical view]
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. thiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_BACLD

Accession

Primary (citable) accession number: Q65DJ9
Secondary accession number(s): Q62P19

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2004
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)