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Protein

D-alanine--D-alanyl carrier protein ligase

Gene

dltA

Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.UniRule annotation

Catalytic activityi

D-alanine + ATP + holo-[D-alanyl-carrier protein] = AMP + diphosphate + D-alanyl-[D-alanyl-carrier protein].UniRule annotation

Pathwayi: lipoteichoic acid biosynthesis

This protein is involved in the pathway lipoteichoic acid biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway lipoteichoic acid biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei195D-alanineUniRule annotation1
Binding sitei299D-alanine; via carbonyl oxygenUniRule annotation1
Binding sitei381ATPUniRule annotation1
Binding sitei490ATPUniRule annotation1
Binding sitei490D-alanineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi150 – 151ATPUniRule annotation2
Nucleotide bindingi290 – 295ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBLIC279010:G1G12-4098-MONOMER
UniPathwayiUPA00556

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanyl carrier protein ligaseUniRule annotation (EC:6.2.1.-UniRule annotation)
Short name:
DCLUniRule annotation
Alternative name(s):
D-alanine--poly(phosphoribitol) ligase subunit 1UniRule annotation
D-alanine-activating enzymeUniRule annotation
Short name:
DAEUniRule annotation
Gene namesi
Name:dltAUniRule annotation
Ordered Locus Names:BLi04080, BL03934
OrganismiBacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000000606 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000255291 – 502D-alanine--D-alanyl carrier protein ligaseAdd BLAST502

Proteomic databases

PRIDEiQ65DH1

Interactioni

Protein-protein interaction databases

STRINGi279010.BLi04080

Structurei

3D structure databases

ProteinModelPortaliQ65DH1
SMRiQ65DH1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108IQD Bacteria
COG1020 LUCA
HOGENOMiHOG000229995
KOiK03367
OMAiNFYIIFT

Family and domain databases

HAMAPiMF_00593 DltA, 1 hit
InterProiView protein in InterPro
IPR010071 AA_adenyl_domain
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR010072 DltA
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
TIGRFAMsiTIGR01733 AA-adenyl-dom, 1 hit
TIGR01734 D-ala-DACP-lig, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

Q65DH1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLIETIKKY AQTQPDTLAF VNEEEKLTYG ELWSQSERLA ARIQSEALTD
60 70 80 90 100
ASPIIVYGHM KPVMAVSFLA CVKAGHPYIP VDVSIPADRI LKIINSSKAE
110 120 130 140 150
LLLNNSGTSV DTGDALISVV EPGVLEGDGV PETDPGRWVH GEDTFYIIYT
160 170 180 190 200
SGSTGNPKGV QISADNLQSF TDWITNDFPV ESGQVFLNQA PFSFDLSVMD
210 220 230 240 250
LYPCLQSGGT LWTVTKDMIN RPKLLFEALK QSNVNVWTST PSFAQMCLMD
260 270 280 290 300
PSYSEELLPE LSLFMFCGET LPASVARQLK ERFPKARVFN TYGPTEATVA
310 320 330 340 350
VTSIEVTDDV LNKYSSLPVG SEKPETEIVI INEDGKAVQD GEKGEIIITG
360 370 380 390 400
ASVSKGYLGE KALTEKAFFS YNGSPAYRTG DAGYKENGQL FFLGRLDFQI
410 420 430 440 450
KLHGYRIELE EIEYQINQSR YVQSAVVIPF YREEKIEYLI AMIVPAEHDF
460 470 480 490 500
EKEYQLTSAI KKDLGSKLPA YMIPRKFMYQ KEIPMTANGK IDRKRLKEEV

TV
Length:502
Mass (Da):56,010
Last modified:October 25, 2004 - v1
Checksum:iFCBF40EA617BC2B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000002 Genomic DNA Translation: AAU25520.1
AE017333 Genomic DNA Translation: AAU42893.1
RefSeqiWP_003186280.1, NC_006322.1

Genome annotation databases

EnsemblBacteriaiAAU25520; AAU25520; BL03934
AAU42893; AAU42893; BLi04080
GeneIDi3028795
KEGGibld:BLi04080
bli:BL03934
PATRICifig|279010.13.peg.4158

Similar proteinsi

Entry informationi

Entry nameiDLTA_BACLD
AccessioniPrimary (citable) accession number: Q65DH1
Secondary accession number(s): Q62NZ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 25, 2004
Last modified: May 23, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health