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Q65DC2 (PTLY_BACLD) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pectate trisaccharide-lyase
EC=4.2.2.22
Alternative name(s):
Exopolygalacturonate lyase
Pectate lyase
Gene names
Ordered Locus Names:BLi04129, BL00947
OrganismBacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]
Taxonomic identifier279010 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cleaves unsaturated oligo-galacturonides from pectin. The major product is trigalacturonate; digalacturonate and tetragalacturonate are also produced. Activity on methylated pectins decreases with an increasing degree of methylation By similarity. UniProtKB Q8GCB2 UniProtKB B1B6T1

Catalytic activity

Eliminative cleavage of unsaturated trigalacturonate as the major product from the reducing end of polygalacturonic acid/pectate. UniProtKB Q8GCB2

Cofactor

Calcium By similarity. UniProtKB Q8GCB2

Subcellular location

Secreted Probable.

Sequence similarities

Belongs to the polysaccharide lyase 1 family.

Contains 4 PbH1 repeats.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cell wall biogenesis/degradation
Polysaccharide degradation
   Cellular componentSecreted
   DomainRepeat
Signal
   LigandCalcium
Metal-binding
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcellular cell wall organization

Inferred from electronic annotation. Source: UniProtKB-KW

polysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionlyase activity

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Propeptide28 – 3912 By similarity UniProtKB Q8GCB2
PRO_0000405010
Chain40 – 341302Pectate trisaccharide-lyase UniProtKB Q8GCB2
PRO_0000405011

Regions

Repeat131 – 15626PbH1 1
Repeat158 – 18629PbH1 2
Repeat262 – 28322PbH1 3
Repeat287 – 32236PbH1 4

Sites

Active site2331 By similarity UniProtKB P39116
Metal binding1501Calcium By similarity UniProtKB P39116
Metal binding1801Calcium By similarity UniProtKB P39116
Metal binding1841Calcium By similarity UniProtKB P39116

Sequences

Sequence LengthMass (Da)Tools
Q65DC2 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 4662DFCBF915E9C6

FASTA34137,365
        10         20         30         40         50         60 
MKKLISIIFI FVLGVVGSLT AAVSAEAASA LNSGKVNPLA DFSLKGFAAL NGGTTGGEGG 

        70         80         90        100        110        120 
QTVTVTTGDQ LIAALKNKNA NTPLKIYVNG TITTSNTSAS KIDVKDVSNV SIVGSGTKGE 

       130        140        150        160        170        180 
LKGIGIKIWR ANNIIIRNLK IHEVASGDKD AIGIEGPSKN IWVDHNELYH SLNVDKDYYD 

       190        200        210        220        230        240 
GLFDVKRDAE YITFSWNYVH DGWKSMLMGS SDSDNYNRTI TFHHNWFENL NSRVPSFRFG 

       250        260        270        280        290        300 
EGHIYNNYFN KIIDSGINSR MGARIRIENN LFENAKDPIV SWYSSSPGYW HVSNNKFVNS 

       310        320        330        340 
RGSMPTTSTT TYNPPYSYSL DNVDNVKSIV KQNAGVGKIN P

« Hide

References

[1]"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species."
Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. expand/collapse author list , Galleron N., Ehrlich S.D., Berka R.M.
Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.
[2]"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000002 Genomic DNA. Translation: AAU25568.1.
AE017333 Genomic DNA. Translation: AAU42942.1.
RefSeqYP_081206.1. NC_006270.3.
YP_093635.1. NC_006322.1.

3D structure databases

ProteinModelPortalQ65DC2.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ65DC2.

Protein family/group databases

CAZyPL1. Polysaccharide Lyase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000055787; EBBACP00000054318; EBBACG00000055778.
EBBACT00000058514; EBBACP00000056947; EBBACG00000058505.
GeneID3028316.
3098687.
GenomeReviewsGene locus BLi04129 in contig AE017333_GR.
Gene locus BL00947 in contig CP000002_GR.
KEGGbld:BLi04129.
bli:BL00947.
NMPDRfig|279010.5.peg.3938.
PATRIC18954076. VBIBacLic203714_4213.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3866.
GeneTreeEBGT00050000000877.
HOGENOMHBG465545.
OMALFDVKRD.
ProtClustDBCLSK873712.

Enzyme and pathway databases

BioCycBLIC279010-1:BLI04129-MONOMER.
BLIC279010:BL00947-MONOMER.

Family and domain databases

InterProIPR002022. Amb_allergen.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
PfamPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
SMARTSM00656. Amb_all. 1 hit.
SM00710. PbH1. 4 hits.
[Graphical view]
SUPFAMSSF51126. Pectin_lyas_like. 1 hit.
ProtoNetSearch...

Entry information

Entry namePTLY_BACLD
AccessionPrimary (citable) accession number: Q65DC2
Secondary accession number(s): Q62NU3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: October 25, 2004
Last modified: January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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