Reviewed, UniProtKB/Swiss-Prot Q65D00 (IOLA2_BACLD)

Names and origin

Protein names

Recommended name:
    Methylmalonate semialdehyde dehydrogenase [acylating] 2
      Short name=MMSA dehydrogenase 2
      Short name=MMSDH 2
      Short name=MSDH 2
    EC=1.2.1.27
Alternative name(s):
    Malonate semialdehyde dehydrogenase [acetylating] 2
      Short name=MSA dehydrogenase 2
    EC=1.2.1.18

Gene names

Name:	iolA2
Ordered Locus Names:	BLi04251, BL00246

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively By similarity.
Catalytic activity	3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. HAMAP MF_01670 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. HAMAP MF_01670
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 7/7. HAMAP MF_01670
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. IolA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: HAMAP methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Methylmalonate semialdehyde dehydrogenase [acylating] 2 HAMAP MF_01670		PRO_0000352330
Regions
	Nucleotide binding	176 – 180	5	NAD By similarity
Sites
	Active site	284	1	Nucleophile By similarity
	Binding site	382	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65D00-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: B804B1EBB44F2FB7	FASTA	487	53,550
	10 20 30 40 50 60 MAEIRKLRNY INGEWVESKT DKYEDVVNPA TKEVLCQVPI STREDIEYAA QTAAEAFKTW 70 80 90 100 110 120 SKVAVPRRAR ILFNFQQLLT QHKEELAHLI TIENGKNTKE ALGEVGRGIE NVEFAAGAPS 130 140 150 160 170 180 LMMGDSLATI ATDVEAANYR YPIGVVGGIA PFNFPMMVPC WMFPMAIALG NTFILKPSER 190 200 210 220 230 240 TPLLTEKLVE LFEKAGLPKG VFNVVYGAHD VVNGILEHPE IKAISFVGSK PVGEYVYKKG 250 260 270 280 290 300 SENLKRVQSL TGAKNHTIVL NDANLEDTVT NIIGAAFGSA GERCMACAVV TVEEGIADEF 310 320 330 340 350 360 MEKLVQKASE VKIGNGLDDG VFLGPVIRED NKKRTLGYIQ KGIEEGARLV CDGRENVTDE 370 380 390 400 410 420 GYFVGPTIFD NVTTDMTIWK DEIFAPVLSV IRVKNLKEAV EIANQSEFAN GACLFTSNSN 430 440 450 460 470 480 AIRYFRENID AGMLGINLGV PAPMAFFPFS GWKSSFFGTL HANGKDSVDF YTRKKVVTAR YPAPDFN

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000002 Genomic DNA. Translation: AAU25685.1. AE017333 Genomic DNA. Translation: AAU43064.1.
RefSeq	YP_081323.1. YP_093757.1.
3D structure databases
SMR	Q65D00. Positions 3-486.
ModBase	Search...
Genome annotation databases
GeneID	3030827. 3098469.
GenomeReviews	Gene locus BLi04251 in contig AE017333_GR. Gene locus BL00246 in contig CP000002_GR.
KEGG	bld:BLi04251. bli:BL00246.
NMPDR	fig|279010.5.peg.4109.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65D00.
OMA	Q65D00. DARAWIP.
Family and domain databases
HAMAP	MF_01670. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLA2_BACLD

Accession

Primary (citable) accession number: Q65D00
Secondary accession number(s): Q62NH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	October 25, 2004
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)