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Q65CX5

- GANA_BACLD

UniProt

Q65CX5 - GANA_BACLD

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Protein

Arabinogalactan endo-beta-1,4-galactanase

Gene
ganB, galA, yvfO, BLi04276, BL00263
Organism
Bacillus licheniformis (strain DSM 13 / ATCC 14580)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes the beta-1,4-galactan linkages of arabinogalactan type I, a pectic substance found in plants such as soybeans.

Catalytic activityi

The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.

Cofactori

Binds 1 calcium ion per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei190 – 1901Proton donor Inferred
Binding sitei263 – 2631Substrate
Active sitei288 – 2881Nucleophile Inferred
Binding sitei292 – 2921Substrate
Metal bindingi299 – 2991Calcium
Metal bindingi301 – 3011Calcium
Metal bindingi303 – 3031Calcium
Binding sitei307 – 3071Substrate
Binding sitei384 – 3841Substrate
Metal bindingi392 – 3921Calcium
Metal bindingi395 – 3951Calcium

GO - Molecular functioni

  1. arabinogalactan endo-1,4-beta-galactosidase activity Source: UniProtKB-EC
  2. glucosidase activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-4230-MONOMER.

Protein family/group databases

CAZyiGH53. Glycoside Hydrolase Family 53.

Names & Taxonomyi

Protein namesi
Recommended name:
Arabinogalactan endo-beta-1,4-galactanase (EC:3.2.1.89)
Alternative name(s):
Endo-1,4-beta-galactanase
Short name:
Galactanase
Gene namesi
Name:ganB
Synonyms:galA, yvfO
Ordered Locus Names:BLi04276, BL00263
OrganismiBacillus licheniformis (strain DSM 13 / ATCC 14580)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000000606: Chromosome, UP000000608: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626 Reviewed predictionAdd
BLAST
Chaini27 – 424398Arabinogalactan endo-beta-1,4-galactanasePRO_0000371562Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi279010.BL00263.

Structurei

Secondary structure

1
424
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi50 – 545
Helixi58 – 636
Beta strandi73 – 753
Helixi78 – 847
Beta strandi89 – 946
Helixi113 – 12513
Beta strandi129 – 1346
Beta strandi136 – 1394
Beta strandi142 – 1443
Helixi150 – 1523
Helixi157 – 17721
Beta strandi182 – 1909
Helixi201 – 21818
Beta strandi222 – 2287
Helixi236 – 24611
Beta strandi252 – 2587
Turni260 – 2623
Helixi266 – 28015
Beta strandi283 – 2897
Beta strandi298 – 3014
Beta strandi304 – 3063
Helixi318 – 33316
Beta strandi339 – 3457
Helixi355 – 3573
Helixi358 – 36811
Beta strandi371 – 3733
Helixi375 – 3773
Turni378 – 3803
Turni382 – 3843
Helixi385 – 3884
Helixi395 – 3973
Beta strandi398 – 4003
Helixi408 – 4114
Helixi412 – 4187

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R8LX-ray2.60A/B26-424[»]
1UR0X-ray2.50A/B26-424[»]
1UR4X-ray2.20A/B26-424[»]
2CCRX-ray2.30A/B26-424[»]
2GFTX-ray2.30A/B26-424[»]
2J74X-ray2.60A/B28-424[»]
ProteinModelPortaliQ65CX5.
SMRiQ65CX5. Positions 36-422.

Miscellaneous databases

EvolutionaryTraceiQ65CX5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni142 – 1454Substrate binding
Regioni229 – 2302Substrate binding

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3867.
HOGENOMiHOG000118034.
KOiK01224.
OMAiIRLRLWV.
OrthoDBiEOG6H4K5M.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR011683. Glyco_hydro_53.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF07745. Glyco_hydro_53. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q65CX5-1 [UniParc]FASTAAdd to Basket

« Hide

MKNVLAVFVV LIFVLGAFGT SGPAEAARDS GTAKSGLYVE KVSGLRKDFI    50
KGVDVSSIIA LEESGVAFYN ESGKKQDIFK TLKEAGVNYV RVRIWNDPYD 100
ANGNGYGGGN NDLEKAIQIG KRATANGMKL LADFHYSDFW ADPAKQKAPK 150
AWANLNFEDK KTALYQYTKQ SLKAMKAAGI DIGMVQVGNE TNGGLAGETD 200
WAKMSQLFNA GSQAVRETDS NILVALHFTN PETSGRYAWI AETLHRHHVD 250
YDVFASSYYP FWHGTLKNLT SVLTSVADTY GKKVMVAETS YTYTAEDGDG 300
HGNTAPKNGQ TLNNPVTVQG QANAVRDVIQ AVSDVGEAGI GVFYWEPAWI 350
PVGPAHRLEK NKALWETYGS GWATSYAAEY DPEDAGKWFG GSAVDNQALF 400
DFKGRPLPSL HVFQYVDTGT PFKN 424
Length:424
Mass (Da):46,225
Last modified:October 25, 2004 - v1
Checksum:iE6CC3D0D1B80D166
GO

Sequence cautioni

The sequence AAU25711.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017333 Genomic DNA. Translation: AAU43089.1.
CP000002 Genomic DNA. Translation: AAU25711.1. Different initiation.
RefSeqiWP_011198463.1. NC_006270.3.
YP_006715543.1. NC_006322.1.
YP_081349.1. NC_006270.3.

Genome annotation databases

EnsemblBacteriaiAAU25711; AAU25711; BL00263.
AAU43089; AAU43089; BLi04276.
GeneIDi3029947.
3101427.
KEGGibld:BLi04276.
bli:BL00263.
PATRICi18954382. VBIBacLic203714_4366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017333 Genomic DNA. Translation: AAU43089.1 .
CP000002 Genomic DNA. Translation: AAU25711.1 . Different initiation.
RefSeqi WP_011198463.1. NC_006270.3.
YP_006715543.1. NC_006322.1.
YP_081349.1. NC_006270.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1R8L X-ray 2.60 A/B 26-424 [» ]
1UR0 X-ray 2.50 A/B 26-424 [» ]
1UR4 X-ray 2.20 A/B 26-424 [» ]
2CCR X-ray 2.30 A/B 26-424 [» ]
2GFT X-ray 2.30 A/B 26-424 [» ]
2J74 X-ray 2.60 A/B 28-424 [» ]
ProteinModelPortali Q65CX5.
SMRi Q65CX5. Positions 36-422.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 279010.BL00263.

Protein family/group databases

CAZyi GH53. Glycoside Hydrolase Family 53.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU25711 ; AAU25711 ; BL00263 .
AAU43089 ; AAU43089 ; BLi04276 .
GeneIDi 3029947.
3101427.
KEGGi bld:BLi04276.
bli:BL00263.
PATRICi 18954382. VBIBacLic203714_4366.

Phylogenomic databases

eggNOGi COG3867.
HOGENOMi HOG000118034.
KOi K01224.
OMAi IRLRLWV.
OrthoDBi EOG6H4K5M.

Enzyme and pathway databases

BioCyci BLIC279010:GJ2P-4230-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q65CX5.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR011683. Glyco_hydro_53.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF07745. Glyco_hydro_53. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
    Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.
  3. "The structure of endo-beta-1,4-galactanase from Bacillus licheniformis in complex with two oligosaccharide products."
    Ryttersgaard C., Le Nours J., Lo Leggio L., Joergensen C.T., Christensen L.L., Bjoernvad M., Larsen S.
    J. Mol. Biol. 341:107-117(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 28-424 IN COMPLEX WITH CALCIUM IONS AND SUBSTRATE ANALOGS, COFACTOR.

Entry informationi

Entry nameiGANA_BACLD
AccessioniPrimary (citable) accession number: Q65CX5
Secondary accession number(s): Q62NF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 25, 2004
Last modified: September 3, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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