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Q65CX4

- BGAL2_BACLD

UniProt

Q65CX4 - BGAL2_BACLD

Protein

Beta-galactosidase GanA

Gene

ganA

Organism
Bacillus licheniformis (strain DSM 13 / ATCC 14580)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 2 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Hydrolyzes oligosaccharides released by the endo-1,4-beta-galactosidase GalA from arabinogalactan type I, a pectic plant polysaccharide. It is unable to use lactose as a sole carbon source By similarity. Catalyzes the hydrolysis of lactose to its constituent monosaccharides glucose and galactose. Possesses a low level of transgalactosylation activity for the production of galacto-oligosaccharides (GOS) from lactose.By similarity1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

    Enzyme regulationi

    Inhibited by hydrolysis end products D-galactose and D-glucose. The hydrolysis of o-nitrophenyl-beta-D-galactopyranoside (ONPG) is slightly activated by monovalent ions, Na+ and K+. Concentrations of these ions in the range of 1-100 mM exert the stimulating effects. The presence of 1 mM Mn2+ together with the presence of 10 mM Na+ slightly stimulates the activity, while presence of 10 mM Mn2+ inhibits the activity by about 40%.1 Publication

    Kineticsi

    1. KM=13.7 mM for ONPG (at 30 degrees Celsius and pH 6.5)1 Publication
    2. KM=169 mM for lactose (at 30 degrees Celsius and pH 6.5)1 Publication

    Vmax=299 µmol/min/mg enzyme with ONPG as substrate (at 30 degrees Celsius and pH 6.5)1 Publication

    Vmax=13 µmol/min/mg enzyme with lactose as substrate (at 30 degrees Celsius and pH 6.5)1 Publication

    pH dependencei

    Optimum pH is 6.5 for both lactose and ONPG hydrolysis. Stable at pH 5-8 and most stable at 6.5, retaining more than 90% and 80% of its activity when incubated at pH 6.5 and 37 degrees Celsius for 5 days and 1 month, respectively.1 Publication

    Temperature dependencei

    Optimum temperature of the activity is 50 degrees Celsius when using both lactose and ONPG as substrates under 10 minutes assay conditions. Stable over a wide range of temperatures (4-42 degrees Celsius), and when kept at these temperatures up to 1 month. Most stable at 37 degrees Celsius, retaining 90% of its activity after 1 month at this temperature. Half-life time of activity of approximately 7 days, 5 hours, and 30 minutes at 55, 60 and 65 degrees Celsius, respectively. Approximately 45% of lactose is hydrolyzed within the first 3 hours of the reaction at 60 degrees Celsius, while about 20% is cleaved at 37 degrees Celsius when employing initial lactose concentration of 50 g/l at pH 6.5. For initial lactose concentrations of 200 and 50 g/l and at temperature of 60 degrees Celsius, the maximum GOS yields are approximately 12% and 7%, respectively. At the initial lactose concentration of 200 g/l, the GOS yields obtained at 60 degrees Celsius are significantly higher than at 37 degrees Celsius, with approximately 12% and 5% of total sugars, respectively.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei105 – 1051SubstrateBy similarity
    Metal bindingi109 – 1091ZincBy similarity
    Binding sitei143 – 1431SubstrateBy similarity
    Active sitei144 – 1441Proton donorBy similarity
    Metal bindingi149 – 1491ZincBy similarity
    Metal bindingi151 – 1511ZincBy similarity
    Metal bindingi154 – 1541ZincBy similarity
    Active sitei308 – 3081NucleophileBy similarity
    Binding sitei316 – 3161SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. galactose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciBLIC279010:GJ2P-4231-MONOMER.

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase GanA (EC:3.2.1.23)
    Short name:
    Beta-gal
    Alternative name(s):
    Beta-1,4-galactooligomerase
    Galactooligomerase
    Gene namesi
    Name:ganA
    Synonyms:galO, lacA
    Ordered Locus Names:BLi04277, BL00264
    OrganismiBacillus licheniformis (strain DSM 13 / ATCC 14580)
    Taxonomic identifieri279010 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000000606: Chromosome, UP000000608: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    Pathology & Biotechi

    Biotechnological usei

    Has potential for partial lactose removal in food products and improving the quality of dairy products by increasing their solubility and sweetness.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 673673Beta-galactosidase GanAPRO_0000367027Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi279010.BL00264.

    Structurei

    3D structure databases

    ProteinModelPortaliQ65CX4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni356 – 3594Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Curated

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000117811.
    KOiK12308.
    OMAiRIQPTED.
    OrthoDBiEOG6GTZGG.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q65CX4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLHGGDYNPD QWLDRPDILA DDIKLMKLAH TNTFSVGIFS WSALEPEEGV    50
    YTFEWLDDIF ESIHRNGGRI ILATPSGARP AWLSQKYPEV LRVNAERVKQ 100
    LHGGRHNHCF TSYVYREKTK EINRMLAERY GSQHALLMWH VSNEYGGECH 150
    CDQCQHAFRD WLKKKYNHDI KSLNDAWWTP FWSHTFNDWS QIESPSPIGE 200
    NAVHGLNLDW RRFVTDQTIS FFQNEIVPLK EITPNIPITT NFMADTHDLI 250
    PFQGLDYSKF AKHLDVISWD AYPAWHNDWE STADLAMKVG FINDLYRSLK 300
    QQPFLLMEST PSAVNWHDFN KAKRPGMHLL SSVQMIAHGS DSILYFQWRK 350
    SRGSSEKFHG AVVGHDNCSE NRVFKEVAKV GQTLEALSEV TGTIRPADVA 400
    ILYDWENHWA LQDAQGFGMK TKRYPQTLHE HYRAFWERDI PVDVITKEQD 450
    FSSYRLLIVP MLYLASEETI ARLKAFAANG GTLVMTYISG IVNESDLTYL 500
    GGWPKDLQEM FGMEPVETDT LYPGDKNAVR YQNRSYELKD YATVLKLSTA 550
    DPEGFYEDDF YADTTAVTSH PYKQGKTYYI GARLSSQFHR DFYGTLIKEL 600
    AIQPALDVKH QPGVSVQVRQ DEENDYIFIM NFTEKRQPVV LASAVKDMLT 650
    GETLAGEVTL EKYEARIAVK AKE 673
    Length:673
    Mass (Da):77,497
    Last modified:March 24, 2009 - v2
    Checksum:iD21163244C361A34
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000002 Genomic DNA. Translation: AAU25712.1.
    AE017333 Genomic DNA. Translation: AAU43090.1.
    RefSeqiYP_006715544.1. NC_006322.1.
    YP_081350.1. NC_006270.3.

    Genome annotation databases

    EnsemblBacteriaiAAU25712; AAU25712; BL00264.
    AAU43090; AAU43090; BLi04277.
    GeneIDi3029925.
    3098321.
    KEGGibld:BLi04277.
    bli:BL00264.
    PATRICi18954384. VBIBacLic203714_4367.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000002 Genomic DNA. Translation: AAU25712.1 .
    AE017333 Genomic DNA. Translation: AAU43090.1 .
    RefSeqi YP_006715544.1. NC_006322.1.
    YP_081350.1. NC_006270.3.

    3D structure databases

    ProteinModelPortali Q65CX4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 279010.BL00264.

    Protein family/group databases

    CAZyi GH42. Glycoside Hydrolase Family 42.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU25712 ; AAU25712 ; BL00264 .
    AAU43090 ; AAU43090 ; BLi04277 .
    GeneIDi 3029925.
    3098321.
    KEGGi bld:BLi04277.
    bli:BL00264.
    PATRICi 18954384. VBIBacLic203714_4367.

    Phylogenomic databases

    eggNOGi COG1874.
    HOGENOMi HOG000117811.
    KOi K12308.
    OMAi RIQPTED.
    OrthoDBi EOG6GTZGG.

    Enzyme and pathway databases

    BioCyci BLIC279010:GJ2P-4231-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProi IPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001084. B-galactosidase. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
      Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
      J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 13 / ATCC 14580.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 13 / ATCC 14580.
    3. "Cloning, purification, and characterization of beta-galactosidase from Bacillus licheniformis DSM 13."
      Juajun O., Nguyen T.H., Maischberger T., Iqbal S., Haltrich D., Yamabhai M.
      Appl. Microbiol. Biotechnol. 89:645-654(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBUNIT, BIOTECHNOLOGY.

    Entry informationi

    Entry nameiBGAL2_BACLD
    AccessioniPrimary (citable) accession number: Q65CX4
    Secondary accession number(s): Q62NE9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 71 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3