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Q65CX4

- BGAL2_BACLD

UniProt

Q65CX4 - BGAL2_BACLD

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Protein

Beta-galactosidase GanA

Gene

ganA

Organism
Bacillus licheniformis (strain DSM 13 / ATCC 14580)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes oligosaccharides released by the endo-1,4-beta-galactosidase GalA from arabinogalactan type I, a pectic plant polysaccharide. It is unable to use lactose as a sole carbon source (By similarity). Catalyzes the hydrolysis of lactose to its constituent monosaccharides glucose and galactose. Possesses a low level of transgalactosylation activity for the production of galacto-oligosaccharides (GOS) from lactose.By similarity1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Enzyme regulationi

Inhibited by hydrolysis end products D-galactose and D-glucose. The hydrolysis of o-nitrophenyl-beta-D-galactopyranoside (ONPG) is slightly activated by monovalent ions, Na+ and K+. Concentrations of these ions in the range of 1-100 mM exert the stimulating effects. The presence of 1 mM Mn2+ together with the presence of 10 mM Na+ slightly stimulates the activity, while presence of 10 mM Mn2+ inhibits the activity by about 40%.1 Publication

Kineticsi

  1. KM=13.7 mM for ONPG (at 30 degrees Celsius and pH 6.5)1 Publication
  2. KM=169 mM for lactose (at 30 degrees Celsius and pH 6.5)1 Publication

Vmax=299 µmol/min/mg enzyme with ONPG as substrate (at 30 degrees Celsius and pH 6.5)1 Publication

Vmax=13 µmol/min/mg enzyme with lactose as substrate (at 30 degrees Celsius and pH 6.5)1 Publication

pH dependencei

Optimum pH is 6.5 for both lactose and ONPG hydrolysis. Stable at pH 5-8 and most stable at 6.5, retaining more than 90% and 80% of its activity when incubated at pH 6.5 and 37 degrees Celsius for 5 days and 1 month, respectively.1 Publication

Temperature dependencei

Optimum temperature of the activity is 50 degrees Celsius when using both lactose and ONPG as substrates under 10 minutes assay conditions. Stable over a wide range of temperatures (4-42 degrees Celsius), and when kept at these temperatures up to 1 month. Most stable at 37 degrees Celsius, retaining 90% of its activity after 1 month at this temperature. Half-life time of activity of approximately 7 days, 5 hours, and 30 minutes at 55, 60 and 65 degrees Celsius, respectively. Approximately 45% of lactose is hydrolyzed within the first 3 hours of the reaction at 60 degrees Celsius, while about 20% is cleaved at 37 degrees Celsius when employing initial lactose concentration of 50 g/l at pH 6.5. For initial lactose concentrations of 200 and 50 g/l and at temperature of 60 degrees Celsius, the maximum GOS yields are approximately 12% and 7%, respectively. At the initial lactose concentration of 200 g/l, the GOS yields obtained at 60 degrees Celsius are significantly higher than at 37 degrees Celsius, with approximately 12% and 5% of total sugars, respectively.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051SubstrateBy similarity
Metal bindingi109 – 1091ZincBy similarity
Binding sitei143 – 1431SubstrateBy similarity
Active sitei144 – 1441Proton donorBy similarity
Metal bindingi149 – 1491ZincBy similarity
Metal bindingi151 – 1511ZincBy similarity
Metal bindingi154 – 1541ZincBy similarity
Active sitei308 – 3081NucleophileBy similarity
Binding sitei316 – 3161SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-4231-MONOMER.

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase GanA (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Beta-1,4-galactooligomerase
Galactooligomerase
Gene namesi
Name:ganA
Synonyms:galO, lacA
Ordered Locus Names:BLi04277, BL00264
OrganismiBacillus licheniformis (strain DSM 13 / ATCC 14580)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000000606: Chromosome, UP000000608: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

Pathology & Biotechi

Biotechnological usei

Has potential for partial lactose removal in food products and improving the quality of dairy products by increasing their solubility and sweetness.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 673673Beta-galactosidase GanAPRO_0000367027Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi279010.BL00264.

Structurei

3D structure databases

ProteinModelPortaliQ65CX4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni356 – 3594Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Curated

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000117811.
KOiK12308.
OMAiRIQPTED.
OrthoDBiEOG6GTZGG.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q65CX4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLHGGDYNPD QWLDRPDILA DDIKLMKLAH TNTFSVGIFS WSALEPEEGV
60 70 80 90 100
YTFEWLDDIF ESIHRNGGRI ILATPSGARP AWLSQKYPEV LRVNAERVKQ
110 120 130 140 150
LHGGRHNHCF TSYVYREKTK EINRMLAERY GSQHALLMWH VSNEYGGECH
160 170 180 190 200
CDQCQHAFRD WLKKKYNHDI KSLNDAWWTP FWSHTFNDWS QIESPSPIGE
210 220 230 240 250
NAVHGLNLDW RRFVTDQTIS FFQNEIVPLK EITPNIPITT NFMADTHDLI
260 270 280 290 300
PFQGLDYSKF AKHLDVISWD AYPAWHNDWE STADLAMKVG FINDLYRSLK
310 320 330 340 350
QQPFLLMEST PSAVNWHDFN KAKRPGMHLL SSVQMIAHGS DSILYFQWRK
360 370 380 390 400
SRGSSEKFHG AVVGHDNCSE NRVFKEVAKV GQTLEALSEV TGTIRPADVA
410 420 430 440 450
ILYDWENHWA LQDAQGFGMK TKRYPQTLHE HYRAFWERDI PVDVITKEQD
460 470 480 490 500
FSSYRLLIVP MLYLASEETI ARLKAFAANG GTLVMTYISG IVNESDLTYL
510 520 530 540 550
GGWPKDLQEM FGMEPVETDT LYPGDKNAVR YQNRSYELKD YATVLKLSTA
560 570 580 590 600
DPEGFYEDDF YADTTAVTSH PYKQGKTYYI GARLSSQFHR DFYGTLIKEL
610 620 630 640 650
AIQPALDVKH QPGVSVQVRQ DEENDYIFIM NFTEKRQPVV LASAVKDMLT
660 670
GETLAGEVTL EKYEARIAVK AKE
Length:673
Mass (Da):77,497
Last modified:March 24, 2009 - v2
Checksum:iD21163244C361A34
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000002 Genomic DNA. Translation: AAU25712.1.
AE017333 Genomic DNA. Translation: AAU43090.1.
RefSeqiYP_006715544.1. NC_006322.1.
YP_081350.1. NC_006270.3.

Genome annotation databases

EnsemblBacteriaiAAU25712; AAU25712; BL00264.
AAU43090; AAU43090; BLi04277.
GeneIDi3029925.
3098321.
KEGGibld:BLi04277.
bli:BL00264.
PATRICi18954384. VBIBacLic203714_4367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000002 Genomic DNA. Translation: AAU25712.1 .
AE017333 Genomic DNA. Translation: AAU43090.1 .
RefSeqi YP_006715544.1. NC_006322.1.
YP_081350.1. NC_006270.3.

3D structure databases

ProteinModelPortali Q65CX4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 279010.BL00264.

Protein family/group databases

CAZyi GH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU25712 ; AAU25712 ; BL00264 .
AAU43090 ; AAU43090 ; BLi04277 .
GeneIDi 3029925.
3098321.
KEGGi bld:BLi04277.
bli:BL00264.
PATRICi 18954384. VBIBacLic203714_4367.

Phylogenomic databases

eggNOGi COG1874.
HOGENOMi HOG000117811.
KOi K12308.
OMAi RIQPTED.
OrthoDBi EOG6GTZGG.

Enzyme and pathway databases

BioCyci BLIC279010:GJ2P-4231-MONOMER.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProi IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view ]
PIRSFi PIRSF001084. B-galactosidase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
    Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.
  3. "Cloning, purification, and characterization of beta-galactosidase from Bacillus licheniformis DSM 13."
    Juajun O., Nguyen T.H., Maischberger T., Iqbal S., Haltrich D., Yamabhai M.
    Appl. Microbiol. Biotechnol. 89:645-654(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBUNIT, BIOTECHNOLOGY.

Entry informationi

Entry nameiBGAL2_BACLD
AccessioniPrimary (citable) accession number: Q65CX4
Secondary accession number(s): Q62NE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: October 1, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3