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Protein

Fiber protein

Gene

L5

Organism
Canine adenovirus serotype 2 (strain Toronto A 26-61) (CAdV-2) (Canine adenovirus 2 (strain Toronto A 26-61))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Forms spikes that protrude from each vertex of the icosahedral capsid. Interacts with host receptor to provide virion initial attachment to target cell. Fiber proteins are shed during virus entry, when virus is still at the cell surface (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral attachment to host adhesion receptor, Viral attachment to host cell, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Fiber protein
Short name:
SPIKE
Alternative name(s):
Protein IV
Gene namesi
ORF Names:L5
OrganismiCanine adenovirus serotype 2 (strain Toronto A 26-61) (CAdV-2) (Canine adenovirus 2 (strain Toronto A 26-61))
Taxonomic identifieri69152 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirusCanine mastadenovirus A
Virus hostiCanis lupus familiaris (Dog) (Canis familiaris) [TaxID: 9615]

Subcellular locationi

  • Virion By similarity
  • Host nucleus By similarity

  • Note: Anchored to the pentons, protrudes from the virion surface.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002218071 – 542Fiber proteinAdd BLAST542

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homotrimer. Interacts (via N-terminal tail region) with pentons (By similarity).By similarity

Structurei

Secondary structure

1542
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi363 – 366Combined sources4
Beta strandi375 – 377Combined sources3
Beta strandi380 – 391Combined sources12
Beta strandi393 – 404Combined sources12
Helixi406 – 408Combined sources3
Beta strandi409 – 411Combined sources3
Beta strandi418 – 424Combined sources7
Beta strandi434 – 436Combined sources3
Beta strandi443 – 445Combined sources3
Helixi458 – 463Combined sources6
Turni467 – 470Combined sources4
Beta strandi474 – 483Combined sources10
Turni484 – 489Combined sources6
Helixi491 – 493Combined sources3
Beta strandi494 – 502Combined sources9
Beta strandi509 – 517Combined sources9
Helixi519 – 521Combined sources3
Beta strandi522 – 524Combined sources3
Beta strandi533 – 539Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J1KX-ray2.30C/D/E/F/H/I/L/M/N/Q/R/S358-542[»]
2J2JX-ray1.50A/B/C/D/E/F358-542[»]
2W9LX-ray2.91C/D/E/F/H/I/L/M/N/Q/R/S358-542[»]
2WBVX-ray1.90A/B/C/D/E/F358-542[»]
ProteinModelPortaliQ65914.
SMRiQ65914.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ65914.

Family & Domainsi

Domaini

The tail region anchors the fiber to penton base capsomers, whereas the shaft, built from several repeated motifs, allows the knob to protrude from the virion.By similarity

Sequence similaritiesi

Belongs to the adenoviridae fiber family.Curated

Family and domain databases

Gene3Di2.10.25.20. 3 hits.
2.60.90.10. 1 hit.
InterProiIPR000931. Adeno_fibre.
IPR000978. Adeno_fibre_knob.
IPR000939. Adenobir_fibre_prot_rpt/shaft.
IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
[Graphical view]
PfamiPF00608. Adeno_shaft. 7 hits.
[Graphical view]
PRINTSiPR00307. ADENOVSFIBRE.
SUPFAMiSSF49835. SSF49835. 1 hit.
SSF51225. SSF51225. 3 hits.

Sequencei

Sequence statusi: Complete.

Q65914-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRTRRALPA NYDPVYPYDA PGSSTQPPFF NNKQGLTESP PGTLAVNVSP
60 70 80 90 100
PLTFSTLGAI KLSTGPGLTL NEGKLQASLG PGLITNTEGQ ITVENVNKVL
110 120 130 140 150
SFTSPLHKNE NTVSLALGDG LEDENGTLKV TFPTPPPPLQ FSPPLTKTGG
160 170 180 190 200
TVSLPLQDSM QVTNGKLGVK PTTYAPPLKK TDQQVSLQVG SGLTVINEQL
210 220 230 240 250
QAVQPPATTY NEPLSKTDNS VSLQVGAGLA VQSGALVATP PPPLTFTSPL
260 270 280 290 300
EKNENTVSLQ VGAGLSVQNN ALVATPPPPL TFAYPLVKND NHVALSAGSG
310 320 330 340 350
LRISGGSLTV ATGPGLSHQN GTIGAVVGAG LKFENNAILA KLGNGLTIRD
360 370 380 390 400
GAIEATQPPA APITLWTGPG PSINGFINDT PVIRCFICLT RDSNLVTVNA
410 420 430 440 450
SFVGEGGYRI VSPTQSQFSL IMEFDQFGQL MSTGNINSTT TWGEKPWGNN
460 470 480 490 500
TVQPRPSHTW KLCMPNREVY STPAATISRC GLDSIAVDGA PSRSIDCMLI
510 520 530 540
INKPKGVATY TLTFRFLNFN RLSGGTLFKT DVLTFTYVGE NQ
Length:542
Mass (Da):57,004
Last modified:November 1, 1997 - v1
Checksum:iB71131829ADA7400
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37498 Genomic DNA. Translation: CAA85723.1.
U77082 Genomic DNA. Translation: AAB38734.1.
PIRiS49219.
RefSeqiAP_000632.1. AC_000020.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37498 Genomic DNA. Translation: CAA85723.1.
U77082 Genomic DNA. Translation: AAB38734.1.
PIRiS49219.
RefSeqiAP_000632.1. AC_000020.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J1KX-ray2.30C/D/E/F/H/I/L/M/N/Q/R/S358-542[»]
2J2JX-ray1.50A/B/C/D/E/F358-542[»]
2W9LX-ray2.91C/D/E/F/H/I/L/M/N/Q/R/S358-542[»]
2WBVX-ray1.90A/B/C/D/E/F358-542[»]
ProteinModelPortaliQ65914.
SMRiQ65914.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ65914.

Family and domain databases

Gene3Di2.10.25.20. 3 hits.
2.60.90.10. 1 hit.
InterProiIPR000931. Adeno_fibre.
IPR000978. Adeno_fibre_knob.
IPR000939. Adenobir_fibre_prot_rpt/shaft.
IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
[Graphical view]
PfamiPF00608. Adeno_shaft. 7 hits.
[Graphical view]
PRINTSiPR00307. ADENOVSFIBRE.
SUPFAMiSSF49835. SSF49835. 1 hit.
SSF51225. SSF51225. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSPIKE_ADECT
AccessioniPrimary (citable) accession number: Q65914
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell (By similarity).By similarity

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.