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Protein

Genome polyprotein

Gene
N/A
Organism
Brome streak virus (strain 11-Cal) (BStV) (Brome streak mosaic rymovirus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Coat protein: involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.By similarity
Nuclear inclusion protein B: an RNA-dependent RNA polymerase that plays an essential role in the virus replication.By similarity
Helper component proteinase: required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity (By similarity).By similarity
Cytoplasmic inclusion protein: has helicase activity. It may be involved in replication (By similarity).By similarity
Both 6K peptides are indispensable for virus replication.By similarity
Nuclear inclusion protein A: has RNA-binding and proteolytic activities.By similarity

Catalytic activityi

Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei698For helper component proteinase activityPROSITE-ProRule annotation1
Active sitei769For helper component proteinase activityPROSITE-ProRule annotation1
Active sitei2082For nuclear inclusion protein A activityPROSITE-ProRule annotation1
Active sitei2121For nuclear inclusion protein A activityPROSITE-ProRule annotation1
Active sitei2193For nuclear inclusion protein A activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1228 – 1235ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Suppressor of RNA silencing, Thiol protease, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 10 chains:
Alternative name(s):
N-terminal protein
Helper component proteinase (EC:3.4.22.45)
Short name:
HC-pro
6 kDa protein 1
Short name:
6K1
6 kDa protein 2
Short name:
6K2
Alternative name(s):
VPg
Nuclear inclusion protein A (EC:3.4.22.44)
Short name:
NI-a
Short name:
NIa
Alternative name(s):
49 kDa proteinase
Short name:
49 kDa-Pro
NIa-pro
Nuclear inclusion protein B (EC:2.7.7.48)
Short name:
NI-b
Short name:
NIb
Alternative name(s):
RNA-directed RNA polymerase
Coat protein
Short name:
CP
OrganismiBrome streak virus (strain 11-Cal) (BStV) (Brome streak mosaic rymovirus)
Taxonomic identifieri117138 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stagePotyviridaeTritimovirus
Virus hostiBromus [TaxID: 4501]
Hordeum murinum (Mouse barley) (Critesion murinum) [TaxID: 97361]
Proteomesi
  • UP000000521 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004199921 – 3093Genome polyproteinAdd BLAST3093
ChainiPRO_00000402331 – 403P1 proteinaseAdd BLAST403
ChainiPRO_0000040234404 – 809Helper component proteinaseAdd BLAST406
ChainiPRO_0000040235810 – 1087Protein P3Add BLAST278
ChainiPRO_00000402361088 – 11386 kDa protein 1Add BLAST51
ChainiPRO_00000402371139 – 1783Cytoplasmic inclusion proteinAdd BLAST645
ChainiPRO_00000402381784 – 18346 kDa protein 2Add BLAST51
ChainiPRO_00000402391835 – 2040Viral genome-linked proteinAdd BLAST206
ChainiPRO_00000402402041 – 2275Nuclear inclusion protein AAdd BLAST235
ChainiPRO_00000402412276 – 2773Nuclear inclusion protein BAdd BLAST498
ChainiPRO_00000402422774 – 3093Coat proteinAdd BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1915O-(5'-phospho-RNA)-tyrosineBy similarity1

Post-translational modificationi

VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase (By similarity).By similarity
Genome polyprotein of potyviruses undergoes post-translational proteolytic processing by the main proteinase NIa-pro resulting in the production of at least ten individual proteins. The P1 proteinase and the HC-pro cleave only their respective C-termini autocatalytically. 6K1 is essential for proper proteolytic separation of P3 from CI (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei403 – 404Cleavage; by P1 proteinaseSequence analysis2
Sitei809 – 810Cleavage; by autolysisPROSITE-ProRule annotation2
Sitei1087 – 1088Cleavage; by NIa-proBy similarity2
Sitei1138 – 1139Cleavage; by NIa-proBy similarity2
Sitei1783 – 1784Cleavage; by NIa-proBy similarity2
Sitei1834 – 1835Cleavage; by NIa-proBy similarity2
Sitei2040 – 2041Cleavage; by NIa-proBy similarity2
Sitei2275 – 2276Cleavage; by NIa-proBy similarity2
Sitei2773 – 2774Cleavage; by NIa-proBy similarity2

Keywords - PTMi

Covalent protein-RNA linkage, Phosphoprotein

Interactioni

Protein-protein interaction databases

MINTiMINT-121113.

Structurei

3D structure databases

ProteinModelPortaliQ65730.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini690 – 809Peptidase C6PROSITE-ProRule annotationAdd BLAST120
Domaini1215 – 1366Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST152
Domaini1367 – 1546Helicase C-terminalPROSITE-ProRule annotationAdd BLAST180
Domaini2041 – 2257Peptidase C4PROSITE-ProRule annotationAdd BLAST217
Domaini2516 – 2639RdRp catalyticPROSITE-ProRule annotationAdd BLAST124

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1316 – 1319DEVH box4
Motifi1883 – 1895Nuclear localization signalSequence analysisAdd BLAST13

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 peptidase C4 domain.PROSITE-ProRule annotation
Contains 1 peptidase C6 domain.PROSITE-ProRule annotationCurated
Contains 1 peptidase S30 domain.Curated
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR001456. HC-pro.
IPR031159. HC_PRO_CPD_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR025910. P1_Ser_Pept_dom.
IPR009003. Peptidase_S1_PA.
IPR001592. Poty_coat.
IPR001730. Potyv_NIa-pro_dom.
IPR013648. PP_Potyviridae.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00863. Peptidase_C4. 1 hit.
PF00851. Peptidase_C6. 1 hit.
PF13611. Peptidase_S76. 1 hit.
PF00767. Poty_coat. 1 hit.
PF08440. Poty_PP. 1 hit.
PF00680. RdRP_1. 1 hit.
[Graphical view]
PRINTSiPR00966. NIAPOTYPTASE.
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51744. HC_PRO_CPD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51436. POTYVIRUS_NIA_PRO. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q65730-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQTHVKQVW RPKTASSKVK TSNMVYLAEE SSPLAKTELK EDAVVAADMS
60 70 80 90 100
VINADDAVYG HALEGKYSER KALPARSNET IVSFASGEDG LFLDRAACGS
110 120 130 140 150
IFKTRTGKDT PIATAIRAAT RRGLAYDIAA QLYMCPKCCS ASDKVLYFDT
160 170 180 190 200
NHNDSCQWYL DNCRAVINKD WTLDVIETYN VFPVLVTEEE ERRTLEAVDI
210 220 230 240 250
ALKPLGTVPV HDVVQVYDQK IGEHKEVERN QEAAHGSEPA IITTTPVLRR
260 270 280 290 300
QCIEKRLLRH VHQANKVIAN TVEIYDLMTE TSQICAEKAI PLVFVDYEKK
310 320 330 340 350
KRIRPRVPLR HVLESNNVDP SDDLYADVVP FLKHYGTCGR PVGVINPRDI
360 370 380 390 400
KPGWSGVVLL QDELPESLHQ ECVDGVFVVQ GIGPDGQLKN ALKTTTGERI
410 420 430 440 450
EYYSSKRRMA VNAHVPKAHS FCKYSSLTET LMELDYLRVI SALADVHDPQ
460 470 480 490 500
CVECHKERNE RSFIENLKYT QQGAQNLFNA GTGMGLAFVA SIASGQLMIQ
510 520 530 540 550
RQQPQSSSSI VDQTPRTNED GSLSHNINLL LTPEVVDIWR SMKQMVHLPN
560 570 580 590 600
RKLYMASFND QFGNFDFIPN STLQGVFPDF TMPVTIALND DDKVETNYRH
610 620 630 640 650
VDKNSTIETC VEGLYTQFDA AYWTKKAAQV HKIQPIDQCG MEIGNIALKL
660 670 680 690 700
CHWEGDVALF SPIIRPTPGH LMFGSTDRLL RIPDMTNARH YVPKVGYCYL
710 720 730 740 750
YLFALAMNFC DGENRVTVDA YINKTCRELG AWPKFGEVLR ALDRMATYYG
760 770 780 790 800
CYDAVVPVML VDHVQRTIHV PSPFGIVQSG MHMIQINNLG DLIKLDTMGA
810 820 830 840 850
SELKEYEIGG FRETYKSITK CVKSKSAFME KLNQDNEWLV DMICNPSTLF
860 870 880 890 900
VLSQLMDTHG LILKDVENSF DRLAALLALK ELGSALGPLL TTRKRVALYM
910 920 930 940 950
QSLSKVDELV PHLGMPTGAM NLLKSEIELI QNAIVEEQDM AEIDRVEGKK
960 970 980 990 1000
SILERRDEMF APCVYNEFIN SFGYVSLPGI AYRLTYTGVG ARIGRGCEHL
1010 1020 1030 1040 1050
KTVWSGSWIP EIHLPENLRS NTWSAIKKYT VYSGGTAWRY MKLKIVESAT
1060 1070 1080 1090 1100
QILVAAVITA IGSWLLKKLL KFIRHEKGRL NEVVVFQSKQ EELFISKFMA
1110 1120 1130 1140 1150
VCFVISTFFS LDMSNAIYSS LTKFRAIFSI LSVGSIYQSG ALEKLEEQLG
1160 1170 1180 1190 1200
HVDTFHEFKL YDHDATHANI APSVQSFGTW LDTRVLAGQQ GCDPLEGRHT
1210 1220 1230 1240 1250
KFEMNKNTRD AIAARVLADK DNEFLVMGHV GCGKSTYFPV ALSKQARVLI
1260 1270 1280 1290 1300
CEPTRVLVTN LHDSMMHTCQ VAPSVMMRNH RCITGSSIMV QTYGFALHYL
1310 1320 1330 1340 1350
VNNPENLQQY DFVLFDEVHH TCAEKVVLYN WLKGRDWGGK IVKLTATDRS
1360 1370 1380 1390 1400
PSAEIKAQKS LDIMTLPTMT PLDFVKEQGL STKADASKHG KVILVFLTSF
1410 1420 1430 1440 1450
REVDSCYDEL KRKENFDAIK ADSRNLRNKT SLADLISECK KEFIYIFATN
1460 1470 1480 1490 1500
ILQTGINIEA DVVVDFGYKI VPTYDVDNRM LTTTRKPVNK ADRIQRLGRV
1510 1520 1530 1540 1550
ARMKAGVAMK IGATIDPEAY DDEVTATEAA LLSFAMQVPP VLRNVNLQIF
1560 1570 1580 1590 1600
QRITREQVVT AARFEHQLSY MVWMVNKDGS MPTKLYDLFS PLLLSQGNMR
1610 1620 1630 1640 1650
LSPYYSSLYD SDTFMTVKNY VDIGYLKHDR TTNQRLPFHC HDVSTTFAMK
1660 1670 1680 1690 1700
VADRFEDSRA PSTYSIRVPA VNLRHTAVKL STDPAQVGMI LVVIGEALVH
1710 1720 1730 1740 1750
QKNILEQLKS TRTQLDNYNS CILVPNWNVR GKLDDAINRV ERNVSILENQ
1760 1770 1780 1790 1800
KNSVEKMSVA RGYDELKELL EENHAVAAHV MYQKGPQKFI DDVLLQKRDF
1810 1820 1830 1840 1850
SWMPYISVGA ACLMAGCAWY MLYRQRAKHE AKFEGKASRV KASKQKAFDD
1860 1870 1880 1890 1900
KMARADNYTY YETTDELHNH AREWNDYPTD WVDKVRKKAN VHAMQFGREA
1910 1920 1930 1940 1950
PRRDVRNDRP FFNFYGIDEK LYDTVTFHDM AASFSVEQPI TAMEVEEAFE
1960 1970 1980 1990 2000
KIYLNRQEDE AFFDHPMPKK ILAEFKGKDG KVINVEMEPH NPRKANRRGL
2010 2020 2030 2040 2050
PVGYADHRGE FRQAKPAEEG PIKFERKALN PKATPYAVFE SKALYGGPRC
2060 2070 2080 2090 2100
YEHITNNQVL LAGPSGYLNG LITGSKLLAP YHFVKDISSD SQDPSRMIAR
2110 2120 2130 2140 2150
FGTYNLGNIL NLQVVKFTMI DLIGLDLPVE FQPRRTLKCF RVPVIGEKAV
2160 2170 2180 2190 2200
LVLSRYSKEG WKSCVSAETE ITPYGENEEL LWRHRITTEV GDCGATMVAL
2210 2220 2230 2240 2250
SDQKIVGFHS LGGISMNYFV PVTQELLDFL SSKTEKPLVP WRFSEDQVDV
2260 2270 2280 2290 2300
GGLYIHNDFD KFPFVKTIQK LVGFQNGHMI KYCGEGFTPV ARSENRLSRQ
2310 2320 2330 2340 2350
HVISGQRESF IHFVEASSKW RPLITPMLGR LQPSALNREA YYKDVLKYDK
2360 2370 2380 2390 2400
PIRLGTVHEE AFQSAVINVI RILENAGFER GGVKACFDYG KIFNDLNLDA
2410 2420 2430 2440 2450
AMGALYAGKK KDYFVEATDE EIEEMFLRSA GKICANGHGV WSALLKAELR
2460 2470 2480 2490 2500
PAEKVAANKT RTFTSAPIDI LFGAKAVVDD FNKQFYKRHL LGPWTVGINK
2510 2520 2530 2540 2550
FNKGWDLLAR SLMRYEWFID ADGSQFDSSI TPLLMNAVLT IRLYFMERDD
2560 2570 2580 2590 2600
ITELMLRNLY TQIISTCMLA EDGLIVQKHR GNNSGQPSTV VDNTLCLMIA
2610 2620 2630 2640 2650
MEYARQRAIS DGHLNMQMRY VCNGDDLLIN ANEEAKDVVQ GKYEQYIKEL
2660 2670 2680 2690 2700
ELNYCFDDAF QSIEGVEFMS HKFMLRNGIY IPKLARHRIV AILEWQRSAE
2710 2720 2730 2740 2750
PQAIKSAILA ACVEAFGYDD LTELIREYAI SLEPVWGSFL PTDGEIEQLY
2760 2770 2780 2790 2800
FEGIAKQEVA RCLAGVDDVC KFESAASGTN EAVDEVLKAA GDDEALARAN
2810 2820 2830 2840 2850
AAATSGATTP AQNVGAGTTT PAKATPQSGR RPSFGSLIDN PIGGNGVQDV
2860 2870 2880 2890 2900
ADRTSGIVFP VPTRKSTSLY LPPKVKLRAT PERIEKVRKY LPDPQQIDLR
2910 2920 2930 2940 2950
YSTQQELNDW IKASADGLGQ TEEAFIDNIL PGWIVHCIVN TTSSENRKAG
2960 2970 2980 2990 3000
SWRCVTNAGT ADEEQVLYDI EPMYSAANPT MRAIMRHFSD LARLVIAESF
3010 3020 3030 3040 3050
KQGRPLIPKG YIKAGVLDAS SAAAACDFVV RDRHDTATFV QVQNQVLVNR
3060 3070 3080 3090
VSGITNRLFA QAMPSAGANE DMARHDAQDA AEGIHNLGGA RAF
Length:3,093
Mass (Da):348,106
Last modified:November 1, 1996 - v1
Checksum:i50F775CEF7ABCCB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48506 Genomic RNA. Translation: CAA88417.1.
RefSeqiNP_612585.1. NC_003501.1.

Genome annotation databases

GeneIDi935897.
KEGGivg:935897.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48506 Genomic RNA. Translation: CAA88417.1.
RefSeqiNP_612585.1. NC_003501.1.

3D structure databases

ProteinModelPortaliQ65730.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-121113.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi935897.
KEGGivg:935897.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR001456. HC-pro.
IPR031159. HC_PRO_CPD_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR025910. P1_Ser_Pept_dom.
IPR009003. Peptidase_S1_PA.
IPR001592. Poty_coat.
IPR001730. Potyv_NIa-pro_dom.
IPR013648. PP_Potyviridae.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00863. Peptidase_C4. 1 hit.
PF00851. Peptidase_C6. 1 hit.
PF13611. Peptidase_S76. 1 hit.
PF00767. Poty_coat. 1 hit.
PF08440. Poty_PP. 1 hit.
PF00680. RdRP_1. 1 hit.
[Graphical view]
PRINTSiPR00966. NIAPOTYPTASE.
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51744. HC_PRO_CPD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51436. POTYVIRUS_NIA_PRO. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPOLG_BSTV1
AccessioniPrimary (citable) accession number: Q65730
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.