Q65729 (POLG_BSTVG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Genome polyprotein Cleaved into the following 3 chains: Nuclear inclusion protein A Short name=NI-A Short name=NIA EC=3.4.22.44 Alternative name(s): 49 kDa proteinase Short name=49 kDa-Pro Nuclear inclusion protein B Short name=NI-B Short name=NIB Alternative name(s): RNA-directed RNA polymerase EC=2.7.7.48 Capsid protein Short name=CP Alternative name(s): Coat protein
Organism	Brome streak virus (strain German) (BStV) (Brome streak mosaic rymovirus)
Taxonomic identifier	117139 [NCBI]
Taxonomic lineage	Viruses › ssRNA positive-strand viruses, no DNA stage › Potyviridae › Tritimovirus
Virus host	Bromus [TaxID: 4501] Hordeum murinum (Mouse barley) (Critesion murinum) [TaxID: 97361]

Protein attributes

Sequence length	942 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Nuclear inclusion protein B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication By similarity. Nuclear inclusion protein A has RNA-binding and proteolytic activities By similarity.
Catalytic activity	Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved. Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Capsid protein: Virion Potential.
Post-translational modification	The viral RNA of potyviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing by the main proteinase NIa-pro resulting in the production of at least ten individual proteins By similarity.
Sequence similarities	Belongs to the potyviruses polyprotein family. Contains 1 peptidase C4 domain. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Virion
Ligand	Nucleotide-binding
Molecular function	Capsid protein Hydrolase Nucleotidyltransferase Protease RNA-directed RNA polymerase Thiol protease Transferase
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 108	›108	Nuclear inclusion protein A By similarity		PRO_0000040243
	Chain	109 – 606	498	Nuclear inclusion protein B By similarity		PRO_0000040244
	Chain	607 – 942	336	Capsid protein By similarity		PRO_0000040245
Regions
	Domain	1 – 90	90	Peptidase C4
	Domain	349 – 472	124	RdRp catalytic
Sites
	Active site	26	1	For nuclear inclusion protein A activity By similarity
	Site	108 – 109	2	Cleavage; by NIa-pro By similarity
	Site	606 – 607	2	Cleavage; by NIa-pro By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q65729 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1AF1509108F2504D	FASTA	942	105,547
	10 20 30 40 50 60 ETEITPYGEN EELLWRHRIT TEVGDCGATM VALSDQKIVG FHSLGGISMN YFVPVTQELL 70 80 90 100 110 120 DFLNSKTEKP LVPWRFSEDQ VDVGGLYIHN DFDKFPFVKT IQKLVGFQNG HMIKYCGEGF 130 140 150 160 170 180 TPVARSENRL SRQHVISGQR ESFIHFVEAS AKWRPIVTPM LGRLQPSALN REAYYKDVLK 190 200 210 220 230 240 YDKPIRLGTV HEEAFQSAVI NVIRILENAG FERGGVKACF DYGKIFNDLN LDAAMGALYA 250 260 270 280 290 300 GKKKDYFVEA TDEEIEEMFL RSAGKICANG HGVWSALLKA ELRPAEKVAA NKTRTFTSAP 310 320 330 340 350 360 IDILFGAKAV VDDFNKQFYK RHLLGPWTVG INKFNKGWDL LARSLMRYEW FIDADGSQFD 370 380 390 400 410 420 SSITPLLMNA VLTIRLYFME RDDITELMLR NLYTQIISTC MLAEDGLIVQ KHRGNNSGQP 430 440 450 460 470 480 STVVDNTLCL MIAMEYARQR AISDGHLNMQ MRYVCNGDDL LINANEEAKE VVQNKYEQYI 490 500 510 520 530 540 KELELNYCFD DAFQSIEGVE FMSHKFMLRN GIYIPKLARH RIVAILEWQR SAEPQAIKSA 550 560 570 580 590 600 ILAACVEAFG YDDSTELIRE YAISLEPVWG SFLPTDGEIE QLYLEGIAKQ EVARCLAGVD 610 620 630 640 650 660 DVCKFESAAP GTNEAVDEML KAAGDDEALA RANATSTSDT IPPARNVGAD TTTPAKANPP 670 680 690 700 710 720 SGRRPSSRSL IDNSIGGNGV QDVADRTSGI VFPVPTRKST SLYLTPKVKL RATTEELRKY 730 740 750 760 770 780 ESTSLNPQQI DLRYSTQQEL NDWIKASADG LGQTEEAFID NILPGWIVHC IVNTTSSENR 790 800 810 820 830 840 KAGSWRCVTN AGTADEEQVL YDIEPMYSAA NPTMRAIMRH FSDLAGLVIA ESFKQGRPLI 850 860 870 880 890 900 PKGYIKAGVL DASSAARACD FVVRDRHDTA TFVQVQNQVL VNRVSGITNW LFAQQCLALV 910 920 930 940 LTRTWRAMTL RCRRGHPQPR LSASFLEVST SCRARRRLGV QR

References

[1]	"Sequence of the 3'-terminal region of the RNA of a mite transmitted potyvirus from Hordeum murinum L." Schubert J., Rabenstein F. Eur. J. Plant Pathol. 101:123-132(1995) [Agricola: IND20484543] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Potyvirus proteins: a wealth of functions." Urcuqui-Inchima S., Haenni A.L., Bernardi F. Virus Res. 74:157-175(2001) [PubMed: 11226583] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78485 mRNA. Translation: CAA55237.1.
3D structure databases
ProteinModelPortal	Q65729.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR001730. Peptidase_C4. IPR001592. Poty_coat. IPR001205. RNA-dir_pol_picornavirus. IPR007094. RNA-dir_pol_PSvirus. [Graphical view]
Pfam	PF00863. Peptidase_C4. 1 hit. PF00767. Poty_coat. 1 hit. PF00680. RdRP_1. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS51436. POTYVIRUS_NIA_PRO. 1 hit. PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POLG_BSTVG

Accession

Primary (citable) accession number: Q65729

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program