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Q65709 (NSP1_ROTBB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Non-structural protein 1

Short name=NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name=NS53
OrganismRotavirus A (isolate Cow/United States/B223/1983 G10-P8[11]-I2-Rx-Cx-Mx-A13-Nx-Tx-E2-Hx) (RV-A)
Taxonomic identifier10930 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostBos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation By similarity.

Subunit structure

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity.

Subcellular location

Host cytoplasmhost cytoskeleton By similarity.

Domain

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.

Sequence similarities

Belongs to the rotavirus A NSP1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491Non-structural protein 1
PRO_0000369069

Regions

Zinc finger42 – 7938 Potential
Region1 – 8181RNA-binding By similarity
Region82 – 17695Important for cytoskeleton localization By similarity
Region316 – 491176Interaction with IRF3 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65709 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4E6FBCF4466484FF

FASTA49158,748
        10         20         30         40         50         60 
MATFKDACYH YKKLNKLNGL VLKLGANDAW RPAPVAKYKG WCLDCCQHTD LTYCRGCALF 

        70         80         90        100        110        120 
HVCQWCSRYN RCFLDEEPHL LRMRTFRNEI NKEDVEGLIN MYNIIFPINE RVVDKFINNV 

       130        140        150        160        170        180 
KQRKCRNELL IEWYNHLLLP ITLQALSIEL EGDVYYIFGY YDCMGKENQT PFHFVNMINR 

       190        200        210        220        230        240 
YDRLLLDDKN FDRMMHLPVA LQQEYALRYF SKSRFISQIK REMNRHDFSD NLMEERDNPM 

       250        260        270        280        290        300 
SFMQVTRNCV SAHMNDNDWN ERCKLIGDAR NYMELMKSAY TEHYSISNRC KLFTIYKLNI 

       310        320        330        340        350        360 
ISKLVKPNYI FSNHGLCALD VNNCKWCKID NHYEIWNDFR LRKIYNNMMN FIRALVKSNT 

       370        380        390        400        410        420 
NVGHCSSHEL VYKCISSVFI VWKIEKWNDS VRTLFEYLEP VEINHVEYVL LDHELSWEMS 

       430        440        450        460        470        480 
GVIMQIMNGK VPRILSFDDV KKIMGAIIYD WFDVRYMRET PVIVSTTNEL RKLNKDNNLM 

       490 
DGYDYELSDI E 

« Hide

References

[1]"Molecular biology of rotaviruses. IX. Conservation and divergence in genome segment 5."
Xu L.I., Tian Y., Tarlow O., Harbour D.A., McCrae M.A.
J. Gen. Virol. 75:3413-3421(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z12105 Genomic RNA. Translation: CAA78090.1.
PIRS31784.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamPF00981. Rota_NS53. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNSP1_ROTBB
AccessionPrimary (citable) accession number: Q65709
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families