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Q65701

- NSP3_ROTBN

UniProt

Q65701 - NSP3_ROTBN

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Protein
Non-structural protein 3
Gene
N/A
Organism
Rotavirus A (strain Cow/United States/NCDV-Lincoln/1969 G6-P6[1]-I2-R2-C2-M2-Ax-N2-T6-E2-Hx) (RV-A) (Rotavirus A (strain Nebraska calf diarrhea virus))
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3
Short name:
NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name:
NS34
OrganismiRotavirus A (strain Cow/United States/NCDV-Lincoln/1969 G6-P6[1]-I2-R2-C2-M2-Ax-N2-T6-E2-Hx) (RV-A) (Rotavirus A (strain Nebraska calf diarrhea virus))
Taxonomic identifieri36439 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiBos taurus (Bovine) [TaxID: 9913]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Non-structural protein 3
PRO_0000369446Add
BLAST

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ65701.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 149146RNA-binding By similarity
Add
BLAST
Regioni150 – 20657Dimerization By similarity
Add
BLAST
Regioni170 – 23465Interaction with ZC3H7B By similarity
Add
BLAST
Regioni208 – 313106Interaction with EIF4G1 By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili166 – 23772 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP3 family.

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.

Sequencei

Sequence statusi: Complete.

Q65701-1 [UniParc]FASTAAdd to Basket

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MSKMESTQQM ASSIINTSFE AAVVAATSTL ELMGIQYDYN EVYTRVKSKF    50
DYVMDDSGVK NNLLGKAATY DQALNGKFGS AARNRNWMAD TRTTARLDED 100
VNKLRMMLSS KGIDQKMRVL NACFNVKRVP GKSSSIIKCT RLMRDKIERG 150
EVEVDDSFVE EKMEVDTIDW KSRYEQLEKR FESLKQRVNE KYTSWVQKAK 200
KVNENMYSLQ NVISQQQSQI ADLQNYCNKL EVDLQNKISS LVSSVEWYLK 250
SMELPDEIKT DIEQQLNSID VINPINAIDD FESLIRNIIL DYDRIFLMFK 300
GLMRQCNYEY TYE 313
Length:313
Mass (Da):36,371
Last modified:November 1, 1996 - v1
Checksum:iCD708FBBE5BC257E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81429 mRNA. Translation: CAA57188.1.
PIRiS51709.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X81429 mRNA. Translation: CAA57188.1 .
PIRi S51709.

3D structure databases

ProteinModelPortali Q65701.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002873. Rotavirus_NSP3.
[Graphical view ]
Pfami PF01665. Rota_NSP3. 1 hit.
[Graphical view ]
SUPFAMi SSF69903. SSF69903. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative nucleotide and amino acid sequence analysis of the sequence-specific RNA-binding rotavirus nonstructural protein NSP3."
    Rao C.D., Das M., Ilango P., Lalwani R., Rao B.S., Gowda K.
    Virology 207:327-333(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiNSP3_ROTBN
AccessioniPrimary (citable) accession number: Q65701
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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