Q65657 (POL2_BAMMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Genome polyprotein 2

Cleaved into the following 2 chains:

1. Helper component proteinase
   Short name=HC-pro
   EC=3.4.22.45
2. 70 kDa protein

Gene names

Name:

RNA2

Organism

Barley mild mosaic virus (strain UK-F) (BaMMV)

Taxonomic identifier

103901 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Potyviridae › Bymovirus

Virus host

Hordeum vulgare (Barley) [TaxID: 4513]

Protein attributes

Sequence length	894 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.
Post-translational modification	The viral RNA2 of bymoviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing resulting in the production of at least two individual proteins. The HC-pro cleaves its C-terminus autocatalytically Potential.
Sequence similarities	Belongs to the bymoviruses polyprotein 2 family. Contains 1 peptidase C6 domain.

Ontologies

Keywords
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	viral capsid Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Helper component proteinase Potential		PRO_0000040562
	Chain	230 – 894	665	70 kDa protein		PRO_0000040563
Sites
	Active site	117	1	For helper component proteinase activity By similarity
	Active site	189	1	For helper component proteinase activity By similarity
	Site	229 – 230	2	Cleavage; by HC-PRO protease Potential

Sequences

	Sequence		Length	Mass (Da)
	Q65657 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 05A3B3B371E76243	FASTA	894	98,349
	10 20 30 40 50 60 MMMNSTIRQG WQQVLRRFSI PASGDRLIVS NSTDQPIGLF GAFDTSLQTL SQVTNDPEIL 70 80 90 100 110 120 KQKSNIPTHL DIASVLETSP RSFPWVFLTN SFCTFGGSIH AQNLQAFATA EFKSGFCYMN 130 140 150 160 170 180 LLIPLSFDII DAHADSFRGF VEQLPDTLGA YPSLSMVLNV MLHAATRFPE IVASPVPTIA 190 200 210 220 230 240 FDAESLQFHV TDKRGVPGMW NILKACRVYE LLSLAADGIG CEYMLYPVGA APQYSFWKKS 250 260 270 280 290 300 MDHFISDRFV EFLAMQGLLA SALEQDYKTH DARDALLTAL QNAGYTNVVA RERRFPNGHD 310 320 330 340 350 360 PSTVWLNLNE APISEKLTEL KRYLLVGHRS DEIADITHNV HQHVFEVLKT MSVQFSKTTN 370 380 390 400 410 420 AYNRARFEVN HEVIWNAEYG RSSQQNAELE ALVLFLNRQS LEIENILRRT TSPVVVTNWQ 430 440 450 460 470 480 PDVPPAAPEI SEGEPTHAVA TPITEAPTHA TPVEVVNLPP TRSYWAETLV GVLTAILGTI 490 500 510 520 530 540 FALLTRALIR PKRLRRKSTF PWVSLDSGDE DDDHSGGGGG SPQTPGGQPP ASPAPGTHQS 550 560 570 580 590 600 RFSVQDIASD TSLLSVDLDE DTLSQYDETF QTIRRALFEN SFGDILQNSA RWISTLEAMA 610 620 630 640 650 660 LADGNAPYTL LAQYLNGIEE AYTNFRNTGH ISRATLSGFF VLEDSLRAAG IAFGGTTPTQ 670 680 690 700 710 720 TIQNQSADSP ARRWKTRFEQ IACELGDASI KSLADLADII DTERERGDLT QFDVLAASSI 730 740 750 760 770 780 SSLCRAVRII SDTTDPNTQL ALVENATAMQ NNINAILGTN VSIPFLSATR RLLITRRIQE 790 800 810 820 830 840 AGAESRSGAT PDTIQQLADA ELAEIVSEAN MFNEMAASQR DIANATREAT IREHVLSPVN 850 860 870 880 890 ALANVGMAAA FFRSGGLRSR AFHPTMPTMP GSPAAIGRPM FQAFRGRGHR LNRR

References

[1]

"The complete nucleotide sequence of RNA-2 of a fungally-transmitted UK isolate of barley mild mosaic bymovirus and identification of amino acid combinations possibly involved in fungus transmission." Peerenboom E., Jacobi V., Antoniw J.F., Schlichter U., Cartwright E.J., Steinbiss H.H., Adams M.J. Virus Res. 40:149-159(1996) [PubMed: 8725111] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X90904 Genomic RNA. Translation: CAA62412.1.
RefSeq	NP_604490.1. NC_003482.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	963864.
Family and domain databases
InterPro	IPR001456. Peptidase_C6. IPR001337. TMV-like_coat. [Graphical view]
Pfam	PF00851. Peptidase_C6. 1 hit. PF00721. TMV_coat. 1 hit. [Graphical view]
SUPFAM	SSF47195. TMV_coat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

POL2_BAMMU

Accession

Primary (citable) accession number: Q65657

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program