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Protein

Probable allantoate deiminase

Gene

AAH

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the catabolism of purine nucleotides. The sequential activity of AAH, UGLYAH and UAH allows a complete purine berakdown without the intermediate generation of urea.1 Publication

Catalytic activityi

Allantoate + H2O = (S)-ureidoglycine + NH3 + CO2.1 Publication

Cofactori

Mn2+By similarity

Kineticsi

  1. KM=83 µM for allantoate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi122Manganese 1By similarity1
    Metal bindingi131Manganese 1By similarity1
    Metal bindingi131Manganese 2By similarity1
    Metal bindingi168Manganese 2By similarity1
    Metal bindingi234Manganese 1By similarity1
    Metal bindingi454Manganese 2By similarity1

    GO - Molecular functioni

    • allantoate deiminase activity Source: UniProtKB
    • metal ion binding Source: UniProtKB-KW
    • metallopeptidase activity Source: InterPro

    GO - Biological processi

    • allantoin catabolic process Source: UniProtKB
    • purine nucleobase catabolic process Source: EnsemblPlants
    • ureide catabolic process Source: EnsemblPlants

    Keywordsi

    Molecular functionHydrolase
    Biological processPurine metabolism
    LigandManganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable allantoate deiminase (EC:3.5.3.9)
    Alternative name(s):
    Allantoate amidohydrolase
    Short name:
    OsAAH
    Gene namesi
    Name:AAH
    Ordered Locus Names:Os06g0665500, LOC_Os06g45480
    ORF Names:OsJ_22281, P0473H04.24
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
    Proteomesi
    • UP000059680 Componenti: Chromosome 6

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 32Sequence analysisAdd BLAST32
    ChainiPRO_000042344333 – 491Probable allantoate deiminaseAdd BLAST459

    Proteomic databases

    PaxDbiQ655X8.
    PRIDEiQ655X8.

    Expressioni

    Gene expression databases

    GenevisibleiQ655X8. OS.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi39947.LOC_Os06g45480.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ655X8.
    SMRiQ655X8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi385 – 388Poly-Ala4
    Compositional biasi483 – 489Poly-Ala7

    Sequence similaritiesi

    Belongs to the peptidase M20A family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410II0M. Eukaryota.
    COG0624. LUCA.
    HOGENOMiHOG000241291.
    InParanoidiQ655X8.
    KOiK02083.
    OMAiCDEEGTR.
    OrthoDBiEOG093608AX.

    Family and domain databases

    CDDicd03884. M20_bAS. 1 hit.
    InterProiView protein in InterPro
    IPR010158. Amidase_Cbmase.
    IPR001261. ArgE/DapE_CS.
    IPR036264. Bact_exopeptidase_dim_dom.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    PfamiView protein in Pfam
    PF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    SUPFAMiSSF55031. SSF55031. 1 hit.
    TIGRFAMsiTIGR01879. hydantase. 1 hit.
    PROSITEiView protein in PROSITE
    PS00758. ARGE_DAPE_CPG2_1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q655X8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MALLLSYPRR HPSIHLLILS AYALFLLPIL DGLELGGDGL YREILRDETV
    60 70 80 90 100
    LRLKELGKIS DGEGYLERTF LSPASIRASA VIISWMKDAG LTTWIDQMGN
    110 120 130 140 150
    IHGRFEPTNS TKEALLIGSH MDTVIDAGMY DGALGIISAI SALKVLKVTG
    160 170 180 190 200
    RLQRLTRPVE VIAFSDEEGV RFQTTFLGSA AVAGTLPESI LQVSDKSGTT
    210 220 230 240 250
    VQDVLKLNSL EGTANALGEV RYSPESVGSY VEVHIEQGPV LEALRYPLGV
    260 270 280 290 300
    VKGIAGQTRL KVIINGSQGH AGTVPMKLRR DPMVAAAELV LTLETLCKEP
    310 320 330 340 350
    NKFLTYDEEC GCFTEESLAG LVCTVGELLT WPSASNVIPG QVNFTVDIRA
    360 370 380 390 400
    MDDKVRETIV TSFSRLVLQR CDDRLVDCAV EQKHAAAATP CDAELTSRLE
    410 420 430 440 450
    RATRSTISSM AAGVRRAGGE TPVLMSGAGH DAMAMARLTK VGMLFVRCRG
    460 470 480 490
    GVSHSPEESV MDDDVWAAGL ALVNFIDQNA VDAAAATAAE S
    Length:491
    Mass (Da):52,716
    Last modified:October 25, 2004 - v1
    Checksum:i7F631B0C1FBEE731
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti414 – 415VR → FP in EEE66180 (PubMed:15685292).Curated2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AP003628 Genomic DNA. Translation: BAD45389.1.
    AP008212 Genomic DNA. Translation: BAF20218.1.
    AP014962 Genomic DNA. Translation: BAS99030.1.
    CM000143 Genomic DNA. Translation: EEE66180.1.
    AK073262 mRNA. Translation: BAG93368.1.
    RefSeqiXP_015644100.1. XM_015788614.1.
    UniGeneiOs.11403.

    Genome annotation databases

    EnsemblPlantsiOS06T0665500-01; OS06T0665500-01; OS06G0665500.
    GeneIDi4341777.
    GrameneiOS06T0665500-01; OS06T0665500-01; OS06G0665500.
    KEGGiosa:4341777.

    Similar proteinsi

    Entry informationi

    Entry nameiAAH_ORYSJ
    AccessioniPrimary (citable) accession number: Q655X8
    Secondary accession number(s): A0A0P0WZU4, B9FQD8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 18, 2013
    Last sequence update: October 25, 2004
    Last modified: October 25, 2017
    This is version 97 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families