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Q65526

- VP3_ROTBS

UniProt

Q65526 - VP3_ROTBS

Protein

Protein VP3

Gene
N/A
Organism
Rotavirus C (isolate Cow/Japan/Shintoku/1991) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.By similarity

    Catalytic activityi

    GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
    S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
    3. mRNA guanylyltransferase activity Source: UniProtKB-EC
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. viral process Source: InterPro

    Keywords - Molecular functioni

    Methyltransferase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA capping, mRNA processing

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein VP3
    Including the following 2 domains:
    mRNA guanylyltransferase (EC:2.7.7.50)
    mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
    OrganismiRotavirus C (isolate Cow/Japan/Shintoku/1991) (RV-C)
    Taxonomic identifieri33723 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]

    Subcellular locationi

    Virion Curated
    Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Potential.Curated

    GO - Cellular componenti

    1. viral nucleocapsid Source: InterPro

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 695695Protein VP3PRO_0000369871Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with VP1 Potential. Interacts with VP2.Curated

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the rotavirus VP3 family.Curated

    Family and domain databases

    InterProiIPR011181. VP3_Rotav.
    [Graphical view]
    PfamiPF06929. Rotavirus_VP3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
    PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q65526-1 [UniParc]FASTAAdd to Basket

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    MRVLGLFERG NNLNFADTYV YTWNKQYSFH ENAFLISNQV ATTIIIYLDK    50
    EIVNQVNEAF NLLNSNGIPA LIIKSDHIGI FTSSNFTYDW QNKIIYFHEY 100
    TYYKNNEFIV SDEFWLNTSI QDLLPYKVLF FERGLRKLYE GEEYILYNTA 150
    TDDDIIYKYI YEKDVIMSGN DYSKLYDTKS FKNFVHFMRL LRMRFAVPFD 200
    QLSNRVTRSR AFAKSKIHIG LRNESIPQAL DNIHHYWINY SANGMRVSEL 250
    KGSGSYSEKK ISEFDIGQFK NYMNFLTLMF YIKNMKKKPS CTIIGAAPGY 300
    WIPSMKKYFN IVTYDDKHVD STEHYNRYFT DDDIASVKTN GVYIDVRSDF 350
    KNYDWKKRRQ LVEEETMRWL SITYKLLENR YVEAVLLKMT AMDIEIPDGY 400
    FVHFPTTYRK SEYYLLVDKQ TVKRPKIKIT KSLAYGAINT IFSDNVFISG 450
    KYSLKGKTEG VLALYCLSNT INPKEKVVQY ANSFSGTCMT VRLNNTYILN 500
    KIIDFKTNAD YTFLPSDFQC SIKTVLTSYR GYAGVFGYAI TKDLKSDGNN 550
    HIYIIPNARD DDNFDTFASH LGLSRYSHSK RFSESATTMS GYLFRDMVSG 600
    KENMEDTDTE NLASGHVFNA IAHYRFDYTY DIVGWLKLHK MRKFRVKSNI 650
    YGEHTDDEIR NAIEAAYVYY LLDGDEVGKE YAKRIMEIWD VQTWG 695
    Length:695
    Mass (Da):81,517
    Last modified:November 1, 1996 - v1
    Checksum:i1EF9F1ECFFAD3328
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U26552 Genomic DNA. Translation: AAB01673.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U26552 Genomic DNA. Translation: AAB01673.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011181. VP3_Rotav.
    [Graphical view ]
    Pfami PF06929. Rotavirus_VP3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
    PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of VP3 and VP4 genes of a bovine group C rotavirus: molecular evidence for a new P type."
      Jiang B., Gentsch J.R., Tsunemitsu H., Saif L.J., Glass R.I.
      Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiVP3_ROTBS
    AccessioniPrimary (citable) accession number: Q65526
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3