Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q65525 (VP4_ROTBS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer capsid protein VP4
Alternative name(s):
Hemagglutinin

Cleaved into the following 2 chains:

  1. Outer capsid protein VP8*
  2. Outer capsid protein VP5*
OrganismRotavirus C (isolate Cow/Japan/Shintoku/1991) (RV-C)
Taxonomic identifier33723 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostBos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length733 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.

Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.

VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies By similarity.

Subunit structure

VP4 is a homotrimer Potential.

Subcellular location

Outer capsid protein VP4: Virion. Host rough endoplasmic reticulum Potential. Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.

Outer capsid protein VP8*: Virion By similarity. Note: Outer capsid protein By similarity.

Outer capsid protein VP5*: Virion By similarity. Note: Outer capsid protein By similarity.

Post-translational modification

Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.

Sequence similarities

Belongs to the rotavirus VP4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 733733Outer capsid protein VP4
PRO_0000369873
Chain1 – 228228Outer capsid protein VP8* By similarity
PRO_0000369874
Chain229 – 733505Outer capsid protein VP5* By similarity
PRO_0000369875

Regions

Region390 – 41021Hydrophobic; possible role in virus entry into host cell Potential
Coiled coil485 – 51935 Potential
Compositional bias714 – 7174Poly-Asn

Sites

Site228 – 2292Cleavage Potential
Site244 – 2452Cleavage Potential

Amino acid modifications

Glycosylation281N-linked (GlcNAc...); by host Potential
Glycosylation391N-linked (GlcNAc...); by host Potential
Glycosylation1201N-linked (GlcNAc...); by host Potential
Glycosylation1621N-linked (GlcNAc...); by host Potential
Glycosylation1891N-linked (GlcNAc...); by host Potential
Glycosylation3011N-linked (GlcNAc...); by host Potential
Glycosylation3791N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
Q65525 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 67C1B19AFCD1631C

FASTA73382,495
        10         20         30         40         50         60 
MASSLYRQLI SQNYYSTGNE ILLDQQTNKT TVDYVDAGNY TYAQLPPTTW GAESTYESAF 

        70         80         90        100        110        120 
SAPEITGPYT NTVIKLSDLS DSNVWVLYQK PTSTVKLLKN GPESYSWNLA AFELWYGKAN 

       130        140        150        160        170        180 
TTVTSDYYSG MTNSEKSVEV DHDSLVLFWN EGSTALSNKV INFSWNVGGV LIKLTSNTRI 

       190        200        210        220        230        240 
DICMANMDNF TSDSFNWEEW THNFPRSASM NIYTDYYLAS VDPYSQIRAL QQPIITTVEM 

       250        260        270        280        290        300 
KMVKVKREGS INVDEVVSKD SLWQEVRYVR DITLQCKIES EVVKGGGWGY DYTSVAFKTI 

       310        320        330        340        350        360 
NHTYSYTRAG EAVNAHVTIS FNNLKERSYG GSLPTDFKIG RFDIIDVDTY MYIDYWDDSE 

       370        380        390        400        410        420 
IFKNMVYVRD LRADMGGFNY SSAMSYYFRI PVGQYPGLHS SGVRFTYERS LLSQQFTDQV 

       430        440        450        460        470        480 
ALNSMRFVFR ATSSDGWFMT AGNINARRIA SGTGFAYSDG YVTETVGTVS FISLIPSNPN 

       490        500        510        520        530        540 
YQTPIASSST VRMDLERKIN DLRNDFNELA SSVALGDILS LAMSPLTFAN LLESVPAIAS 

       550        560        570        580        590        600 
SVKDVAANVM KKFKTTKMFK KAAKPKYKEY IIGDLLEDVT NLPRSTTAMD FDDITSAVMV 

       610        620        630        640        650        660 
STTNRLQLTD VETLSEIVAR SADDFIPNRA YRMIEDGMVH EATPNGVFSY DLATLQQRNF 

       670        680        690        700        710        720 
DMEKFMQLAS KSPVISAIVD FATLKAMRDT YGVSTDIMYK LVASDAPTIV SFINNNNPLI 

       730 
RNRIEGLLRQ CRI 

« Hide

References

[1]"Sequence analysis of VP3 and VP4 genes of a bovine group C rotavirus: molecular evidence for a new P type."
Jiang B., Gentsch J.R., Tsunemitsu H., Saif L.J., Glass R.I.
Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U26551 Genomic DNA. Translation: AAB01672.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000416. Haemagglutinin_VP4.
[Graphical view]
PfamPF00426. VP4_haemagglut. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVP4_ROTBS
AccessionPrimary (citable) accession number: Q65525
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families