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Q65525

- VP4_ROTBS

UniProt

Q65525 - VP4_ROTBS

Protein

Outer capsid protein VP4

Gene
N/A
Organism
Rotavirus C (isolate Cow/Japan/Shintoku/1991) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.By similarity
    Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.By similarity
    VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei228 – 2292CleavageSequence Analysis
    Sitei244 – 2452CleavageSequence Analysis

    GO - Biological processi

    1. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
    2. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin

    Keywords - Biological processi

    Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid protein VP4
    Alternative name(s):
    Hemagglutinin
    Cleaved into the following 2 chains:
    OrganismiRotavirus C (isolate Cow/Japan/Shintoku/1991) (RV-C)
    Taxonomic identifieri33723 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]

    Subcellular locationi

    Chain Outer capsid protein VP4 : Virion. Host rough endoplasmic reticulum Curated
    Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.By similarity
    Chain Outer capsid protein VP8* : Virion By similarity
    Note: Outer capsid protein.By similarity
    Chain Outer capsid protein VP5* : Virion By similarity
    Note: Outer capsid protein.By similarity

    GO - Cellular componenti

    1. host cell rough endoplasmic reticulum Source: UniProtKB-SubCell
    2. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host endoplasmic reticulum, Outer capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 733733Outer capsid protein VP4PRO_0000369873Add
    BLAST
    Chaini1 – 228228Outer capsid protein VP8*By similarityPRO_0000369874Add
    BLAST
    Chaini229 – 733505Outer capsid protein VP5*By similarityPRO_0000369875Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi28 – 281N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi39 – 391N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi120 – 1201N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi162 – 1621N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi189 – 1891N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi301 – 3011N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi379 – 3791N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.By similarity

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    VP4 is a homotrimer.Curated

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni390 – 41021Hydrophobic; possible role in virus entry into host cellSequence AnalysisAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili485 – 51935Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi714 – 7174Poly-Asn

    Sequence similaritiesi

    Belongs to the rotavirus VP4 family.Curated

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR000416. Haemagglutinin_VP4.
    [Graphical view]
    PfamiPF00426. VP4_haemagglut. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q65525-1 [UniParc]FASTAAdd to Basket

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    MASSLYRQLI SQNYYSTGNE ILLDQQTNKT TVDYVDAGNY TYAQLPPTTW    50
    GAESTYESAF SAPEITGPYT NTVIKLSDLS DSNVWVLYQK PTSTVKLLKN 100
    GPESYSWNLA AFELWYGKAN TTVTSDYYSG MTNSEKSVEV DHDSLVLFWN 150
    EGSTALSNKV INFSWNVGGV LIKLTSNTRI DICMANMDNF TSDSFNWEEW 200
    THNFPRSASM NIYTDYYLAS VDPYSQIRAL QQPIITTVEM KMVKVKREGS 250
    INVDEVVSKD SLWQEVRYVR DITLQCKIES EVVKGGGWGY DYTSVAFKTI 300
    NHTYSYTRAG EAVNAHVTIS FNNLKERSYG GSLPTDFKIG RFDIIDVDTY 350
    MYIDYWDDSE IFKNMVYVRD LRADMGGFNY SSAMSYYFRI PVGQYPGLHS 400
    SGVRFTYERS LLSQQFTDQV ALNSMRFVFR ATSSDGWFMT AGNINARRIA 450
    SGTGFAYSDG YVTETVGTVS FISLIPSNPN YQTPIASSST VRMDLERKIN 500
    DLRNDFNELA SSVALGDILS LAMSPLTFAN LLESVPAIAS SVKDVAANVM 550
    KKFKTTKMFK KAAKPKYKEY IIGDLLEDVT NLPRSTTAMD FDDITSAVMV 600
    STTNRLQLTD VETLSEIVAR SADDFIPNRA YRMIEDGMVH EATPNGVFSY 650
    DLATLQQRNF DMEKFMQLAS KSPVISAIVD FATLKAMRDT YGVSTDIMYK 700
    LVASDAPTIV SFINNNNPLI RNRIEGLLRQ CRI 733
    Length:733
    Mass (Da):82,495
    Last modified:November 1, 1996 - v1
    Checksum:i67C1B19AFCD1631C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U26551 Genomic DNA. Translation: AAB01672.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U26551 Genomic DNA. Translation: AAB01672.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR000416. Haemagglutinin_VP4.
    [Graphical view ]
    Pfami PF00426. VP4_haemagglut. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of VP3 and VP4 genes of a bovine group C rotavirus: molecular evidence for a new P type."
      Jiang B., Gentsch J.R., Tsunemitsu H., Saif L.J., Glass R.I.
      Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiVP4_ROTBS
    AccessioniPrimary (citable) accession number: Q65525
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3