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Q65525

- VP4_ROTBS

UniProt

Q65525 - VP4_ROTBS

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Protein

Outer capsid protein VP4

Gene
N/A
Organism
Rotavirus C (isolate Cow/Japan/Shintoku/1991) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.
Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.
VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei228 – 2292Cleavage Reviewed prediction
Sitei244 – 2452Cleavage Reviewed prediction

GO - Biological processi

  1. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid protein VP4
Alternative name(s):
Hemagglutinin
Cleaved into the following 2 chains:
OrganismiRotavirus C (isolate Cow/Japan/Shintoku/1991) (RV-C)
Taxonomic identifieri33723 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]

Subcellular locationi

Chain Outer capsid protein VP4 : Virion. Host rough endoplasmic reticulum Reviewed prediction
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.
Chain Outer capsid protein VP8* : Virion By similarity
Note: Outer capsid protein By similarity.
Chain Outer capsid protein VP5* : Virion By similarity
Note: Outer capsid protein By similarity.

GO - Cellular componenti

  1. host cell rough endoplasmic reticulum Source: UniProtKB-SubCell
  2. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 733733Outer capsid protein VP4PRO_0000369873Add
BLAST
Chaini1 – 228228Outer capsid protein VP8* By similarityPRO_0000369874Add
BLAST
Chaini229 – 733505Outer capsid protein VP5* By similarityPRO_0000369875Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi28 – 281N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi39 – 391N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi120 – 1201N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi162 – 1621N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi189 – 1891N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi301 – 3011N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi379 – 3791N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

VP4 is a homotrimer Reviewed prediction.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni390 – 41021Hydrophobic; possible role in virus entry into host cell Reviewed predictionAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili485 – 51935 Reviewed predictionAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi714 – 7174Poly-Asn

Sequence similaritiesi

Belongs to the rotavirus VP4 family.

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR000416. Haemagglutinin_VP4.
[Graphical view]
PfamiPF00426. VP4_haemagglut. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q65525-1 [UniParc]FASTAAdd to Basket

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MASSLYRQLI SQNYYSTGNE ILLDQQTNKT TVDYVDAGNY TYAQLPPTTW    50
GAESTYESAF SAPEITGPYT NTVIKLSDLS DSNVWVLYQK PTSTVKLLKN 100
GPESYSWNLA AFELWYGKAN TTVTSDYYSG MTNSEKSVEV DHDSLVLFWN 150
EGSTALSNKV INFSWNVGGV LIKLTSNTRI DICMANMDNF TSDSFNWEEW 200
THNFPRSASM NIYTDYYLAS VDPYSQIRAL QQPIITTVEM KMVKVKREGS 250
INVDEVVSKD SLWQEVRYVR DITLQCKIES EVVKGGGWGY DYTSVAFKTI 300
NHTYSYTRAG EAVNAHVTIS FNNLKERSYG GSLPTDFKIG RFDIIDVDTY 350
MYIDYWDDSE IFKNMVYVRD LRADMGGFNY SSAMSYYFRI PVGQYPGLHS 400
SGVRFTYERS LLSQQFTDQV ALNSMRFVFR ATSSDGWFMT AGNINARRIA 450
SGTGFAYSDG YVTETVGTVS FISLIPSNPN YQTPIASSST VRMDLERKIN 500
DLRNDFNELA SSVALGDILS LAMSPLTFAN LLESVPAIAS SVKDVAANVM 550
KKFKTTKMFK KAAKPKYKEY IIGDLLEDVT NLPRSTTAMD FDDITSAVMV 600
STTNRLQLTD VETLSEIVAR SADDFIPNRA YRMIEDGMVH EATPNGVFSY 650
DLATLQQRNF DMEKFMQLAS KSPVISAIVD FATLKAMRDT YGVSTDIMYK 700
LVASDAPTIV SFINNNNPLI RNRIEGLLRQ CRI 733
Length:733
Mass (Da):82,495
Last modified:November 1, 1996 - v1
Checksum:i67C1B19AFCD1631C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U26551 Genomic DNA. Translation: AAB01672.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U26551 Genomic DNA. Translation: AAB01672.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR000416. Haemagglutinin_VP4.
[Graphical view ]
Pfami PF00426. VP4_haemagglut. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of VP3 and VP4 genes of a bovine group C rotavirus: molecular evidence for a new P type."
    Jiang B., Gentsch J.R., Tsunemitsu H., Saif L.J., Glass R.I.
    Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiVP4_ROTBS
AccessioniPrimary (citable) accession number: Q65525
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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