Q654U4 (GUN16_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 16
EC=3.2.1.4

Alternative name(s):
Endo-1,4-beta glucanase 16

Gene names

Ordered Locus Names:	Os06g0247900, LOC_Os06g13830
ORF Names:	OJ1136_C11.27

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.
Sequence caution	The sequence BAD45673.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAF19200.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q654U4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Derived from EST data. No experimental confirmation available.

Isoform 2 (identifier: Q654U4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-158: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 538	512	Endoglucanase 16		PRO_0000249293
Sites
	Active site	431	1	By similarity
	Active site	483	1	By similarity
	Active site	492	1	By similarity
Natural variations
	Alternative sequence	1 – 158	158	Missing in isoform 2.		VSP_020389

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: 94ED0101E3408692	FASTA	538	58,893
	10 20 30 40 50 60 MRWRRVGDVV AVALLLGAAA AAAAAAARHD YEEALRKSLL YFEAQRSGRL PHGQRVAWRD 70 80 90 100 110 120 HSGLTDGLEQ GVDLVGGYYD AGDHVKFGLP MAFTVTMLSW SLLEYGADVA AAGELAHALD 130 140 150 160 170 180 AIKWGTDYFI KAHTKPHELW AEVGDGDTDH YCWQRPEDMT TSRQAYKVDR RRPGSDVAGE 190 200 210 220 230 240 TAAAMAAASI VFRQSNPHYS HLLLHHAQQL FEFADTYRGK YDSSIAEVKS YYASVSGYHD 250 260 270 280 290 300 ELLWAALWLH RATGRAAYLD YAVDNADEFG GTGWAITEFS WDVKYAGVQI LAARLLMRGE 310 320 330 340 350 360 HEERHRGTLE RYREKAEHYV CACMGRNAAG GADANVERSP GGMLYVRQWN NMQYVTNAAF 370 380 390 400 410 420 LLSAYSDYLA GAGDGDGDGG GGVATCVGGG GAGAGEVFAA AREQVDYVLG SNPRGMSYLV 430 440 450 460 470 480 GYGERFPARV HHRAASIVPY KDSKEFIGCA QGFDDWFGRR GANPNVVVGA IVGGPDRRDR 490 500 510 520 530 FRDDRENYMQ TEACTYNTAP MVGMFAMLNR LSRQESPSTT TTTTATTSSP EMGLSVNR
	Isoform 2 [UniParc]. Checksum: 3DE3C0B8934314A1	FASTA	380	41,530
	10 20 30 40 50 60 MTTSRQAYKV DRRRPGSDVA GETAAAMAAA SIVFRQSNPH YSHLLLHHAQ QLFEFADTYR 70 80 90 100 110 120 GKYDSSIAEV KSYYASVSGY HDELLWAALW LHRATGRAAY LDYAVDNADE FGGTGWAITE 130 140 150 160 170 180 FSWDVKYAGV QILAARLLMR GEHEERHRGT LERYREKAEH YVCACMGRNA AGGADANVER 190 200 210 220 230 240 SPGGMLYVRQ WNNMQYVTNA AFLLSAYSDY LAGAGDGDGD GGGGVATCVG GGGAGAGEVF 250 260 270 280 290 300 AAAREQVDYV LGSNPRGMSY LVGYGERFPA RVHHRAASIV PYKDSKEFIG CAQGFDDWFG 310 320 330 340 350 360 RRGANPNVVV GAIVGGPDRR DRFRDDRENY MQTEACTYNT APMVGMFAML NRLSRQESPS 370 380 TTTTTTATTS SPEMGLSVNR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004027 Genomic DNA. Translation: BAD45673.1. Sequence problems. AP008212 Genomic DNA. Translation: BAF19200.1. Sequence problems. AK065325 mRNA. No translation available.
RefSeq	NP_001057286.1. NM_001063821.1.
UniGene	Os.48095.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	Q654U4.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4340645.
KEGG	osa:4340645.
Organism-specific databases
Gramene	Q654U4.
Phylogenomic databases
eggNOG	euNOG00265.
OMA	KEFIGCA.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN16_ORYSJ

Accession

Primary (citable) accession number: Q654U4
Secondary accession number(s): Q0DD73

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	September 5, 2006
Last modified:	April 3, 2013
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program