Q65474 (NCAP_BEFVB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoprotein
Short name=NP

Alternative name(s):
Nucleocapsid protein
Short name=Protein N

Gene names

Name:

N

Organism

Bovine ephemeral fever virus (strain BB7721) (BEFV) [Reference proteome]

Taxonomic identifier

928297 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Dimarhabdovirus supergroup › Ephemerovirus › Bovine ephemeral fever virus

Virus host

Bos taurus (Bovine) [TaxID: 9913]
Bubalus bubalis (Domestic water buffalo) [TaxID: 89462]
Culicoides [TaxID: 58271]
Syncerus caffer (Cape buffalo) [TaxID: 9970]

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Encapsidates the genome, protecting it from nucleases. If expressed without protein P it binds non-specifically RNA and therefore can bind it's own mRNA. Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation. The soluble N-P complex encapsidates specifically the genomic RNA, with protein N protecting the genome like a pearl necklace. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for viral transcription and replication. Protein N binds protein P in the NC through a different interaction, and can be phosphorylated. Subsequent viral replication is dependent on intracellular concentration of newly synthesized protein N. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases By similarity.
Subunit structure	Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA By similarity.
Subcellular location	Virion. Host cytoplasm By similarity.
Sequence similarities	Belongs to the ephemerovirus nucleocapsid protein family.

Ontologies

Keywords
Cellular component	Host cytoplasm Virion
Ligand	RNA-binding Viral nucleoprotein
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell ribonucleoprotein complex Inferred from electronic annotation. Source: UniProtKB-KW viral nucleocapsid Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Nucleoprotein		PRO_0000297608

Sequences

	Sequence		Length	Mass (Da)
	Q65474 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 906A6D17C11BA2ED	FASTA	431	49,163
	10 20 30 40 50 60 MYCTLNKKEI KAVKPTDAIP PQYPKEFFIN GNGKKPTLRV PQGKLDLPTV RELVFGGLER 70 80 90 100 110 120 GELVLSHVIR YLYLVGERIT EKLEGDWISF GVNIGRRNQE INVWNFYEVI IEDDQTIDGR 130 140 150 160 170 180 RANNVDENDD VWLTLALLAY YRLGRSANQN HRNNLLIKLN AQIKGYRKDA PNIIDDVAVH 190 200 210 220 230 240 GSWVTNSEFC KIAAGFDMFM NRFKNNKYAH VRFGTVASRY KDAAGLMALG HACDVTGLTI 250 260 270 280 290 300 GEILDWIFVS NVGEDVVKIM EEGNEIDDPY SYMPYMMDMG ISNKSPYSSI SCPHIYTFLH 310 320 330 340 350 360 LVGTLLTSER SKHARMVSEH NLQNIKMNAF VVSYVKSNKA ALTKAFLKSE DRDYEKRQED 370 380 390 400 410 420 GSNDDEDEDE SGDDDDFGAM PKSSDPMEWF IFLESNHFIL PEKVTEFCIR ECKKIQNARP 430 NTIGKYLASI V

References

[1]	"Structural and antigenic analysis of the nucleoprotein of bovine ephemeral fever rhabdovirus." Walker P.J., Wang Y., Cowley J.A., McWilliam S.M., Prehaud C.J. J. Gen. Virol. 75:1889-1899(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U04166 Genomic RNA. Translation: AAA19450.1. AF234533 Genomic RNA. Translation: AAG10409.1.
RefSeq	NP_065398.1. NC_002526.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	911726.
Family and domain databases
Gene3D	1.10.3570.10. 1 hit. 1.10.3610.10. 1 hit.
InterPro	IPR000448. Rhabdo_ncapsid. IPR023331. Rhabdovirus_ncapsid_C. IPR023330. Rhabdovirus_ncapsid_N. [Graphical view]
Pfam	PF00945. Rhabdo_ncap. 1 hit. [Graphical view]
ProDom	PD002087. Rhabd_nucleocap. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF140809. SSF140809. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NCAP_BEFVB

Accession

Primary (citable) accession number: Q65474

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program