Probable alpha-glucosidase Os06g0675700 precursor - Oryza sativa subsp. japonica (Rice)

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Reviewed, UniProtKB/Swiss-Prot Q653V7 (AGLU_ORYSJ)

Last modified January 19, 2010. Version 28. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Probable alpha-glucosidase Os06g0675700
    EC=3.2.1.20
Alternative name(s):
    Maltase

Gene names

Ordered Locus Names:	Os06g0675700, LOC_Os06g46284
ORF Names:	B1153E06.2

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	885 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Sequence similarities	Belongs to the glycosyl hydrolase 31 family.
Caution	Was isolated and reported to have peptide:N-glycanase activity (Ref.3). However, its strong sequence similarity with alpha-glucosidase proteins suggest that it is not its function in vivo.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

33

Chain

852

Probable alpha-glucosidase Os06g0675700

PRO_0000249005

Sites

Active site

1

By similarity

Active site

1

By similarity

Active site

1

Proton donor By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

1

G → S AA sequence Ref.3

Sequence conflict

1

S → L AA sequence Ref.3

Sequence conflict

1

A → P Ref.2

Sequence conflict

1

N → S Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q653V7-1 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 34ABDF8A194C75A5
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885

96,336

        10         20         30         40         50         60 
MMGSPPAPPA RRLGALAVFL LALFLAAPWG VDCGYNVASV AGSKNRLRAR LELAGGGGGA 

        70         80         90        100        110        120 
APELGPDVRR LSLTASLETD SRLHVRITDA DHPRWEVPQD VIPRPSPDSF LAATRPGGGR 

       130        140        150        160        170        180 
VLSTATSDLT FAIHTSPFRF TVTRRSTGDV LFDTTPNLVF KDRYLELTSS LPPPGRASLY 

       190        200        210        220        230        240 
GLGEQTKRTF RLQRNDTFTL WNSDIAAGNV DLNLYGSHPF YMDVRSGGGG GGGAAHGVLL 

       250        260        270        280        290        300 
LNSNGMDVIY GGSYVTYKVI GGVLDFYFFA GPSPLAVVDQ YTQLIGRPAP MPYWSFGFHQ 

       310        320        330        340        350        360 
CRYGYKNVAD LEGVVAGYAK ARIPLEVMWT DIDYMDAYKD FTLDPVNFPA DRMRPFVDRL 

       370        380        390        400        410        420 
HRNGQKFVVI IDPGINVNTT YGTFVRGMKQ DIFLKWNGSN YLGVVWPGNV YFPDFLNPRA 

       430        440        450        460        470        480 
AEFWAREIAA FRRTLPVDGL WVDMNEISNF VDPPPLNAID DPPYRINNSG VRRPINNKTV 

       490        500        510        520        530        540 
PASAVHYGGV AEYDAHNLFG FLEARATHDA LLRDTGRRPF VLSRSTFVGS GRYTAHWTGD 

       550        560        570        580        590        600 
NAATWEDLHY SINTMLSFGL FGIPMIGADI CGFGGNTTEE LCSRWIQLGA FYPFSRDHSA 

       610        620        630        640        650        660 
IGTVRRELYL WESVARSARK ALGLRYRLLP YLYTLMYEAH TTGAPIARPL FFSYPGDVET 

       670        680        690        700        710        720 
YGIDRQFLLG RGVLVSPVLE PGATTVTAYF PAGRWFSLYD FSLAVATKTG KRVTLPAPAD 

       730        740        750        760        770        780 
TVNVHVAGGN ILTLQQPALT SSRVRQSVVH LLVALADDGT ATGDLFLDDG ESPEMAGPRS 

       790        800        810        820        830        840 
RWSQIKFSGA TESGGGVVRV RSHVVHDSYA PSRTMAIGKV VLMGLRSAAP PKGFAVYANG 

       850        860        870        880 
VQVNASTAVG GAAGSPEKGA LGVAHVSGLT LVVGQEFDLK VVMTY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Oryza sativa nipponbare chromosome 6 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[3]	"Developmentally regulated expression of a peptide:N-glycanase during germination of rice seeds (Oryza sativa) and its purification and characterization." Chang T., Kuo M.-C., Khoo K.-H., Inoue S., Inoue Y. J. Biol. Chem. 275:129-134(2000) [PubMed: 10617595] [Abstract] Cited for: PROTEIN SEQUENCE OF 34-43.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004989 Genomic DNA. Translation: BAD45910.1. AK119824 mRNA. No translation available.
RefSeq	NP_001058347.1.
UniGene	Os.49616
3D structure databases
SMR	Q653V7. Positions 26-880.
ModBase	Search...
Protein family/group databases
CAZy	GH31. Glycoside Hydrolase Family 31.
Genome annotation databases
GeneID	4341824.
KEGG	osa:4341824.
Organism-specific databases
Gramene	Q653V7.
Phylogenomic databases
PhylomeDB	Q653V7.
Family and domain databases
InterPro	IPR000322. Glyco_hydro_31. IPR017853. Glyco_hydro_catalytic_core. [Graphical view]
PANTHER	PTHR22762. Glyco_hydro_31. 1 hit.
Pfam	PF01055. Glyco_hydro_31. 1 hit. [Graphical view]
PROSITE	PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit. PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGLU_ORYSJ

Accession

Primary (citable) accession number: Q653V7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	October 25, 2004
Last modified:	January 19, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents