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Protein

Probable alpha-glucosidase Os06g0675700

Gene

Os06g0675700

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei443By similarity1
Active sitei446By similarity1
Active sitei540Proton donorBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiR-OSA-189085. Digestion of dietary carbohydrate.
R-OSA-5357572. Lysosomal glycogen catabolism.
R-OSA-6798695. Neutrophil degranulation.

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha-glucosidase Os06g0675700 (EC:3.2.1.20)
Alternative name(s):
Maltase
Gene namesi
Ordered Locus Names:Os06g0675700, LOC_Os06g46284
ORF Names:B1153E06.2, OsJ_22347Imported
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 6

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1163102.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 331 PublicationAdd BLAST33
ChainiPRO_000024900534 – 885Probable alpha-glucosidase Os06g0675700Add BLAST852

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi195N-linked (GlcNAc...)Sequence analysis1
Glycosylationi378N-linked (GlcNAc...)Sequence analysis1
Glycosylationi397N-linked (GlcNAc...)Sequence analysis1
Glycosylationi467N-linked (GlcNAc...)Sequence analysis1
Glycosylationi477N-linked (GlcNAc...)Sequence analysis1
Glycosylationi576N-linked (GlcNAc...)Sequence analysis1
Glycosylationi844N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ653V7.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os06g46284.1.

Chemistry databases

BindingDBiQ653V7.

Structurei

3D structure databases

ProteinModelPortaliQ653V7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1065. Eukaryota.
COG1501. LUCA.
InParanoidiQ653V7.
KOiK01187.
OMAiDNVSGWL.
OrthoDBiEOG093602P1.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
InterProiIPR013785. Aldolase_TIM.
IPR031727. Gal_mutarotase_N.
IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR030458. Glyco_hydro_31_AS.
IPR030459. Glyco_hydro_31_CS.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
PF16863. NtCtMGAM_N. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q653V7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMGSPPAPPA RRLGALAVFL LALFLAAPWG VDCGYNVASV AGSKNRLRAR
60 70 80 90 100
LELAGGGGGA APELGPDVRR LSLTASLETD SRLHVRITDA DHPRWEVPQD
110 120 130 140 150
VIPRPSPDSF LAATRPGGGR VLSTATSDLT FAIHTSPFRF TVTRRSTGDV
160 170 180 190 200
LFDTTPNLVF KDRYLELTSS LPPPGRASLY GLGEQTKRTF RLQRNDTFTL
210 220 230 240 250
WNSDIAAGNV DLNLYGSHPF YMDVRSGGGG GGGAAHGVLL LNSNGMDVIY
260 270 280 290 300
GGSYVTYKVI GGVLDFYFFA GPSPLAVVDQ YTQLIGRPAP MPYWSFGFHQ
310 320 330 340 350
CRYGYKNVAD LEGVVAGYAK ARIPLEVMWT DIDYMDAYKD FTLDPVNFPA
360 370 380 390 400
DRMRPFVDRL HRNGQKFVVI IDPGINVNTT YGTFVRGMKQ DIFLKWNGSN
410 420 430 440 450
YLGVVWPGNV YFPDFLNPRA AEFWAREIAA FRRTLPVDGL WVDMNEISNF
460 470 480 490 500
VDPPPLNAID DPPYRINNSG VRRPINNKTV PASAVHYGGV AEYDAHNLFG
510 520 530 540 550
FLEARATHDA LLRDTGRRPF VLSRSTFVGS GRYTAHWTGD NAATWEDLHY
560 570 580 590 600
SINTMLSFGL FGIPMIGADI CGFGGNTTEE LCSRWIQLGA FYPFSRDHSA
610 620 630 640 650
IGTVRRELYL WESVARSARK ALGLRYRLLP YLYTLMYEAH TTGAPIARPL
660 670 680 690 700
FFSYPGDVET YGIDRQFLLG RGVLVSPVLE PGATTVTAYF PAGRWFSLYD
710 720 730 740 750
FSLAVATKTG KRVTLPAPAD TVNVHVAGGN ILTLQQPALT SSRVRQSVVH
760 770 780 790 800
LLVALADDGT ATGDLFLDDG ESPEMAGPRS RWSQIKFSGA TESGGGVVRV
810 820 830 840 850
RSHVVHDSYA PSRTMAIGKV VLMGLRSAAP PKGFAVYANG VQVNASTAVG
860 870 880
GAAGSPEKGA LGVAHVSGLT LVVGQEFDLK VVMTY
Length:885
Mass (Da):96,336
Last modified:October 25, 2004 - v1
Checksum:i34ABDF8A194C75A5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34G → S AA sequence (PubMed:10617595).Curated1
Sequence conflicti43S → L AA sequence (PubMed:10617595).Curated1
Sequence conflicti177A → P in AK119824 (PubMed:12869764).Curated1
Sequence conflicti445N → S in AK119824 (PubMed:12869764).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP004989 Genomic DNA. Translation: BAD45910.1.
AP008212 Genomic DNA. Translation: BAF20261.1.
AP014962 Genomic DNA. Translation: BAS99103.1.
CM000143 Genomic DNA. Translation: EEE66212.1.
AK119824 mRNA. No translation available.
RefSeqiXP_015643120.1. XM_015787634.1.
UniGeneiOs.98112.

Genome annotation databases

EnsemblPlantsiOS06T0675700-01; OS06T0675700-01; OS06G0675700.
GeneIDi4341824.
GrameneiOS06T0675700-01; OS06T0675700-01; OS06G0675700.
KEGGiosa:4341824.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP004989 Genomic DNA. Translation: BAD45910.1.
AP008212 Genomic DNA. Translation: BAF20261.1.
AP014962 Genomic DNA. Translation: BAS99103.1.
CM000143 Genomic DNA. Translation: EEE66212.1.
AK119824 mRNA. No translation available.
RefSeqiXP_015643120.1. XM_015787634.1.
UniGeneiOs.98112.

3D structure databases

ProteinModelPortaliQ653V7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os06g46284.1.

Chemistry databases

BindingDBiQ653V7.
ChEMBLiCHEMBL1163102.

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Proteomic databases

PaxDbiQ653V7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS06T0675700-01; OS06T0675700-01; OS06G0675700.
GeneIDi4341824.
GrameneiOS06T0675700-01; OS06T0675700-01; OS06G0675700.
KEGGiosa:4341824.

Phylogenomic databases

eggNOGiKOG1065. Eukaryota.
COG1501. LUCA.
InParanoidiQ653V7.
KOiK01187.
OMAiDNVSGWL.
OrthoDBiEOG093602P1.

Enzyme and pathway databases

ReactomeiR-OSA-189085. Digestion of dietary carbohydrate.
R-OSA-5357572. Lysosomal glycogen catabolism.
R-OSA-6798695. Neutrophil degranulation.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
InterProiIPR013785. Aldolase_TIM.
IPR031727. Gal_mutarotase_N.
IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR030458. Glyco_hydro_31_AS.
IPR030459. Glyco_hydro_31_CS.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
PF16863. NtCtMGAM_N. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGLU_ORYSJ
AccessioniPrimary (citable) accession number: Q653V7
Secondary accession number(s): Q0DA62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 25, 2004
Last modified: November 30, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was isolated and reported to have peptide:N-glycanase activity (PubMed:10617595). However, its strong sequence similarity with alpha-glucosidase proteins suggest that it is not its function in vivo.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.