Reviewed, UniProtKB/Swiss-Prot Q65329 (POL2_BAMMA)

Names and origin

Protein names

Recommended name:
    Genome polyprotein 2
Cleaved into the following 2 chains:
    1- Recommended name:
            Helper component proteinase
                Short name=HC-pro
              EC=3.4.22.45
    2- Recommended name:
            70 kDa protein

Gene names

Name:

RNA2

Organism

Barley mild mosaic virus (strain ASL) (BaMMV)

Taxonomic identifier

103899 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Potyviridae › Bymovirus

Virus host

Hordeum vulgare (Barley) [TaxID: 4513]

Protein attributes

Sequence length	893 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.
Post-translational modification	The viral RNA2 of bymoviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing resulting in the production of at least two individual proteins. The HC-pro cleaves its C-terminus autocatalytically Potential.
Sequence similarities	Belongs to the bymoviruses polyprotein 2 family. Contains 1 peptidase C6 domain.

Ontologies

Keywords
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Cellular component	viral capsid Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Helper component proteinase Potential		PRO_0000040558
	Chain	230 – 893	664	70 kDa protein		PRO_0000040559
Sites
	Active site	117	1	For helper component proteinase activity By similarity
	Active site	189	1	For helper component proteinase activity By similarity
	Site	229 – 230	2	Cleavage; by HC-PRO protease Potential

Sequences

	Sequence		Length	Mass (Da)
	Q65329-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D6EB74D9D6CF6407	FASTA	893	98,329
	10 20 30 40 50 60 MMMNSMIRQG WQQVLRRFSI PTSGDRLIVS NSTDQPIGLF GAFDTSLQTL SQVTNDPEVL 70 80 90 100 110 120 KQKSNIPTHL DVASVLEASP RSFPWVFLTN SFCTFGGSIH AQNLQAFATA EFKSGFCYMN 130 140 150 160 170 180 LLIPLSFDII DAHADSFRGF VEQLPDTLGA YPSLSMVLNV MLHAATRFPE IVASPIPTIA 190 200 210 220 230 240 FDAESLQFHV TDKRGVPGMW NILKACRVYE LLSLAADGIG CEYMLYPVGA APQYSFWKKS 250 260 270 280 290 300 MDHFTSDRFV EFLAMQDLLA SALEQDYATH DARDALLSAL QNAGYTNVVA RERRFPNGHD 310 320 330 340 350 360 PSIVWLNLSE APISEKLTEL KRYLLVGHRS DDTADITHNV HQHVFEVLKT MSVQFSKTTN 370 380 390 400 410 420 AYNRARFEVN HKVIWNAEYG RGPQQNAELE ALVLFLNRQS LEIENILHRT TSPVVVTSWK 430 440 450 460 470 480 PDVPPAAPEI KEEEPTHAIA TPITEAPSHV TPVEVVNLPP TRSYWAETLV GILTAILGTV 490 500 510 520 530 540 FAFLTRALIR AKRLRRKSTF PWVTLNSGDD DDDQSGGGGG GPQTPGGQPP VPHTRGTHQS 550 560 570 580 590 600 RFSVQDIASD TSLLSVDLDE DTLSQYDETF QKIRRALFET SFADILQNSA RWISTLEAMA 610 620 630 640 650 660 LADGNAPYTL LAQYLNGIEE AYTNFRNTGH ISRATLSGFF ALEDNLRAAG IAFGTTTPTQ 670 680 690 700 710 720 TIQNQFADSP ARRWKTRFEQ IACELGDASI KSLADLADII DTERERGDLT QFDVLAASSI 730 740 750 760 770 780 SSLCRAVRII SDTTDPNAQL ALVENATAMQ NNINAILGTN VSIPFLSATR RLLITRRVQE 790 800 810 820 830 840 AGAESRSGAT PETVQQLADA ELAKIVSEAN MYNEMAASQR DIANATREAT IREHVLSPVN 850 860 870 880 890 ALANVGMAAA FFRSGGLRSR AFNPAMPTMP GGPAAAGRPM FQAFRGRGHR LNR

References

[1]	"The complete nucleotide sequence of RNA2 of barley mild mosaic virus (BaMMV)." Timpe U., Kuehne T. Eur. J. Plant Pathol. 100:233-241(1994) [Agricola: IND20480706] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75933 Genomic RNA. Translation: CAA53537.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001337. TMV-like_coat. [Graphical view]
Pfam	PF00721. TMV_coat. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POL2_BAMMA

Accession

Primary (citable) accession number: Q65329

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)