Q65243 (DUTP_ASFM2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Ordered Locus Names:	Mal-139
ORF Names:	k1R

Organism

African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV) [Complete proteome]

Taxonomic identifier

10500 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus › African swine fever virus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The viral dUTPase may play a role in lowering the dUTP concentration in natural infections to minimize misincorporation of deoxyuridine into the viral DNA and ensure the fidelity of genome replication By similarity.
Catalytic activity	dUTP + H2O = dUMP + diphosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Homotrimer By similarity.
Subcellular location	Host cytoplasm By similarity.
Miscellaneous	Expressed early and late in the infection By similarity.
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Host cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 162	162	Deoxyuridine 5'-triphosphate nucleotidohydrolase		PRO_0000373135

Sequences

	Sequence		Length	Mass (Da)
	Q65243 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FFD71331454A5737	FASTA	162	17,872
	10 20 30 40 50 60 MATIFFIQPI TEEAEAYYPP SMITNKRKDL GVDVYCCSNL VLQPGLNIIP LHIKVACEHM 70 80 90 100 110 120 GKKCGFKIMA RSSMCTHERL LILANGIGLI DPGYVGELML KIINLGDIPV QIWAKECLVQ 130 140 150 160 LVAQGDHVPD HINILKRNEI FPLFAPTPRG EGRFGSTGKA GL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a 55 kbp region from the right end of the genome of a pathogenic African swine fever virus isolate (Malawi LIL20/1)." Dixon L.K., Twigg S.R.F., Baylis S.A., Vydelingum S., Bristow C., Hammond J.M., Smith G.L. J. Gen. Virol. 75:1655-1684(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"African swine fever virus genomes." Kutish G.F., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71982 Genomic DNA. Translation: CAA50839.1. AY261361 Genomic DNA. No translation available.
3D structure databases
HSSP	HSSP built from PDB template 1VYQ based on UniProtKB Q8II92.
ProteinModelPortal	Q65243.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008180. dUTP_pyroPase. [Graphical view]
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUTP_ASFM2

Accession

Primary (citable) accession number: Q65243

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program