Reviewed, UniProtKB/Swiss-Prot Q65216 (MCE_ASFM2)

Names and origin

Protein names

Recommended name:
    Probable mRNA-capping enzyme
Alternative name(s):
    mRNA guanylyltransferase
    EC=2.7.7.50
    GTP--RNA guanylyltransferase
      Short name=GTase

Gene names

Ordered Locus Names:	Mal-109
ORF Names:	g4R

Organism

African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV)

Taxonomic identifier

10500 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]

Protein attributes

Sequence length	868 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the second reaction in the mRNA cap formation pathway By similarity.
Catalytic activity	GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
Pathway	mRNA processing; mRNA capping.
Sequence similarities	Belongs to the viral GTase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 868	868	Probable mRNA-capping enzyme		PRO_0000373092
Sites
	Active site	282	1	N6-GMP-lysine intermediate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q65216-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4657BBD07E25DC62	FASTA	868	99,937
	10 20 30 40 50 60 MASLENLVAR YQRCFNDQSL KNSTIELEIR FQHINFLLFK TVYEALVAQE IPSTISHSIR 70 80 90 100 110 120 CIKKVHHENH CREKILPSEN LYFKKQPLMF FKFSEPASLG CKVSLAIEQP IRKFILDSSV 130 140 150 160 170 180 LVRLKNRTTF QISDLWKIEL TIVKQLMGSE VSAKLTAFKT LLFDTPEQQT AKNMMTLINP 190 200 210 220 230 240 DDEYLYEIEI EYTGKPESLT AADVIKIKNT VLTLISPNHL MLTAYHQAIE FIASHILSSE 250 260 270 280 290 300 ILLARIKSGK WGLKRLLPQV KSMTKADYMK FYPPVGYYVT DKADGIRGIA VIQDTQIYVV 310 320 330 340 350 360 ADQLYSLGTT GIEPLKPTIL DGEFMPEKKE FYGFDVIMYE GNLLTQQGFE IRIESLNKGI 370 380 390 400 410 420 KVLQAFNIKA EMKPFISLTS ADPNVLLKNF ESVFKKKTRP YAIDGLILVE PGNSYLNTNT 430 440 450 460 470 480 FKWKPTWDNT LDFLVRKCPE SLNVPEYAPK KGFSLYLLFV GISGELFKKL ALNWCPGYTK 490 500 510 520 530 540 LFPVTQRNQN YFPVQFQPSD FPLAFLYYHP DTSSFSDIDG KVLEMRCLKR EVNHVSWEIV 550 560 570 580 590 600 KIREDRQQDL KTGGYFGNDF KTAELTWLNY MDPFSFEELA KGPSGMYFAG AKTGIYRAQT 610 620 630 640 650 660 ALISFIKQEI IQKISHQSWV IDLGIGKGQD LGRYLDAGIR HLVGIDKDQT ALAELIYRKF 670 680 690 700 710 720 SHATTRQHKH ATNIYVLHQD LAEPAKEISE KVHQIYGFPK EGASSIVSNL FIHYLMKNSQ 730 740 750 760 770 780 QVENLAVLCH KLLQPGGMVW FTTMLGERVL ELLHENRIEL NEVWEARENE VVKFAIKRLF 790 800 810 820 830 840 KEDILQETGQ EIGVLLPFSN GDFYNEYLVN TAFLIKIFKH HGFSLVQMQS FKDWIPEFQT 850 860 FSKTLYKILT EADKTWTSLF GFICLRKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a 55 kbp region from the right end of the genome of a pathogenic African swine fever virus isolate (Malawi LIL20/1)." Dixon L.K., Twigg S.R.F., Baylis S.A., Vydelingum S., Bristow C., Hammond J.M., Smith G.L. J. Gen. Virol. 75:1655-1684(1994) [PubMed: 8021596] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"African swine fever virus genomes." Kutish G.F., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71982 Genomic DNA. Translation: CAA50806.1. AY261361 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001339. mRNA_cap_enzyme. IPR004971. Pox_MCEL. [Graphical view]
Pfam	PF01331. mRNA_cap_enzyme. 1 hit. PF03291. Pox_MCEL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCE_ASFM2

Accession

Primary (citable) accession number: Q65216

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)