Reviewed, UniProtKB/Swiss-Prot Q65215 (RPB1_ASFM2)

Names and origin

Protein names

Recommended name:
    Probable DNA-directed RNA polymerase subunit 1 homolog
    EC=2.7.7.6

Gene names

Ordered Locus Names:	Mal-107
ORF Names:	g2L

Organism

African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV)

Taxonomic identifier

10500 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]

Protein attributes

Sequence length	1450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the DNA-dependent RNA polymerase that catalyzes the transcription in the cytoplasm of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase that consists of at least 6 subunits Probable.
Domain	Lacks the typical C-terminal domain (CTD).
Sequence similarities	Belongs to the RNA polymerase beta' chain family.
Sequence caution	The sequence CAA50804.1 differs from that shown. Reason: Frameshift at position 44.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	49 – 1450	1402	Probable DNA-directed RNA polymerase subunit 1 homolog		PRO_5000146089
Experimental info
	Sequence conflict	294	1	Missing in CAA50804. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q65215-1 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 930544E892505596	FASTA	1,450	163,812
	10 20 30 40 50 60 MEAGYAEIAA VQFNIAGDND HKRQGVMEVT ISNLFEGTLP AEGGIYDARM GTTDHHYKCI 70 80 90 100 110 120 TCSHQRKQCM GHPGILQMHA PVLQPLFIAE IRRWLRVICL NCGAPIVDLK RYEHLIRPKR 130 140 150 160 170 180 LVEAASGQTE GKQCYVCKVI HPKIIKDSED YFTFWVDQQG KIDKLYPQII REIFSRVTYD 190 200 210 220 230 240 TVVKLGRSKN SHPEKLVLKA IQIPPISIRP GIRLGIGSGP QSFHDINNVI QYLVRKNLLI 250 260 270 280 290 300 PKDLQIVRGQ KIPLNVDRNL QTIQQLYYNF LLDSVSTTAT QGGTGKRGIV MGARPAPSIM 310 320 330 340 350 360 RRLPRKEGRI RKSLLGSQVW SISRSTICGN SDLHLDEVGY PISFARTLQV AETVQHYNIN 370 380 390 400 410 420 RLMPYFLNGK RQYPGCSRVY KQITQSVHDI EGLKQDFRLE VGDILYRDVV TGDVAFFNRQ 430 440 450 460 470 480 PSLERSSIGV HRIVVLENPK ISTFQMNVSA CAWYNADFDG DQMNLWVPWS VMSRVEAELL 490 500 510 520 530 540 CSVRNWFIST KSSGPVNGQV QDSTVGSFLL TRTNTPMGKN VMNKLHAMGL FQTTQTDPPC 550 560 570 580 590 600 FANYSPTDLL DGKSVVSMLL KQTPINYQRA PTWYSEVYAP YMHYNKQDIS TQIRNGELIE 610 620 630 640 650 660 GVLDKKAVGA GSSGGIYHLI SRRYGPQQAL KMIFATQQLA LNYVRNAGFT VSTADMLLTP 670 680 690 700 710 720 EAHQEVQEII NELLLESEEI NNRLLHGDIM PPIGLTTHDF YEKLQLNALK FPDRILKPIM 730 740 750 760 770 780 NSINPETNGL FQMVATGAKG SNPNMIHIMA GIGQIEINTQ RIQPQFSFGR TLVYYPRFAL 790 800 810 820 830 840 EAQAYGFICN SYIAGLTSPE FIFGEMNGRF DLINKALSTS STGYANRKAI FGLQSCIVDY 850 860 870 880 890 900 YRRVSIDTRL VQQLYGEDGL DARQLETVRF ETIMLSDQEL EDKFKYTGIQ SPLFEEEFSR 910 920 930 940 950 960 LKKDRDKYRQ IFLNIENFNF SQLLTDIRQV PVNVASIVKN ILLSSTSGVL PFDEKSILQK 970 980 990 1000 1010 1020 YTMVKTFCKN LPYVFINNIQ ERLQTPIPVY LKRAASLMRM LIRIELATVK TLNITCEQMS 1030 1040 1050 1060 1070 1080 AILDLIRLQY TQSLINYGEA VGILAAQSVS EPLTQYMLDS HHRSVAGGTN KSGIVRPQEI 1090 1100 1110 1120 1130 1140 FSAKPVEAEQ SSEMLLRLKN PEVETNKTYA QEIANSIELI TFERLILQWH LLYETYSSTK 1150 1160 1170 1180 1190 1200 KNVMYPDFAS DVEWMTDFLE NHPLLQPPED IANWCIRLEL NKTTMILKSI SLESIINSLR 1210 1220 1230 1240 1250 1260 AKHPNSYIMH SVENTASGIP IIIRIYLRES AFRRSTNTRM ATDEKIAVNM VDKLLNSTIR 1270 1280 1290 1300 1310 1320 GIPGIKNANV VKLMRHRVDA QGKLARLDNI YAIKTNGTNI FGAMLDDNID PYTIVSSSIG 1330 1340 1350 1360 1370 1380 DTMELYGIEA ARQKIISEIR TVMGDKGPNH RHLLMYADLM TRTGQVTSLE KAGLNAREPS 1390 1400 1410 1420 1430 1440 NVLLRMALSS PVQVLTDAAV DSAVNPIYGI AAPTLMGSVP RIGTMYSDII MDEKYITENY 1450 KSVDSMIDML

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"African swine fever virus genomes." Kutish G.F., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Nucleotide sequence of a 55 kbp region from the right end of the genome of a pathogenic African swine fever virus isolate (Malawi LIL20/1)." Dixon L.K., Twigg S.R.F., Baylis S.A., Vydelingum S., Bristow C., Hammond J.M., Smith G.L. J. Gen. Virol. 75:1655-1684(1994) [PubMed: 8021596] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-332.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY261361 Genomic DNA. No translation available. X71982 Genomic DNA. Translation: CAA50804.1. Frameshift.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. IPR007073. RNA_pol_Rpb1_7. [Graphical view]
Gene3D	G3DSA:2.40.40.30. RNA_pol_A. 1 hit. G3DSA:3.90.1120.10. RNA_pol_Rpb1_1. 1 hit. G3DSA:3.30.1360.90. RNA_pol_Rpb1_7. 1 hit.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPB1_ASFM2

Accession

Primary (citable) accession number: Q65215

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	June 16, 2009
This is version 27 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)