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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

Ba71V-131

Organism
African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The viral dUTPase may play a role in lowering the dUTP concentration in natural infections to minimize misincorporation of deoxyuridine into the viral DNA and ensure the fidelity of genome replication.

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Mg2+Curated

Kineticsi

  1. KM=1 µM for dUTP

GO - Molecular functioni

  1. dUTP diphosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dUTP metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
Gene namesi
Ordered Locus Names:Ba71V-131
ORF Names:E165R
OrganismiAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Taxonomic identifieri10498 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000624 Componenti: Genome

Subcellular locationi

Host cytoplasm 1 Publication

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 165165Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_0000373133Add
BLAST

Interactioni

Subunit structurei

Homotrimer.

Structurei

3D structure databases

ProteinModelPortaliQ65199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the dUTPase family.Curated

Phylogenomic databases

KOiK01520.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.

Sequencei

Sequence statusi: Complete.

Q65199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATNFFIQPI TQEAEAYYPP SVITNKRKDL GVDVYCCSDL VLQPGLNIVR
60 70 80 90 100
LHIKVACEHM GKKCGFKIMA RSSMCTHERL LILANGIGLI DPGYVGELML
110 120 130 140 150
KIINLGDTPV QIWAKECLVQ LVAQGDHVPD HINILKRNQI FPLFAPTPRG
160
EGRFGSTGEA GIMRT
Length:165
Mass (Da):18,262
Last modified:November 1, 1996 - v1
Checksum:i06633952CDC927E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18466 Genomic DNA. Translation: AAA65359.1.
RefSeqiNP_042823.1. NC_001659.2.

Genome annotation databases

GeneIDi22220359.
KEGGivg:22220359.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18466 Genomic DNA. Translation: AAA65359.1.
RefSeqiNP_042823.1. NC_001659.2.

3D structure databases

ProteinModelPortaliQ65199.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi22220359.
KEGGivg:22220359.

Phylogenomic databases

KOiK01520.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the complete nucleotide sequence of African swine fever virus."
    Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E.
    Virology 208:249-278(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "African swine fever virus dUTPase is a highly specific enzyme required for efficient replication in swine macrophages."
    Oliveros M., Garcia-Escudero R., Alejo A., Vinuela E., Salas M.L., Salas J.
    J. Virol. 73:8934-8943(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDUTP_ASFB7
AccessioniPrimary (citable) accession number: Q65199
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Expressed early and late in the infection.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.