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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

Ba71V-131

Organism
African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The viral dUTPase may play a role in lowering the dUTP concentration in natural infections to minimize misincorporation of deoxyuridine into the viral DNA and ensure the fidelity of genome replication.

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Mg2+Curated

Kineticsi

  1. KM=1 µM for dUTP

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Nucleotide metabolism

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
    Short name:
    dUTPase
    Alternative name(s):
    dUTP pyrophosphatase
    Gene namesi
    Ordered Locus Names:Ba71V-131
    ORF Names:E165R
    OrganismiAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
    Taxonomic identifieri10498 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
    Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000000624 Componenti: Genome

    Subcellular locationi

    • Host cytoplasm 1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Host cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 165165Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_0000373133Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.

    Structurei

    3D structure databases

    ProteinModelPortaliQ65199.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the dUTPase family.Curated

    Phylogenomic databases

    KOiK01520.

    Family and domain databases

    Gene3Di2.70.40.10. 1 hit.
    InterProiIPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    [Graphical view]
    PfamiPF00692. dUTPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51283. SSF51283. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q65199-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MATNFFIQPI TQEAEAYYPP SVITNKRKDL GVDVYCCSDL VLQPGLNIVR
    60 70 80 90 100
    LHIKVACEHM GKKCGFKIMA RSSMCTHERL LILANGIGLI DPGYVGELML
    110 120 130 140 150
    KIINLGDTPV QIWAKECLVQ LVAQGDHVPD HINILKRNQI FPLFAPTPRG
    160
    EGRFGSTGEA GIMRT
    Length:165
    Mass (Da):18,262
    Last modified:November 1, 1996 - v1
    Checksum:i06633952CDC927E5
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U18466 Genomic DNA. Translation: AAA65359.1.
    RefSeqiNP_042823.1. NC_001659.2.

    Genome annotation databases

    GeneIDi22220359.
    KEGGivg:22220359.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U18466 Genomic DNA. Translation: AAA65359.1.
    RefSeqiNP_042823.1. NC_001659.2.

    3D structure databases

    ProteinModelPortaliQ65199.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi22220359.
    KEGGivg:22220359.

    Phylogenomic databases

    KOiK01520.

    Family and domain databases

    Gene3Di2.70.40.10. 1 hit.
    InterProiIPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    [Graphical view]
    PfamiPF00692. dUTPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51283. SSF51283. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Analysis of the complete nucleotide sequence of African swine fever virus."
      Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E.
      Virology 208:249-278(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "African swine fever virus dUTPase is a highly specific enzyme required for efficient replication in swine macrophages."
      Oliveros M., Garcia-Escudero R., Alejo A., Vinuela E., Salas M.L., Salas J.
      J. Virol. 73:8934-8943(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiDUTP_ASFB7
    AccessioniPrimary (citable) accession number: Q65199
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: November 1, 1996
    Last modified: April 1, 2015
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Expressed early and late in the infection.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.