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Q65199

- DUTP_ASFB7

UniProt

Q65199 - DUTP_ASFB7

Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

Ba71V-131

Organism
African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    The viral dUTPase may play a role in lowering the dUTP concentration in natural infections to minimize misincorporation of deoxyuridine into the viral DNA and ensure the fidelity of genome replication.

    Catalytic activityi

    dUTP + H2O = dUMP + diphosphate.

    Cofactori

    Magnesium.Curated

    Kineticsi

    1. KM=1 µM for dUTP

    GO - Molecular functioni

    1. dUTP diphosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. dUTP metabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Nucleotide metabolism

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
    Short name:
    dUTPase
    Alternative name(s):
    dUTP pyrophosphatase
    Gene namesi
    Ordered Locus Names:Ba71V-131
    ORF Names:E165R
    OrganismiAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
    Taxonomic identifieri10498 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
    Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000000624: Genome

    Subcellular locationi

    Host cytoplasm 1 Publication

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 165165Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_0000373133Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.

    Structurei

    3D structure databases

    ProteinModelPortaliQ65199.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the dUTPase family.Curated

    Family and domain databases

    Gene3Di2.70.40.10. 1 hit.
    InterProiIPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    [Graphical view]
    PfamiPF00692. dUTPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51283. SSF51283. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q65199-1 [UniParc]FASTAAdd to Basket

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    MATNFFIQPI TQEAEAYYPP SVITNKRKDL GVDVYCCSDL VLQPGLNIVR    50
    LHIKVACEHM GKKCGFKIMA RSSMCTHERL LILANGIGLI DPGYVGELML 100
    KIINLGDTPV QIWAKECLVQ LVAQGDHVPD HINILKRNQI FPLFAPTPRG 150
    EGRFGSTGEA GIMRT 165
    Length:165
    Mass (Da):18,262
    Last modified:November 1, 1996 - v1
    Checksum:i06633952CDC927E5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18466 Genomic DNA. Translation: AAA65359.1.
    RefSeqiNP_042823.1. NC_001659.1.

    Genome annotation databases

    GeneIDi1488894.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18466 Genomic DNA. Translation: AAA65359.1 .
    RefSeqi NP_042823.1. NC_001659.1.

    3D structure databases

    ProteinModelPortali Q65199.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1488894.

    Family and domain databases

    Gene3Di 2.70.40.10. 1 hit.
    InterProi IPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    [Graphical view ]
    Pfami PF00692. dUTPase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51283. SSF51283. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the complete nucleotide sequence of African swine fever virus."
      Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E.
      Virology 208:249-278(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "African swine fever virus dUTPase is a highly specific enzyme required for efficient replication in swine macrophages."
      Oliveros M., Garcia-Escudero R., Alejo A., Vinuela E., Salas M.L., Salas J.
      J. Virol. 73:8934-8943(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiDUTP_ASFB7
    AccessioniPrimary (citable) accession number: Q65199
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Expressed early and late in the infection.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3