Q65199 (DUTP_ASFB7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Ordered Locus Names:	Ba71V-131
ORF Names:	E165R

Organism

African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV) [Reference proteome]

Taxonomic identifier

10498 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus › African swine fever virus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The viral dUTPase may play a role in lowering the dUTP concentration in natural infections to minimize misincorporation of deoxyuridine into the viral DNA and ensure the fidelity of genome replication.
Catalytic activity	dUTP + H2O = dUMP + diphosphate.
Cofactor	Magnesium Probable.
Subunit structure	Homotrimer.
Subcellular location	Host cytoplasm Ref.2.
Miscellaneous	Expressed early and late in the infection.
Sequence similarities	Belongs to the dUTPase family.
Biophysicochemical properties	Kinetic parameters: KM=1 µM for dUTP

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Host cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	Deoxyuridine 5'-triphosphate nucleotidohydrolase		PRO_0000373133

Sequences

	Sequence		Length	Mass (Da)
	Q65199 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 06633952CDC927E5	FASTA	165	18,262
	10 20 30 40 50 60 MATNFFIQPI TQEAEAYYPP SVITNKRKDL GVDVYCCSDL VLQPGLNIVR LHIKVACEHM 70 80 90 100 110 120 GKKCGFKIMA RSSMCTHERL LILANGIGLI DPGYVGELML KIINLGDTPV QIWAKECLVQ 130 140 150 160 LVAQGDHVPD HINILKRNQI FPLFAPTPRG EGRFGSTGEA GIMRT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the complete nucleotide sequence of African swine fever virus." Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E. Virology 208:249-278(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"African swine fever virus dUTPase is a highly specific enzyme required for efficient replication in swine macrophages." Oliveros M., Garcia-Escudero R., Alejo A., Vinuela E., Salas M.L., Salas J. J. Virol. 73:8934-8943(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18466 Genomic DNA. Translation: AAA65359.1.
RefSeq	NP_042823.1. NC_001659.1.
3D structure databases
HSSP	HSSP built from PDB template 1VYQ based on UniProtKB Q8II92.
ProteinModelPortal	Q65199.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1488894.
Family and domain databases
InterPro	IPR008180. dUTP_pyroPase. [Graphical view]
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUTP_ASFB7

Accession

Primary (citable) accession number: Q65199

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program