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Q65199 (DUTP_ASFB7) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase

Short name=dUTPase
EC=3.6.1.23
Alternative name(s):
dUTP pyrophosphatase
Gene names
Ordered Locus Names:Ba71V-131
ORF Names:E165R
OrganismAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV) [Reference proteome]
Taxonomic identifier10498 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length165 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The viral dUTPase may play a role in lowering the dUTP concentration in natural infections to minimize misincorporation of deoxyuridine into the viral DNA and ensure the fidelity of genome replication.

Catalytic activity

dUTP + H2O = dUMP + diphosphate.

Cofactor

Magnesium Probable.

Subunit structure

Homotrimer.

Subcellular location

Host cytoplasm Ref.2.

Miscellaneous

Expressed early and late in the infection.

Sequence similarities

Belongs to the dUTPase family.

Biophysicochemical properties

Kinetic parameters:

KM=1 µM for dUTP

Ontologies

Keywords
   Biological processNucleotide metabolism
   Cellular componentHost cytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdUTP metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondUTP diphosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 165165Deoxyuridine 5'-triphosphate nucleotidohydrolase
PRO_0000373133

Sequences

Sequence LengthMass (Da)Tools
Q65199 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 06633952CDC927E5

FASTA16518,262
        10         20         30         40         50         60 
MATNFFIQPI TQEAEAYYPP SVITNKRKDL GVDVYCCSDL VLQPGLNIVR LHIKVACEHM 

        70         80         90        100        110        120 
GKKCGFKIMA RSSMCTHERL LILANGIGLI DPGYVGELML KIINLGDTPV QIWAKECLVQ 

       130        140        150        160 
LVAQGDHVPD HINILKRNQI FPLFAPTPRG EGRFGSTGEA GIMRT 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the complete nucleotide sequence of African swine fever virus."
Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E.
Virology 208:249-278(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"African swine fever virus dUTPase is a highly specific enzyme required for efficient replication in swine macrophages."
Oliveros M., Garcia-Escudero R., Alejo A., Vinuela E., Salas M.L., Salas J.
J. Virol. 73:8934-8943(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18466 Genomic DNA. Translation: AAA65359.1.
RefSeqNP_042823.1. NC_001659.1.

3D structure databases

ProteinModelPortalQ65199.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1488894.

Family and domain databases

Gene3D2.70.40.10. 1 hit.
InterProIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
[Graphical view]
PfamPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMSSF51283. SSF51283. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDUTP_ASFB7
AccessionPrimary (citable) accession number: Q65199
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families