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Q65164

- TPRT_ASFB7

UniProt

Q65164 - TPRT_ASFB7

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Protein

Trans-prenyltransferase

Gene
Ba71V-074, B318L
Organism
African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Trans-prenyltransferase that catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with different allylic diphosphates, such as dimethylallyl diphosphate (DMAPP), geranyl diphosphate (GPP), farnesyl diphosphate (FPP) and geranylgeranyl diphosphate (GGPP), farnesyl diphosphate being the best allylic substrate.

Catalytic activityi

Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate.
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.
Geranylgeranyl diphosphate + isopentenyl diphosphate = diphosphate + geranylfarnesyl diphosphate.

Cofactori

Binds 3 magnesium ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851Isopentenyl diphosphate By similarity
Binding sitei88 – 881Isopentenyl diphosphate By similarity
Binding sitei122 – 1221Isopentenyl diphosphate By similarity
Metal bindingi129 – 1291Magnesium 1 By similarity
Metal bindingi129 – 1291Magnesium 2 By similarity
Metal bindingi135 – 1351Magnesium 1 By similarity
Metal bindingi135 – 1351Magnesium 2 By similarity
Binding sitei140 – 1401Dimethylallyl diphosphate By similarity
Binding sitei141 – 1411Isopentenyl diphosphate By similarity
Binding sitei216 – 2161Dimethylallyl diphosphate By similarity
Binding sitei217 – 2171Dimethylallyl diphosphate By similarity
Binding sitei254 – 2541Dimethylallyl diphosphate By similarity
Metal bindingi257 – 2571Magnesium 3 By similarity

GO - Molecular functioni

  1. dimethylallyltranstransferase activity Source: UniProtKB-EC
  2. farnesyltranstransferase activity Source: UniProtKB-EC
  3. geranyltranstransferase activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. farnesyl diphosphate biosynthetic process Source: UniProtKB-UniPathway
  2. geranyl diphosphate biosynthetic process Source: UniProtKB-UniPathway
  3. geranylgeranyl diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00259; UER00368.
UPA00260; UER00369.
UPA00389; UER00564.

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-prenyltransferase (EC:2.5.1.-)
Alternative name(s):
(2E,6E)-farnesyl diphosphate synthase
Dimethylallyltranstransferase (EC:2.5.1.1)
Farnesyl diphosphate synthase
Farnesyltranstransferase (EC:2.5.1.29)
Geranyltranstransferase (EC:2.5.1.10)
Polyprenyl-diphosphate synthase
Gene namesi
Ordered Locus Names:Ba71V-074
ORF Names:B318L
OrganismiAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Taxonomic identifieri10498 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000624: Genome

Subcellular locationi

Host endoplasmic reticulum. Host membrane; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. host cell endoplasmic reticulum Source: UniProtKB-SubCell
  2. host cell membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Trans-prenyltransferasePRO_0000373156Add
BLAST

Expressioni

Inductioni

Expressed late in the infection cycle.

Keywords - Developmental stagei

Late protein

Structurei

3D structure databases

ProteinModelPortaliQ65164.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR000092. Polyprenyl_synt.
IPR017446. Polyprenyl_synth-rel.
IPR008949. Terpenoid_synth.
[Graphical view]
PANTHERiPTHR12001. PTHR12001. 1 hit.
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65164-1 [UniParc]FASTAAdd to Basket

« Hide

MLHLIYISII VVLIIILISY TRKPKYFRIT APRSVALFHG IHPLNPKNYK    50
TFSKEFETIL NNAIEDGDFK GQLTEPCSYA LRGGKYIRPI ILMEIVRACQ 100
LQHSFGAPIY PAEAALAVEY FHVASLIIDD MPSFDNDVKR RNKDTVWARF 150
GVAKAQMSAL ALTMQGFQNI CRQIDWIKEH CPRFPDPNQL GALLCTFVSH 200
SLNSAGSGQL VDTPEKTIPF FKIAFIMGWV LGTGTIEDIG MIERAAHCFG 250
HAFQLADDIK DHDTDTGWNY AKIHGKQKTF DDVAQSLQEC KKILHGKKIF 300
TSIWNEIFQK VINVALGT 318
Length:318
Mass (Da):35,905
Last modified:November 1, 1996 - v1
Checksum:i5279923BF0422CB0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18466 Genomic DNA. Translation: AAA65304.1.
RefSeqiNP_042768.1. NC_001659.1.

Genome annotation databases

GeneIDi1488839.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18466 Genomic DNA. Translation: AAA65304.1 .
RefSeqi NP_042768.1. NC_001659.1.

3D structure databases

ProteinModelPortali Q65164.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1488839.

Enzyme and pathway databases

UniPathwayi UPA00259 ; UER00368 .
UPA00260 ; UER00369 .
UPA00389 ; UER00564 .

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
InterProi IPR000092. Polyprenyl_synt.
IPR017446. Polyprenyl_synth-rel.
IPR008949. Terpenoid_synth.
[Graphical view ]
PANTHERi PTHR12001. PTHR12001. 1 hit.
Pfami PF00348. polyprenyl_synt. 1 hit.
[Graphical view ]
SUPFAMi SSF48576. SSF48576. 1 hit.
PROSITEi PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the complete nucleotide sequence of African swine fever virus."
    Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E.
    Virology 208:249-278(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTPRT_ASFB7
AccessioniPrimary (citable) accession number: Q65164
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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