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Protein

Trans-prenyltransferase

Gene

Ba71V-074

Organism
African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Trans-prenyltransferase that catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with different allylic diphosphates, such as dimethylallyl diphosphate (DMAPP), geranyl diphosphate (GPP), farnesyl diphosphate (FPP) and geranylgeranyl diphosphate (GGPP), farnesyl diphosphate being the best allylic substrate.

Catalytic activityi

Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate.
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.
Geranylgeranyl diphosphate + isopentenyl diphosphate = diphosphate + geranylfarnesyl diphosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Pathwayi: farnesyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Trans-prenyltransferase (Ba71V-074)
This subpathway is part of the pathway farnesyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate, the pathway farnesyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: geranyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Trans-prenyltransferase (Ba71V-074)
This subpathway is part of the pathway geranyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate, the pathway geranyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: geranylgeranyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Trans-prenyltransferase (Ba71V-074)
This subpathway is part of the pathway geranylgeranyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate, the pathway geranylgeranyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei85Isopentenyl diphosphateBy similarity1
Binding sitei88Isopentenyl diphosphateBy similarity1
Binding sitei122Isopentenyl diphosphateBy similarity1
Metal bindingi129Magnesium 1By similarity1
Metal bindingi129Magnesium 2By similarity1
Metal bindingi135Magnesium 1By similarity1
Metal bindingi135Magnesium 2By similarity1
Binding sitei140Dimethylallyl diphosphateBy similarity1
Binding sitei141Isopentenyl diphosphateBy similarity1
Binding sitei216Dimethylallyl diphosphateBy similarity1
Binding sitei217Dimethylallyl diphosphateBy similarity1
Binding sitei254Dimethylallyl diphosphateBy similarity1
Metal bindingi257Magnesium 3By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processIsoprene biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00259; UER00368.
UPA00260; UER00369.
UPA00389; UER00564.

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-prenyltransferase (EC:2.5.1.-)
Alternative name(s):
(2E,6E)-farnesyl diphosphate synthase
Dimethylallyltranstransferase (EC:2.5.1.1)
Farnesyl diphosphate synthase
Farnesyltranstransferase (EC:2.5.1.29)
Geranyltranstransferase (EC:2.5.1.10)
Polyprenyl-diphosphate synthase
Gene namesi
Ordered Locus Names:Ba71V-074
ORF Names:B318L
OrganismiAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Taxonomic identifieri10498 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000000624 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Host endoplasmic reticulum, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003731561 – 318Trans-prenyltransferaseAdd BLAST318

Expressioni

Inductioni

Expressed late in the infection cycle.

Keywords - Developmental stagei

Late protein

Structurei

3D structure databases

ProteinModelPortaliQ65164.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG090000FW.

Family and domain databases

Gene3Di1.10.600.10. 2 hits.
InterProiView protein in InterPro
IPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
PfamiView protein in Pfam
PF00348. polyprenyl_synt. 1 hit.
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiView protein in PROSITE
PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q65164-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHLIYISII VVLIIILISY TRKPKYFRIT APRSVALFHG IHPLNPKNYK
60 70 80 90 100
TFSKEFETIL NNAIEDGDFK GQLTEPCSYA LRGGKYIRPI ILMEIVRACQ
110 120 130 140 150
LQHSFGAPIY PAEAALAVEY FHVASLIIDD MPSFDNDVKR RNKDTVWARF
160 170 180 190 200
GVAKAQMSAL ALTMQGFQNI CRQIDWIKEH CPRFPDPNQL GALLCTFVSH
210 220 230 240 250
SLNSAGSGQL VDTPEKTIPF FKIAFIMGWV LGTGTIEDIG MIERAAHCFG
260 270 280 290 300
HAFQLADDIK DHDTDTGWNY AKIHGKQKTF DDVAQSLQEC KKILHGKKIF
310
TSIWNEIFQK VINVALGT
Length:318
Mass (Da):35,905
Last modified:November 1, 1996 - v1
Checksum:i5279923BF0422CB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18466 Genomic DNA. Translation: AAA65304.1.
RefSeqiNP_042768.1. NC_001659.2.

Genome annotation databases

GeneIDi22220304.
KEGGivg:22220304.

Similar proteinsi

Entry informationi

Entry nameiTPRT_ASFB7
AccessioniPrimary (citable) accession number: Q65164
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families