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Q65164

- TPRT_ASFB7

UniProt

Q65164 - TPRT_ASFB7

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Protein

Trans-prenyltransferase

Gene

Ba71V-074

Organism
African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Trans-prenyltransferase that catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with different allylic diphosphates, such as dimethylallyl diphosphate (DMAPP), geranyl diphosphate (GPP), farnesyl diphosphate (FPP) and geranylgeranyl diphosphate (GGPP), farnesyl diphosphate being the best allylic substrate.

Catalytic activityi

Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate.
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.
Geranylgeranyl diphosphate + isopentenyl diphosphate = diphosphate + geranylfarnesyl diphosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg(2+) ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851Isopentenyl diphosphateBy similarity
Binding sitei88 – 881Isopentenyl diphosphateBy similarity
Binding sitei122 – 1221Isopentenyl diphosphateBy similarity
Metal bindingi129 – 1291Magnesium 1By similarity
Metal bindingi129 – 1291Magnesium 2By similarity
Metal bindingi135 – 1351Magnesium 1By similarity
Metal bindingi135 – 1351Magnesium 2By similarity
Binding sitei140 – 1401Dimethylallyl diphosphateBy similarity
Binding sitei141 – 1411Isopentenyl diphosphateBy similarity
Binding sitei216 – 2161Dimethylallyl diphosphateBy similarity
Binding sitei217 – 2171Dimethylallyl diphosphateBy similarity
Binding sitei254 – 2541Dimethylallyl diphosphateBy similarity
Metal bindingi257 – 2571Magnesium 3By similarity

GO - Molecular functioni

  1. dimethylallyltranstransferase activity Source: UniProtKB-EC
  2. farnesyltranstransferase activity Source: UniProtKB-EC
  3. geranyltranstransferase activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. farnesyl diphosphate biosynthetic process Source: UniProtKB-UniPathway
  2. geranyl diphosphate biosynthetic process Source: UniProtKB-UniPathway
  3. geranylgeranyl diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00259; UER00368.
UPA00260; UER00369.
UPA00389; UER00564.

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-prenyltransferase (EC:2.5.1.-)
Alternative name(s):
(2E,6E)-farnesyl diphosphate synthase
Dimethylallyltranstransferase (EC:2.5.1.1)
Farnesyl diphosphate synthase
Farnesyltranstransferase (EC:2.5.1.29)
Geranyltranstransferase (EC:2.5.1.10)
Polyprenyl-diphosphate synthase
Gene namesi
Ordered Locus Names:Ba71V-074
ORF Names:B318L
OrganismiAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Taxonomic identifieri10498 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000624: Genome

Subcellular locationi

Host endoplasmic reticulum 1 Publication. Host membrane 1 Publication; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell endoplasmic reticulum Source: UniProtKB-KW
  2. host cell membrane Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Trans-prenyltransferasePRO_0000373156Add
BLAST

Expressioni

Inductioni

Expressed late in the infection cycle.

Keywords - Developmental stagei

Late protein

Structurei

3D structure databases

ProteinModelPortaliQ65164.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR000092. Polyprenyl_synt.
IPR017446. Polyprenyl_synth-rel.
IPR008949. Terpenoid_synth.
[Graphical view]
PANTHERiPTHR12001. PTHR12001. 1 hit.
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65164-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLHLIYISII VVLIIILISY TRKPKYFRIT APRSVALFHG IHPLNPKNYK
60 70 80 90 100
TFSKEFETIL NNAIEDGDFK GQLTEPCSYA LRGGKYIRPI ILMEIVRACQ
110 120 130 140 150
LQHSFGAPIY PAEAALAVEY FHVASLIIDD MPSFDNDVKR RNKDTVWARF
160 170 180 190 200
GVAKAQMSAL ALTMQGFQNI CRQIDWIKEH CPRFPDPNQL GALLCTFVSH
210 220 230 240 250
SLNSAGSGQL VDTPEKTIPF FKIAFIMGWV LGTGTIEDIG MIERAAHCFG
260 270 280 290 300
HAFQLADDIK DHDTDTGWNY AKIHGKQKTF DDVAQSLQEC KKILHGKKIF
310
TSIWNEIFQK VINVALGT
Length:318
Mass (Da):35,905
Last modified:November 1, 1996 - v1
Checksum:i5279923BF0422CB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18466 Genomic DNA. Translation: AAA65304.1.
RefSeqiNP_042768.1. NC_001659.1.

Genome annotation databases

GeneIDi1488839.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18466 Genomic DNA. Translation: AAA65304.1 .
RefSeqi NP_042768.1. NC_001659.1.

3D structure databases

ProteinModelPortali Q65164.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1488839.

Enzyme and pathway databases

UniPathwayi UPA00259 ; UER00368 .
UPA00260 ; UER00369 .
UPA00389 ; UER00564 .

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
InterProi IPR000092. Polyprenyl_synt.
IPR017446. Polyprenyl_synth-rel.
IPR008949. Terpenoid_synth.
[Graphical view ]
PANTHERi PTHR12001. PTHR12001. 1 hit.
Pfami PF00348. polyprenyl_synt. 1 hit.
[Graphical view ]
SUPFAMi SSF48576. SSF48576. 1 hit.
PROSITEi PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the complete nucleotide sequence of African swine fever virus."
    Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E.
    Virology 208:249-278(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTPRT_ASFB7
AccessioniPrimary (citable) accession number: Q65164
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3