FAD-linked sulfhydryl oxidase - African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)

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Reviewed, UniProtKB/Swiss-Prot Q65163 (FLSO_ASFB7)

Last modified November 3, 2009. Version 47. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    FAD-linked sulfhydryl oxidase
    EC=1.8.3.2
Alternative name(s):
    p14

Gene names

Ordered Locus Names:	Ba71V-073
ORF Names:	B119L, 9GL

Organism

African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)

Taxonomic identifier

10498 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]

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Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation By similarity. Involved in virion maturation.
Catalytic activity	4 R'C(R)SH + O₂ = 2 R'C(R)S-S(R)CR' + 2 H₂O.
Cofactor	FAD By similarity.
Subunit structure	Interacts with A151R. Ref.3
Subcellular location	Host cytoplasm. Ref.3
Induction	Expressed late in the infection cycle.
Sequence similarities	Belongs to the asfivirus B119L family. Contains 1 ERV/ALR sulfhydryl oxidase domain.

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Ontologies

Keywords
Biological process	Virulence
Cellular component	Host cytoplasm
Developmental stage	Late protein
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Virus reference strain
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiol oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 119

119

FAD-linked sulfhydryl oxidase

PRO_0000208551

Regions

Domain

1 – 97

ERV/ALR sulfhydryl oxidase

Amino acid modifications

Disulfide bond

44 ↔ 47

Redox-active Potential

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Sequences

Sequence

Length

Mass (Da)

Tools

Q65163-1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: A1EE2F073ABF9726
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FASTA

119

14,378

        10         20         30         40         50         60 
MLHWGPKYWR SLHLYAIFFS DAPSWKEKYE AIQWILNFIE SLPCTRCQHH AFSYLTKNPL 

        70         80         90        100        110 
TLNNSEDFQY WTFAFHNNVN NRLNKKIISW SEYKNIYEQS ILKTIEYGKT DFIGAWSSL

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the complete nucleotide sequence of African swine fever virus." Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E. Virology 208:249-278(1995) [PubMed: 11831707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"An African swine fever virus ERV1-ALR homologue, 9GL, affects virion maturation and viral growth in macrophages and viral virulence in swine." Lewis T., Zsak L., Burrage T.G., Lu Z., Kutish G.F., Neilan J.G., Rock D.L. J. Virol. 74:1275-1285(2000) [PubMed: 10627538] [Abstract] Cited for: CHARACTERIZATION.
[3]	"African swine fever virus pB119L protein is a flavin adenine dinucleotide-linked sulfhydryl oxidase." Rodriguez I., Redrejo-Rodriguez M., Rodriguez J.M., Alejo A., Salas J., Salas M.L. J. Virol. 80:3157-3166(2006) [PubMed: 16537584] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH A151R.

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Cross-references

Sequence databases

U18466 Genomic DNA. Translation: AAA65303.1.

RefSeq

NP_042767.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3GWL	X-ray	2.10	A/B	1-103	[»]

ModBase

Search...

Genome annotation databases

GeneID

1488838.

Family and domain databases

InterPro

IPR017905. ERV/ALR_sulphydryl_oxidase.
IPR006863. Evr1_Alr.
[Graphical view]

Gene3D

G3DSA:1.20.120.310. Evr1_Alr. 1 hit.

Pfam

PF04777. Evr1_Alr. 1 hit.
[Graphical view]

PROSITE

PS51324. ERV_ALR. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

FLSO_ASFB7

Accession

Primary (citable) accession number: Q65163

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families