Q64XW8 (LPXD_BACFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	BF0907

Organism

Bacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]

Taxonomic identifier

295405 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000059644
Sites
	Active site	240	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64XW8 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: D5CBFFE15F51953F	FASTA	346	37,123
	10 20 30 40 50 60 MEFSAKQIAA FIQGEIIGDE NATVHTFAKI EEGIPGAISF LSNPKYTPYI YETKASIVLV 70 80 90 100 110 120 NKDFTPEQEV KATLIKVDNA YESLAKLLNL YEMSKPKRTG IDERAYVAET AKIGKDVYIA 130 140 150 160 170 180 PFACIGDHAE IGDNTVIHPH ATVGGGAKIG SNCILYANST VYHDCRVGNN CILHAGCVIG 190 200 210 220 230 240 ADGFGFAPTP QGYEKIPQIG IVILEDNVEV GANTCIDRAT MGATVIHSGV KLDNLVQIAH 250 260 270 280 290 300 NDEIGSHTVM AAQVGIAGST KVGEWCMFGG QVGIAGHLKI GNQVNLGAQS GVPGNIKSGS 310 320 330 340 QLIGTPPMEL KQFFKASIVQ KSLPEMQIEL RNLRKEIEEL KQQLNK

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006841 Genomic DNA. Translation: BAD47658.1.
RefSeq	YP_098192.1. NC_006347.1.
3D structure databases
ProteinModelPortal	Q64XW8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3082878.
GenomeReviews	Gene locus BF0907 in contig AP006841_GR.
KEGG	bfr:BF0907.
NMPDR	fig|295405.3.peg.811.
PATRIC	21047570. VBIBacFra17906_0910.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG469615.
OMA	SYPAKIM.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	BFRA295405:BF0907-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_BACFR

Accession

Primary (citable) accession number: Q64XW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program