Reviewed, UniProtKB/Swiss-Prot Q64XV2 (GLAB_BACFR)

Names and origin

Protein names

Recommended name:
    Alpha-1,3-galactosidase B
    EC=3.2.1.n1
    EC=3.2.1.n2
Alternative name(s):
    Exo-alpha-galactosidase B
    EC=3.2.1.22

Gene names

Name:	glaB
Ordered Locus Names:	BF0923

Organism

Bacteroides fragilis [Complete proteome] [HAMAP]

Taxonomic identifier

817 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Alpha-galactosidase. Removes both branched alpha-1,3-linked galactose residues of blood group B antigens and linear alpha-1,3-linked galactose structures By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing linear alpha-1,3-linked galactose residues. Hydrolysis of terminal, non-reducing branched alpha-1,3-linked galactose residues. Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.
Sequence similarities	Belongs to the glycosyl hydrolase 110 family. B subfamily. Contains 3 PbH1 repeats.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 595	573	Alpha-1,3-galactosidase B		PRO_0000348477
Regions
	Repeat	432 – 454	23	PbH1 1
	Repeat	455 – 477	23	PbH1 2
	Repeat	488 – 541	54	PbH1 3

Sequences

	Sequence		Length	Mass (Da)
	Q64XV2-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 9B80163520E631B7	FASTA	595	66,982
	10 20 30 40 50 60 MKTILLFALS LLLSLSVSDV CAQERVYDIS QFGLKANSKK NASPVVRKAI AKIKAECRDG 70 80 90 100 110 120 EKVILRFPAG RYNFHEAGST VREYYISNHD QDNPKKVGIA LEDMKNLTID GQGSEFVFYG 130 140 150 160 170 180 RMIPVSLLRS ENCVLKNFSI DFEQPHIAQV QVVENDPEKG ITFEPAPWVD YRISKDSVFE 190 200 210 220 230 240 GLGEGWVMRY SWGIAFDGKT KHVVYNTSDI GCPTKGAFEV APRRICSPKW KDARLVPGTV 250 260 270 280 290 300 VAMRGWGRPT PGIFMSHDVN TSLLDVKVHY AEGMGLLAQL CEDITLDGFG VCLKGNNDPR 310 320 330 340 350 360 YFTTQADATH FSGCKGKIVS KNGLYEGMMD DAINVHGTYL KVIKRVDDHT LIGRYMHDQS 370 380 390 400 410 420 WGFEWGRPGD DVQFVRSETM ELIGKQNQIT AIRPYDKGEI QGAREFSITF KEAIDPAINE 430 440 450 460 470 480 KSGFGIENLT WTPEVLFAGN TIRNNRARGT LFSTPKKTVV EDNLFDHTSG TAILLCGDCN 490 500 510 520 530 540 GWFETGACRD VTIRRNRFIN ALTNMFQFTN AVISIYPEIP NLKDQQKYFH GGKDGGIVIE 550 560 570 580 590 DNEFDTFDAP ILYAKSVDGL IFRNNVIKTN TEFKPFHWNK DRFLLERVTN VKISE

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006841 Genomic DNA. Translation: BAD47674.1.
RefSeq	YP_098208.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3083038.
GenomeReviews	Gene locus BF0923 in contig AP006841_GR.
KEGG	bfr:BF0923.
NMPDR	fig|295405.3.peg.114.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q64XV2.
OMA	KNFSIDF.
Enzyme and pathway databases
BioCyc	BFRA295405:BF0923-MON.
Family and domain databases
InterPro	IPR006626. PbH1. [Graphical view]
SMART	SM00710. PbH1. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLAB_BACFR

Accession

Primary (citable) accession number: Q64XV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)