Reviewed, UniProtKB/Swiss-Prot Q64WS3 (GCST_BACFR)

Names and origin

Protein names

Recommended name:
    Aminomethyltransferase
    EC=2.1.2.10
Alternative name(s):
    Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	BF1303

Organism

Bacteroides fragilis [Complete proteome] [HAMAP]

Taxonomic identifier

817 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity. HAMAP MF_00259
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: HAMAP transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Aminomethyltransferase HAMAP MF_00259		PRO_0000122539

Sequences

	Sequence		Length	Mass (Da)
	Q64WS3-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: E5E9D8FE6A152D8C	FASTA	361	39,870
	10 20 30 40 50 60 MKTTPFTEKH IALGAKMHEF AGYNMPIEYS GIIDEHLTVC NGVGVFDVSH MGEFWVKGPH 70 80 90 100 110 120 ALDFLQKVTS NNVAALVPGK IQYTCFPNED GGIVDDLLVY QYEPEKYLLV VNASNIEKDW 130 140 150 160 170 180 NWCISHNTEG AELENSSDNM AQLAVQGPKA IQALQKLTDI NLADIPYYTF KVGEFAGEKN 190 200 210 220 230 240 VIISNTGYTG AGGFELYFYP DAAMKIWDAV FEAGAEFGIK PIGLGARDTL RLEMGFCLYG 250 260 270 280 290 300 NDLDDTTSPI EAGLGWITKF VDGKNFTNRS MLEKQKAEGT VRKLVGFEMI DRGIPRHGYE 310 320 330 340 350 360 LTTAEGDKIG VVTSGTMSPI RKIGIGMGYV KPEYSKIGTE ICIDMRGRKL KAVVVKPPFR K

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006841 Genomic DNA. Translation: BAD48053.1.
RefSeq	YP_098587.1.
3D structure databases
SMR	Q64WS3. Positions 1-359.
ModBase	Search...
Genome annotation databases
GeneID	3082751.
GenomeReviews	Gene locus BF1303 in contig AP006841_GR.
KEGG	bfr:BF1303.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG299834.
OMA	KALYGGM.
PhylomeDB	Q64WS3.
Enzyme and pathway databases
BioCyc	BFRA295405:BF1303-MONOMER.
BRENDA	2.1.2.10. 868.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. [Graphical view]
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. gcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_BACFR

Accession

Primary (citable) accession number: Q64WS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	October 25, 2004
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)