Q64VP2 (SYT_BACFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	BF1687

Organism

Bacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]

Taxonomic identifier

295405 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	646 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 646	646	Threonine--tRNA ligase HAMAP MF_00184		PRO_0000100936
Regions
	Region	242 – 541	300	Catalytic HAMAP MF_00184
Sites
	Metal binding	337	1	Zinc; catalytic By similarity
	Metal binding	388	1	Zinc; catalytic By similarity
	Metal binding	518	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64VP2 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 22C3B3A70B4B73EC	FASTA	646	74,406
	10 20 30 40 50 60 MIKITFPDGS VREYNEGVNG LQIAESISSR LAQDVLACGV NGEIYDLGRP INEDASVVLY 70 80 90 100 110 120 KWEDEQGKHA FWHTSAHLLA EALQELYPGI QFGIGPAIEN GFYYDVDPGE AVIKEADLPA 130 140 150 160 170 180 IEAKMAELVA KKEAVVRRDI AKGDALKMFG DRGETYKCEL ISELEDGHIT TYTQGDFTDL 190 200 210 220 230 240 CRGPHLMTTA PIKAIKLTSV AGAYWRGHED RKMLTRIYGI TFPKKKMLDE YLALMEEAKK 250 260 270 280 290 300 RDHRKIGKEM QLFMFSDTVG KGLPMWLPKG TALRLRLQDF LRRIQTRYDY QEVITPPIGN 310 320 330 340 350 360 KLLYVTSGHY AKYGKDAFQP IHTPEEGEEY FLKPMNCPHH CEIYKNFPRS YKDLPLRIAE 370 380 390 400 410 420 FGTVCRYEQS GELHGLTRVR SFTQDDAHIF CRPDQVKGEF LRVMDIISIV FRSMDFDNFE 430 440 450 460 470 480 AQISLRDKVN REKYIGSDEN WEKAEQAIIE ACEEKGLKAK IEYGEAAFYG PKLDFMVKDA 490 500 510 520 530 540 IGRRWQLGTI QVDYNLPERF ELEYMGSDNQ KHRPVMIHRA PFGSMERFVA VLIEHTAGKF 550 560 570 580 590 600 PLWLTPEQVV ILPISEKFNE YAEKVKTYLK MKEIRAIVDD RNEKIGRKIR DNEMKRIPYM 610 620 630 640 LIVGEKEAEN GEVSVRRQGE GDKGTMKFEE FGEILNEEVQ NMINKW

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006841 Genomic DNA. Translation: BAD48434.1.
RefSeq	YP_098968.1. NC_006347.1.
3D structure databases
ProteinModelPortal	Q64VP2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3081309.
GenomeReviews	Gene locus BF1687 in contig AP006841_GR.
KEGG	bfr:BF1687.
PATRIC	21049043. VBIBacFra17906_1639.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG352811.
OMA	GRKWQLG.
PhylomeDB	Q64VP2.
ProtClustDB	PRK00413.
Enzyme and pathway databases
BioCyc	BFRA295405:BF1687-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BACFR

Accession

Primary (citable) accession number: Q64VP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program