Q64UB9 (PANB_BACFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase
EC=2.1.2.11

Alternative name(s):
Ketopantoate hydroxymethyltransferase
Short name=KPHMT

Gene names

Name:	panB
Ordered Locus Names:	BF2163

Organism

Bacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]

Taxonomic identifier

295405 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity. HAMAP MF_00156
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00156
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity. HAMAP MF_00156
Subcellular location	Cytoplasm Potential HAMAP MF_00156.
Sequence similarities	Belongs to the PanB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	3-methyl-2-oxobutanoate hydroxymethyltransferase HAMAP MF_00156		PRO_0000184812
Regions
	Region	53 – 54	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	191	1	Proton acceptor By similarity
	Metal binding	53	1	Magnesium By similarity
	Metal binding	92	1	Magnesium By similarity
	Metal binding	124	1	Magnesium By similarity
	Binding site	92	1	Alpha-ketoisovalerate By similarity
	Binding site	122	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64UB9 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: F7EABB8AF3842932	FASTA	273	29,807
	10 20 30 40 50 60 MAGYISDDTR KVTTHRLIEM KQRGEKISML TSYDYTMAQI VDGAGIDVIL VGDSASNVMA 70 80 90 100 110 120 GNVTTLPITL DQMIYHGKSV VRGVKRAMVV VDMPFGSYQG NEMEGLASAI RIMKESHADA 130 140 150 160 170 180 LKLEGGEEII DTVKRILSAG IPVMGHLGLM PQSINKYGTY TVRAKDDAEA EKLIRDAHLL 190 200 210 220 230 240 EEAGCFGLVL EKIPAALASR VASELTIPVI GIGAGGDVDG QVLVIQDMLG MNNGFRPRFL 250 260 270 RRYADLYTVM TDAISHYVSD VKNCDFPNEK EQY

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006841 Genomic DNA. Translation: BAD48910.1.
RefSeq	YP_099444.1. NC_006347.1.
3D structure databases
ProteinModelPortal	Q64UB9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3084164.
GenomeReviews	Gene locus BF2163 in contig AP006841_GR.
KEGG	bfr:BF2163.
PATRIC	21049978. VBIBacFra17906_2101.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG299908.
OMA	YDATFAH.
PhylomeDB	Q64UB9.
ProtClustDB	PRK00311.
Enzyme and pathway databases
BioCyc	BFRA295405:BF2163-MONOMER.
Family and domain databases
HAMAP	MF_00156. PanB. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
KO	K00606.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR00222. PanB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB_BACFR

Accession

Primary (citable) accession number: Q64UB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program