Reviewed, UniProtKB/Swiss-Prot Q64U80 (FTHS_BACFR)

Names and origin

Protein names

Recommended name:
    Formate--tetrahydrofolate ligase
    EC=6.3.4.3
Alternative name(s):
    Formyltetrahydrofolate synthetase
      Short name=FHS
      Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	BF2202

Organism

Bacteroides fragilis [Complete proteome] [HAMAP]

Taxonomic identifier

817 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_0000199333
Regions
	Nucleotide binding	64 – 71	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64U80-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: E5497181B20F7530	FASTA	555	60,418
	10 20 30 40 50 60 MKSDIEIARS VELKKIKQVA ESIGIPRDEV ENYGRYIAKI PEYLIDEEKV KKSNLILVTA 70 80 90 100 110 120 ITATKAGIGK TTVSIGLALG LNKIGKKAIV ALREPSLGPC FGMKGGAAGG GYAQVLPMEK 130 140 150 160 170 180 INLHFTGDFH AITSAHNMIS ALLDNYLYQN QSKGFGLKEI LWRRVLDVND RSLRNIVVGL 190 200 210 220 230 240 GPKTNGITQE SGFDITPASE IMAILCLSKD VDDLRRRIEN ILLGYTYDNK PFTVKDLGVA 250 260 270 280 290 300 GAITVLLKDA IHPNLVQTTE GTAAFVHGGP FANIAHGCNS ILATKMAMTF GDYVITEAGF 310 320 330 340 350 360 GADLGAEKFY NIKCRKSGLQ PRLTVIVATA QGLKMHGGVS LDRIKEPNLE GLREGLRNLD 370 380 390 400 410 420 KHVRNLHSFG QTVIVAFNKF ASDTDEEMEL LREHCEQLGV GYAINNAFSE GGEGAVDLAN 430 440 450 460 470 480 LVVETIENKP SEPLQFTYND EDSVQQKIEK VATNLYGASV VTYSTLTRNK IKLIEEMGIG 490 500 510 520 530 540 HYPVCIAKTQ YSFSADPKVY GAVDNFELHI KDIVINNGAE MIVAIAGEIM RMPGLPKEPQ 550 ALHIDIVDGN IEGLS

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006841 Genomic DNA. Translation: BAD48949.1.
RefSeq	YP_099483.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3084130.
GenomeReviews	Gene locus BF2202 in contig AP006841_GR.
KEGG	bfr:BF2202.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q64U80.
OMA	GMKGGAT.
Enzyme and pathway databases
BioCyc	BFRA295405:BF2202-MON.
BRENDA	6.3.4.3. 868.
Family and domain databases
HAMAP	MF_01543. [Tree]
InterPro	IPR000559. For_THF_ligase. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_BACFR

Accession

Primary (citable) accession number: Q64U80

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)