Q64TQ1 (SYD_BACFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	BF2379

Organism

Bacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]

Taxonomic identifier

295405 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_0000110824

Sequences

	Sequence		Length	Mass (Da)
	Q64TQ1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 49E0B663CEE58369	FASTA	585	66,169
	10 20 30 40 50 60 MFRTHTCGEL RISDVNKQVK LSGWVQRSRK MGGMTFVDLR DRYGITQLVF NEEIDAELCE 70 80 90 100 110 120 RANKLGREFV IQIVGTVNER FSKNSHIPTG DIEIIVSELN ILNSAITPPF TIEDNTDGGD 130 140 150 160 170 180 DIRMKYRYLD LRRSAVRSNL ELRHKMTIEV RSYLDKLGFL EVETPVLIGS TPEGARDFVV 190 200 210 220 230 240 PSRMNPGQFY ALPQSPQTLK QLLMVSGFDR YFQIAKCFRD EDLRADRQPE FTQIDCEMSF 250 260 270 280 290 300 VEQEDVITTF EGMAKHLFKV IRNIELTGPF PRMPWSEAMR LYGSDKPDIR FGMQFVELMD 310 320 330 340 350 360 ILKGHGFSVF DNATYIGGIC AEGAAGYTRK QLDALTEFVK KPQIGAKGMV YARIEADGTV 370 380 390 400 410 420 KSSVDKFYIQ EVLQQLKEAF GAKPGDLILI LSGDDAMKTR KQLCELRLEM GNQLGLRDKN 430 440 450 460 470 480 TFACLWVVDF PLFEWSEEEG RLMAMHHPFT SPKPEDIHLL DTNPAAVRAN AYDMVINGVE 490 500 510 520 530 540 VGGGSIRIHD SQLQNKMFEL LGFTPERAQE QFGFLMNAFK FGAPPHGGLA YGLDRWVSLF 550 560 570 580 AGLDSIRDCI AFPKNNSGRD VMLDAPAALD PSQLEELNLI VDIKE

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006841 Genomic DNA. Translation: BAD49128.1.
RefSeq	YP_099662.1. NC_006347.1.
3D structure databases
ProteinModelPortal	Q64TQ1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3083937.
GenomeReviews	Gene locus BF2379 in contig AP006841_GR.
KEGG	bfr:BF2379.
NMPDR	fig|295405.3.peg.2223.
PATRIC	21050383. VBIBacFra17906_2298.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	AFPKTQQ.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	BFRA295405:BF2379-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BACFR

Accession

Primary (citable) accession number: Q64TQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program