Q64TJ9 (GCP_BACFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable O-sialoglycoprotein endopeptidase
Short name=Glycoprotease
EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	BF2431

Organism

Bacteroides fragilis [Complete proteome] [HAMAP]

Taxonomic identifier

817 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc Probable.
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_0000303271
Sites
	Metal binding	111	1	Zinc Potential
	Metal binding	115	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q64TJ9-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: F7521CDA5B140350	FASTA	339	36,956
	10 20 30 40 50 60 MSTIILGIES SCDDTSAAVI KDGYLLSNVV SSQAVHEAYG GVVPELASRA HQQNIVPVVH 70 80 90 100 110 120 EALKRAGVTK EELSAVAFTR GPGLMGSLLV GVSFAKGFAR SLNIPMIDVN HLTGHVLAHF 130 140 150 160 170 180 IKEEGEANEQ PDFPFLCLLV SGGNSQIILV KAYNDMEILG QTIDDAAGEA IDKCSKVMGL 190 200 210 220 230 240 GYPGGPIIDR LARQGNPKAY TFSKPHISGL DYSFSGLKTS FLYSLRDWMK EDPDFIEHHK 250 260 270 280 290 300 NDLAASLEAT VVDILMDKLR KAAKQYKINE VAVAGGVSAN NGLRNAFREH AEKYGWKIFI 310 320 330 PKFSYTTDNA AMIAITGYFK YQDKDFCSIE QPAYSRVTL

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006841 Genomic DNA. Translation: BAD49180.1.
RefSeq	YP_099714.1.
3D structure databases
ProteinModelPortal	Q64TJ9.
SMR	Q64TJ9. Positions 4-331.
ModBase	Search...
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	3083908.
GenomeReviews	Gene locus BF2431 in contig AP006841_GR.
KEGG	bfr:BF2431.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304663.
OMA	MEVIGQT.
PhylomeDB	Q64TJ9.
ProtClustDB	PRK09604.
Enzyme and pathway databases
BioCyc	BFRA295405:BF2431-MONOMER.
BRENDA	3.4.24.57. 868.
Family and domain databases
HAMAP	MF_01445. Glycoptase_bact. [Tree]
InterPro	IPR022450. Pept_M22_O-sialoglycopept. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR03723. Bact_gcp. 1 hit. TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_BACFR

Accession

Primary (citable) accession number: Q64TJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 25, 2004
Last modified:	August 10, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)