Q64TJ6 (SYP_BACFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	BF2434

Organism

Bacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]

Taxonomic identifier

295405 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	Proline--tRNA ligase HAMAP MF_01571		PRO_0000249121

Sequences

	Sequence		Length	Mass (Da)
	Q64TJ6 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: DEAD2CB76F1284E0	FASTA	497	56,954
	10 20 30 40 50 60 MAKELKDLTK RSENYSQWYN DLVVKADLAE QSAVRGCMVI KPYGYAIWEK MQRQLDDMFK 70 80 90 100 110 120 ETGHVNAYFP LLIPKSFLSR EAEHVEGFAK ECAVVTHYRL KNAEDGSGVV VDPAAKLEEE 130 140 150 160 170 180 LIIRPTSETI IWNTYKNWIQ SYRDLPILCN QWANVFRWEM RTRLFLRTAE FLWQEGHTAH 190 200 210 220 230 240 ATREEAEEEA IRMLNVYAEF AEKYMAVPVV KGVKSANERF AGALDTYTIE AMMQDGKALQ 250 260 270 280 290 300 SGTSHFLGQN FAKAFDVQFV NKENKLEYVW ATSWGVSTRL MGALIMTHSD DNGLVLPPHL 310 320 330 340 350 360 APIQVVIVPI YKNDEQLKLI DAKVEGIVAR LKQLGISVKY DNADNKRPGF KFADYELKGV 370 380 390 400 410 420 PVRLVMGGRD LENNTMEVMR RDTLEKETVT CDGIETYVQN LLEEIQANIY KKARTYRDSR 430 440 450 460 470 480 ITTVDSYDEF KEKIEEGGFI LAHWDGTVET EEKIKEETKA TIRCIPFESF VEGDKEPGKC 490 MVTGKPSACR VIFARSY

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006841 Genomic DNA. Translation: BAD49183.1.
RefSeq	YP_099717.1. NC_006347.1.
3D structure databases
HSSP	HSSP built from PDB template 1NJ1 based on UniProtKB O26708.
ProteinModelPortal	Q64TJ6.
SMR	Q64TJ6. Positions 6-497.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3083839.
GenomeReviews	Gene locus BF2434 in contig AP006841_GR.
KEGG	bfr:BF2434.
PATRIC	21050497. VBIBacFra17906_2352.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG334108.
OMA	KFAEYEL.
PhylomeDB	Q64TJ6.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	BFRA295405:BF2434-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_BACFR

Accession

Primary (citable) accession number: Q64TJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program