Q64TE4 (COBT_BACFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Short name=NN:DBI PRT
EC=2.4.2.21

Alternative name(s):
N(1)-alpha-phosphoribosyltransferase

Gene names

Name:	cobT
Ordered Locus Names:	BF2486

Organism

Bacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]

Taxonomic identifier

295405 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB). HAMAP MF_00230
Catalytic activity	Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate. HAMAP MF_00230
Pathway	Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-ribazole from 5,6-dimethylbenzimidazole: step 1/2. HAMAP MF_00230
Sequence similarities	Belongs to the CobT family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase HAMAP MF_00230		PRO_1000021576
Sites
	Active site	312	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64TE4 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: FA08158B1A46EE23	FASTA	345	37,869
	10 20 30 40 50 60 MKTFQITRPD ETIREALTDK INNLTKPKGS LGTLEELALQ IGLIQQTLTP ELRHPQNIIF 70 80 90 100 110 120 AADHGIVDEG VSLSPKEITW QQISNFLHGG AGVNFLCRQH GFELKIVDAG VDYDLPYEKG 130 140 150 160 170 180 IINMKVRKSS RNYLYEAAMT EEEMNLCIER GAEVVRQCHA EGCNVLSLGE MGIGNTSSSS 190 200 210 220 230 240 MWMTCFTHIP LELCVGAGSG LDNAGVHHKY NVLQQALDHY QGDGSAHDLI RYFGGLEMVM 250 260 270 280 290 300 AIGAMLQAAE LKMIILVDGF IMTNCILAAS QLYPEVLHYA IFGHQGDEAG HKLVLDAMGA 310 320 330 340 KPLLNLGLRL GEGTGAICSY PIIDSAIRMI NEMDNFAHAA ITKYF

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006841 Genomic DNA. Translation: BAD49235.1.
RefSeq	YP_099769.1. NC_006347.1.
3D structure databases
HSSP	HSSP built from PDB template 1WX1 based on UniProtKB Q7SIC7.
ProteinModelPortal	Q64TE4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3083818.
GenomeReviews	Gene locus BF2486 in contig AP006841_GR.
KEGG	bfr:BF2486.
NMPDR	fig|295405.3.peg.2852.
PATRIC	21050583. VBIBacFra17906_2394.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG634399.
OMA	FICSVAA.
ProtClustDB	CLSK826801.
Enzyme and pathway databases
BioCyc	BFRA295405:BF2486-MONOMER.
Family and domain databases
HAMAP	MF_00230. CobT. [Tree]
InterPro	IPR003200. Nict_dMeBzImd_PRibTrfase-like. IPR023195. Nict_dMeBzImd_PRibTrfase_N. IPR017846. Nict_dMeBzImd_PRibTrfase_pro. [Graphical view]
Gene3D	G3DSA:1.10.1610.10. Nict_dMeBzImd_PRibTrfase_N. 1 hit.
KO	K00768.
Pfam	PF02277. DBI_PRT. 1 hit. [Graphical view]
SUPFAM	SSF52733. NN:DBI_PRT. 1 hit.
TIGRFAMs	TIGR03160. CobT_DBIPRT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COBT_BACFR

Accession

Primary (citable) accession number: Q64TE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program