Q64RZ8 (MURB_BACFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BF2985

Organism

Bacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]

Taxonomic identifier

295405 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000224662
Regions
	Domain	15 – 184	170	FAD-binding PCMH-type
Sites
	Active site	160	1	By similarity
	Active site	232	1	Proton donor By similarity
	Active site	328	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64RZ8 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 1CAEDEFBE9458A37	FASTA	332	36,930
	10 20 30 40 50 60 MEQKYSLLSH NTFGIDVSAA CFLEYASVDE LRGLIGSGRV TSPYLHIGGG SNLLFTKDYE 70 80 90 100 110 120 GTILHSRIGG VEVVAETDDD IVVRVGAGVV WDDFVDYCVQ RHWYGVENLS LIPGEVGASA 130 140 150 160 170 180 VQNIGAYGVE VKDLIVRVET LNIEGKEHVY DVTECGYSYR DSIFKRPENK SVFVTYVSFR 190 200 210 220 230 240 LSKREHYTLD YGTIRRELEK YPGVTLDVVR RVIIAIREEK LPDPRVMGNA GSFFMNPIVG 250 260 270 280 290 300 REQFEALQAE YPQMPFYEID TDRVKIPAGW MIDQCGWKGK ALGPAAVHDK QALVLVNRGG 310 320 330 AKGADVIALS DAVRASVRAK FGIDIHPEVN FI

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006841 Genomic DNA. Translation: BAD49733.1.
RefSeq	YP_100267.1. NC_006347.1.
3D structure databases
ProteinModelPortal	Q64RZ8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3083336.
GenomeReviews	Gene locus BF2985 in contig AP006841_GR.
KEGG	bfr:BF2985.
PATRIC	21051548. VBIBacFra17906_2865.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG686573.
OMA	IEQAGWK.
ProtClustDB	PRK00046.
Enzyme and pathway databases
BioCyc	BFRA295405:BF2985-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BACFR

Accession

Primary (citable) accession number: Q64RZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program