Q64RQ5 (RLMF_BACFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	BF3081

Organism

Bacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]

Taxonomic identifier

295405 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349895

Sequences

	Sequence		Length	Mass (Da)
	Q64RQ5 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 187BA0521815A372	FASTA	305	34,823
	10 20 30 40 50 60 MAERNELHKR NRHNGQYDFS RLTEEYPPLK KFIVLNAYGT TSIDFFNPRA VKALNKALLI 70 80 90 100 110 120 SCYGIRYWDI PKNYLCPPIP GRADYIHYIA DLIQPDISDE STGLKTAIPN ARQYRCLDIG 130 140 150 160 170 180 VGANCIYPII GQTEYGWTFV GSDIDPVSID NARKIVTCNP ALAHKIELRL QRDSRKIFEG 190 200 210 220 230 240 IIAPNEYFDV TLCNPPFHSS KEEAEDGTLR KLSSLKGKKV TKARLNFGGN ANELWCEGGE 250 260 270 280 290 300 LRFLLTMIEE SRNYRKNCGW FTSLVSKEKN LGKLTAKLKS TDIAEHRIIE MHQGTKTSRI LAWRF

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006841 Genomic DNA. Translation: BAD49826.1.
RefSeq	YP_100360.1. NC_006347.1.
3D structure databases
ProteinModelPortal	Q64RQ5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3083204.
GenomeReviews	Gene locus BF3081 in contig AP006841_GR.
KEGG	bfr:BF3081.
NMPDR	fig|295405.3.peg.3438.
PATRIC	21051716. VBIBacFra17906_2947.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG747880.
OMA	DFANPEA.
ProtClustDB	PRK11727.
Enzyme and pathway databases
BioCyc	BFRA295405:BF3081-MONOMER.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_BACFR

Accession

Primary (citable) accession number: Q64RQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program