Q64PS6 (ILVD_BACFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BF3763

Organism

Bacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]

Taxonomic identifier

295405 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000225371
Sites
	Metal binding	123	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	192	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q64PS6 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: CE1C78D83FBD9302	FASTA	600	64,371
	10 20 30 40 50 60 MKKQLRSSFS TQGRRMAGAR ALWAANGMKK EQLGKPIIAI VNSFTQFVPG HVHLHEIGQL 70 80 90 100 110 120 VKKEIEKLGC FAAEFNTIAI DDGIAMGHDG MLYSLPSRDI IADSVEYMVN AHKADAMVCI 130 140 150 160 170 180 SNCDKITPGM LMAAMRLNIP TVFVSGGPME AGELDGQHLD LIDAMIKSAD ESVSDEEVSK 190 200 210 220 230 240 IENRACPTCG CCSGMFTANS MNCLNEAIGL ALPGNGTIVA THANRTQLFK DAAKQIVENT 250 260 270 280 290 300 YKYYRDGDES VLPRSIATRE AFLNAMTLDI AMGGSTNTVL HLLAIAHEAE ADFKMDDIDM 310 320 330 340 350 360 LSRKSPCLCK VAPNTQKYHI QDVNRAGGIM GIMGQLAKAG LIDTSVVRID GMTLGEAIDK 370 380 390 400 410 420 YDITSPNVCE EAIKKYKSAA AGKFNLVLGS QDVYYKELDT DRAEGCIRDI EHAYSKDGGL 430 440 450 460 470 480 AVLKGNIAQD GCVVKTAGVD ESIWKFTGPA KVFDSQEAAC NGILGGKVVS GDVVVITHEG 490 500 510 520 530 540 PKGGPGMQEM LYPTSYIKSR HLGKECALIT DGRFSGGTSG LSIGHISPEA AAGGNIGKIK 550 560 570 580 590 600 DGDIIEINIP ERTINVRLTD EELAARPMTP VTRERYVTKS LKAYASMVSS ADKGAVRLID

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006841 Genomic DNA. Translation: BAD50505.1.
RefSeq	YP_101039.1. NC_006347.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3084844.
GenomeReviews	Gene locus BF3763 in contig AP006841_GR.
KEGG	bfr:BF3763.
NMPDR	fig|295405.3.peg.3816.
PATRIC	21053066. VBIBacFra17906_3611.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	BFRA295405:BF3763-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BACFR

Accession

Primary (citable) accession number: Q64PS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program