Q64PM4 (ASNA_BACFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	BF3815

Organism

Bacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]

Taxonomic identifier

295405 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017937

Sequences

	Sequence		Length	Mass (Da)
	Q64PM4 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 1951B8063797CA3E	FASTA	345	39,996
	10 20 30 40 50 60 MSYLIKPQNY KPLLDLKQTE LGIKQIKEFF QLNLSSELRL RRVTAPLFVL KGMGINDDLN 70 80 90 100 110 120 GIERPVSFPI KDLGDAQAEV VHSLAKWKRL TLADYHIEPG YGIYTDMNAI RSDEELGNLH 130 140 150 160 170 180 SLYVDQWDWE RVITAEDRNA DFLKEIVNRI YAAMIRTEYM VYEMYPQIKP CLPQKLHFIH 190 200 210 220 230 240 SEELRQLYPD MEPKCREHAI CKKYGAVFII GIGCKLSDGK KHDGRAPDYD DYTSKGLNDL 250 260 270 280 290 300 PGLNGDLLLW DDVLQRSIEL SSMGIRVDKE ALLRQVKQEN QEQRLELYFH KRLLNDTLPL 310 320 330 340 SIGGGIGQSR LCMFYLRKAH IGEIQASIWP EEMRRECTAL NIHLI

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006841 Genomic DNA. Translation: BAD50557.1.
RefSeq	YP_101091.1. NC_006347.1.
3D structure databases
HSSP	HSSP built from PDB template 12AS based on UniProtKB P00963.
ProteinModelPortal	Q64PM4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3084896.
GenomeReviews	Gene locus BF3815 in contig AP006841_GR.
KEGG	bfr:BF3815.
PATRIC	21053172. VBIBacFra17906_3663.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
PhylomeDB	Q64PM4.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	BFRA295405:BF3815-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_BACFR

Accession

Primary (citable) accession number: Q64PM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program