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Q64P45 (TRMB_BACFR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:BF3995
OrganismBacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]
Taxonomic identifier295405 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

Protein attributes

Sequence length252 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

tRNA methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 252252tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288122

Regions

Region199 – 2024Substrate binding By similarity

Sites

Active site1251 By similarity
Binding site511S-adenosyl-L-methionine By similarity
Binding site761S-adenosyl-L-methionine By similarity
Binding site1031S-adenosyl-L-methionine By similarity
Binding site1251S-adenosyl-L-methionine By similarity
Binding site1291Substrate By similarity
Binding site1591Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q64P45 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 99FB41C5CD8A87ED

FASTA25229,479
        10         20         30         40         50         60 
MGKNKLEKFA DMASYPHVFE YPYSAVDNVP FDMKGKWHKE FFKNDNPIVL ELGCGRGEYT 

        70         80         90        100        110        120 
VGLGRMFPDK NFIAVDIKGA RMWTGATESL QAGMKNVAFL RTNIEIIDRF FAEGEVSEIW 

       130        140        150        160        170        180 
LTFSDPQMKK ATKRLTSTYF MERYRKFLVS NGIIHLKTDS NFMFTYTKYM IEENGLPVEF 

       190        200        210        220        230        240 
ITEDLYHSDL VDDILGIKTY YEQQWLDRGL SIKYIKFLLP QEGELREPDI EIELDSYRSY 

       250 
NRSKRSGLQT SK 

« Hide

References

[1]"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation."
Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y.
Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: YCH46.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006841 Genomic DNA. Translation: BAD50737.1.
RefSeqYP_101271.1. NC_006347.1.

3D structure databases

ProteinModelPortalQ64P45.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING295405.BF3995.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD50737; BAD50737; BF3995.
GeneID3085076.
KEGGbfr:BF3995.
PATRIC21053544. VBIBacFra17906_3844.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMAKTDDDEL.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_BACFR
AccessionPrimary (citable) accession number: Q64P45
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 25, 2004
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways