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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Bacteroides fragilis (strain YCH46)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei51S-adenosyl-L-methionineUniRule annotation1
Binding sitei76S-adenosyl-L-methionineUniRule annotation1
Binding sitei103S-adenosyl-L-methionineUniRule annotation1
Active sitei125By similarity1
Binding sitei125S-adenosyl-L-methionineUniRule annotation1
Binding sitei129SubstrateUniRule annotation1
Binding sitei159SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:BF3995
OrganismiBacteroides fragilis (strain YCH46)
Taxonomic identifieri295405 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000002197 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002881221 – 252tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST252

Structurei

3D structure databases

ProteinModelPortaliQ64P45.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni199 – 202Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

KOiK03439.
OMAiFPDPQIK.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64P45-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKNKLEKFA DMASYPHVFE YPYSAVDNVP FDMKGKWHKE FFKNDNPIVL
60 70 80 90 100
ELGCGRGEYT VGLGRMFPDK NFIAVDIKGA RMWTGATESL QAGMKNVAFL
110 120 130 140 150
RTNIEIIDRF FAEGEVSEIW LTFSDPQMKK ATKRLTSTYF MERYRKFLVS
160 170 180 190 200
NGIIHLKTDS NFMFTYTKYM IEENGLPVEF ITEDLYHSDL VDDILGIKTY
210 220 230 240 250
YEQQWLDRGL SIKYIKFLLP QEGELREPDI EIELDSYRSY NRSKRSGLQT

SK
Length:252
Mass (Da):29,479
Last modified:October 25, 2004 - v1
Checksum:i99FB41C5CD8A87ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006841 Genomic DNA. Translation: BAD50737.1.
RefSeqiWP_005791725.1. NC_006347.1.
YP_101271.1. NC_006347.1.

Genome annotation databases

EnsemblBacteriaiBAD50737; BAD50737; BF3995.
GeneIDi3085076.
KEGGibfr:BF3995.
PATRICi21053544. VBIBacFra17906_3844.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006841 Genomic DNA. Translation: BAD50737.1.
RefSeqiWP_005791725.1. NC_006347.1.
YP_101271.1. NC_006347.1.

3D structure databases

ProteinModelPortaliQ64P45.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD50737; BAD50737; BF3995.
GeneIDi3085076.
KEGGibfr:BF3995.
PATRICi21053544. VBIBacFra17906_3844.

Phylogenomic databases

KOiK03439.
OMAiFPDPQIK.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_BACFR
AccessioniPrimary (citable) accession number: Q64P45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 25, 2004
Last modified: September 7, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.