Reviewed, UniProtKB/Swiss-Prot Q64P24 (SYN_BACFR)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	BF4016

Organism

Bacteroides fragilis [Complete proteome] [HAMAP]

Taxonomic identifier

817 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00534
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_0000176389

Sequences

	Sequence		Length	Mass (Da)
	Q64P24-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 91F4162F29FB76EC	FASTA	467	53,518
	10 20 30 40 50 60 MEKISRTKIV DLMKREDFGA MVNVKGWVRT RRGSKQVNFI ALNDGSTINN VQVVVDLANF 70 80 90 100 110 120 DEEMLKQITT GACLSVNGVL TESVGAGQKA EVQAREIEVL GTCDNTYPLQ KKGHSMEFLR 130 140 150 160 170 180 EIAHLRPRTN TFGAVFRIRH NMAIAIHKFF HEKGFFYFHT PIITASDCEG AGQMFQVTTM 190 200 210 220 230 240 NLYDLKKDEN GSIVYDDDFF GKQASLTVSG QLEGELAATA LGAIYTFGPT FRAENSNTPR 250 260 270 280 290 300 HLAEFWMIEP EVAFNEIQEN MDLAEEFIKY CVRWALDNCA DDVKFLNDMF DKGLIERLEG 310 320 330 340 350 360 VLKEDFVRLP YTEGIKILEE AVAKGHKFEF PVYWGVDLAS EHERYLVEDH FKRPVILTDY 370 380 390 400 410 420 PKEIKAFYMK QNEDGKTVRA MDVLFPKIGE IIGGSERESD YNKLMMRIEE MHIPMKDMWW 430 440 450 460 YLDTRKFGTC PHSGFGLGFE RLLLFVTGMS NIRDVIPFPR TPRNADF

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006841 Genomic DNA. Translation: BAD50758.1.
RefSeq	YP_101292.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3085095.
GenomeReviews	Gene locus BF4016 in contig AP006841_GR.
KEGG	bfr:BF4016.
NMPDR	fig|295405.3.peg.4239.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q64P24.
OMA	LQKKRHS.
Enzyme and pathway databases
BioCyc	BFRA295405:BF4016-MON.
BRENDA	6.1.1.22. 868.
Family and domain databases
HAMAP	MF_00534. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_BACFR

Accession

Primary (citable) accession number: Q64P24

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)