Reviewed, UniProtKB/Swiss-Prot Q64NP4 (HUTI_BACFR)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	BF4146

Organism

Bacteroides fragilis [Complete proteome] [HAMAP]

Taxonomic identifier

817 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Imidazolonepropionase HAMAP MF_00372		PRO_0000306435
Sites
	Metal binding	80	1	Zinc or iron By similarity
	Metal binding	82	1	Zinc or iron By similarity
	Metal binding	252	1	Zinc or iron By similarity
	Metal binding	326	1	Zinc or iron By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	102	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity
	Binding site	187	1	Substrate By similarity
	Binding site	255	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q64NP4-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 5FF02EC98FEA449A	FASTA	417	45,478
	10 20 30 40 50 60 MSENLIIFNA KVVTPLGFSA RKGKEMAELR ILEKATVEVV DGIITYVGPN RGEVRDGYYH 70 80 90 100 110 120 HFWHYNARGK CLLPGFVDSH THFVFGGERA EEFSWRLKGE SYMSIMQRGG GIASTVQATR 130 140 150 160 170 180 ELSFIHLRSK AEGLLKKMTA MGITTVEGKS GYGLNRETEL LQLKVMRSLN KDEGVRVDIV 190 200 210 220 230 240 PTFLGAHALP DEYRERPDDY IDFLIRELLP VIQRDSLAEF CDVFCEEGVF SIEQSRRLLT 250 260 270 280 290 300 AAGDYGLLPK LHADEIVPLG GAELAAELGA VSADHLLHAS DAGIEAMARK GVVATLLPLT 310 320 330 340 350 360 AFALKEPYAR GRDMIDAGCA VALATDLNPG SCFSGSIPLT FALACIHMQL TVEEAITALT 370 380 390 400 410 LNGAAALNRA DSIGSIEVGK KGDFVVLDSD NYHILPYYVG MNCVNTTIKG GMLYPSV

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006841 Genomic DNA. Translation: BAD50888.1.
RefSeq	YP_101422.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3085227.
GenomeReviews	Gene locus BF4146 in contig AP006841_GR.
KEGG	bfr:BF4146.
NMPDR	fig|295405.3.peg.4111.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q64NP4.
OMA	MNMACTL.
Enzyme and pathway databases
BioCyc	BFRA295405:BF4146-MON.
BRENDA	3.5.2.7. 868.
Family and domain databases
HAMAP	MF_00372. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. [Graphical view]
Pfam	PF01979. Amidohydro_1. 2 hits. [Graphical view]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_BACFR

Accession

Primary (citable) accession number: Q64NP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)