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Q64MG4 (SYL_BACFR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BF4586
OrganismBacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]
Taxonomic identifier295405 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

Protein attributes

Sequence length943 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 943943Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151968

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif717 – 7215"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7201ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q64MG4 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 9F9A3355FAA30082

FASTA943107,969
        10         20         30         40         50         60 
MEYNFREIEK KWQKIWVDNH TYQVNEDASK QKFYVLNMFP YPSGAGLHVG HPLGYIASDI 

        70         80         90        100        110        120 
YARYKRLQGF NVLNPMGYDA YGLPAEQYAI QTGQHPAITT VNNINRYREQ LDKIGFSFDW 

       130        140        150        160        170        180 
NREIRTCDPE YYHWTQWAFI KMFNSYYCND EKQARPIEEL IEAFSTNGTQ GMNVACGEEM 

       190        200        210        220        230        240 
DFTADEWNAK SEKEQQEILM NYRIAYLGNT MVNWCPALGT VLANDEVVDG VSERGGYPVI 

       250        260        270        280        290        300 
QKVMRQWCLR VSAYAQRLLD GLETVEWTDS LKETQRNWIG RSEGAEMNFK VKDSDIEFTI 

       310        320        330        340        350        360 
FTTRADTVFG VTFMVLAPES ELVAKLTTPE QKAEVDAYLD RTKKRTERER IADRSVSGVF 

       370        380        390        400        410        420 
SGSYAINPLT NEPIPVWISD YVLAGYGTGA IMAVPAHDSR DYAFAKHFNL EIRPLIEGCD 

       430        440        450        460        470        480 
VSEESFDAKE GIMMNSPRPG APEGGLVLNG LTVKEAIAKT KEYIKATGLG RVKVNFRLRD 

       490        500        510        520        530        540 
AIFSRQRYWG EPFPVYYKDG MPYMIDESCL PLELPEVAKF LPTETGEPPL GHATKWAWDT 

       550        560        570        580        590        600 
VNKCVTDNEN IDNRTIFPLE LNTMPGFAGS SAYYLRYMDP RNHEALVSPA VDQYWKNVDL 

       610        620        630        640        650        660 
YVGGTEHATG HLIYSRFWNK FLHDWGISVA EEPFQKLVNQ GMIQGRSNFV YRIKDTNTFV 

       670        680        690        700        710        720 
SLNLKDQYEV TPIHVDVNIV SNDILDLEAF KAWRPEYETA EFILEDGKYI CGWAVEKMSK 

       730        740        750        760        770        780 
SMFNVVNPDM IVEKYGADTL RMYEMFLGPV EQSKPWDTNG IDGVHRFIKK FWSLFYDRNG 

       790        800        810        820        830        840 
EYLVKDEPAT KEELKALHKL IKKVTGDIEQ FSYNTSVSAF MICVNELSSL KCNKKEVLEQ 

       850        860        870        880        890        900 
LIVVLAPFAP HVCEELWDTL GNTTSVCDAQ WPTFNEQYLV EDTVNYTISF NGKARFNMEF 

       910        920        930        940 
PADAASDAIQ ATVLADERSL KWTEGKTPKK VIVVPKKIVN IVI 

« Hide

References

[1]"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation."
Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y.
Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: YCH46.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006841 Genomic DNA. Translation: BAD51323.1.
RefSeqYP_101857.1. NC_006347.1.

3D structure databases

ProteinModelPortalQ64MG4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING295405.BF4586.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD51323; BAD51323; BF4586.
GeneID3085662.
KEGGbfr:BF4586.
PATRIC21054733. VBIBacFra17906_4410.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAMAFAGPP.
OrthoDBEOG63Z74X.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BACFR
AccessionPrimary (citable) accession number: Q64MG4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 25, 2004
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries