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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Bacteroides fragilis (strain YCH46)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei720 – 7201ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:BF4586
OrganismiBacteroides fragilis (strain YCH46)
Taxonomic identifieri295405 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
ProteomesiUP000002197 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Leucine--tRNA ligasePRO_0000151968Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ64MG4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi717 – 7215"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q64MG4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYNFREIEK KWQKIWVDNH TYQVNEDASK QKFYVLNMFP YPSGAGLHVG
60 70 80 90 100
HPLGYIASDI YARYKRLQGF NVLNPMGYDA YGLPAEQYAI QTGQHPAITT
110 120 130 140 150
VNNINRYREQ LDKIGFSFDW NREIRTCDPE YYHWTQWAFI KMFNSYYCND
160 170 180 190 200
EKQARPIEEL IEAFSTNGTQ GMNVACGEEM DFTADEWNAK SEKEQQEILM
210 220 230 240 250
NYRIAYLGNT MVNWCPALGT VLANDEVVDG VSERGGYPVI QKVMRQWCLR
260 270 280 290 300
VSAYAQRLLD GLETVEWTDS LKETQRNWIG RSEGAEMNFK VKDSDIEFTI
310 320 330 340 350
FTTRADTVFG VTFMVLAPES ELVAKLTTPE QKAEVDAYLD RTKKRTERER
360 370 380 390 400
IADRSVSGVF SGSYAINPLT NEPIPVWISD YVLAGYGTGA IMAVPAHDSR
410 420 430 440 450
DYAFAKHFNL EIRPLIEGCD VSEESFDAKE GIMMNSPRPG APEGGLVLNG
460 470 480 490 500
LTVKEAIAKT KEYIKATGLG RVKVNFRLRD AIFSRQRYWG EPFPVYYKDG
510 520 530 540 550
MPYMIDESCL PLELPEVAKF LPTETGEPPL GHATKWAWDT VNKCVTDNEN
560 570 580 590 600
IDNRTIFPLE LNTMPGFAGS SAYYLRYMDP RNHEALVSPA VDQYWKNVDL
610 620 630 640 650
YVGGTEHATG HLIYSRFWNK FLHDWGISVA EEPFQKLVNQ GMIQGRSNFV
660 670 680 690 700
YRIKDTNTFV SLNLKDQYEV TPIHVDVNIV SNDILDLEAF KAWRPEYETA
710 720 730 740 750
EFILEDGKYI CGWAVEKMSK SMFNVVNPDM IVEKYGADTL RMYEMFLGPV
760 770 780 790 800
EQSKPWDTNG IDGVHRFIKK FWSLFYDRNG EYLVKDEPAT KEELKALHKL
810 820 830 840 850
IKKVTGDIEQ FSYNTSVSAF MICVNELSSL KCNKKEVLEQ LIVVLAPFAP
860 870 880 890 900
HVCEELWDTL GNTTSVCDAQ WPTFNEQYLV EDTVNYTISF NGKARFNMEF
910 920 930 940
PADAASDAIQ ATVLADERSL KWTEGKTPKK VIVVPKKIVN IVI
Length:943
Mass (Da):107,969
Last modified:October 25, 2004 - v1
Checksum:i9F9A3355FAA30082
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006841 Genomic DNA. Translation: BAD51323.1.
RefSeqiWP_011203753.1. NC_006347.1.
YP_101857.1. NC_006347.1.

Genome annotation databases

EnsemblBacteriaiBAD51323; BAD51323; BF4586.
GeneIDi3085662.
KEGGibfr:BF4586.
PATRICi21054733. VBIBacFra17906_4410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006841 Genomic DNA. Translation: BAD51323.1.
RefSeqiWP_011203753.1. NC_006347.1.
YP_101857.1. NC_006347.1.

3D structure databases

ProteinModelPortaliQ64MG4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD51323; BAD51323; BF4586.
GeneIDi3085662.
KEGGibfr:BF4586.
PATRICi21054733. VBIBacFra17906_4410.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation."
    Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y.
    Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YCH46.

Entry informationi

Entry nameiSYL_BACFR
AccessioniPrimary (citable) accession number: Q64MG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 25, 2004
Last modified: July 22, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.