ID   WDR25_HUMAN             Reviewed;         544 AA.
AC   Q64LD2; A8K7E5; Q6NVV6; Q86TQ4; Q9BTK5;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   20-FEB-2007, sequence version 3.
DT   27-MAR-2024, entry version 158.
DE   RecName: Full=WD repeat-containing protein 25;
GN   Name=WDR25 {ECO:0000312|HGNC:HGNC:21064};
GN   Synonyms=C14orf67 {ECO:0000312|HGNC:HGNC:21064};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND VARIANT
RP   ARG-88.
RC   TISSUE=Fetal brain;
RX   PubMed=15587985; DOI=10.1023/b:bigi.0000043954.64202.61;
RA   Jin F., Dai J., Ji C., Gu S., Wu M., Qian J., Xie Y., Mao Y.;
RT   "A novel human gene (WDR25) encoding a 7-WD40-containing protein maps on
RT   14q32.";
RL   Biochem. Genet. 42:419-427(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANTS ARG-88 AND
RP   ARG-149.
RC   TISSUE=Neuroblastoma;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12508121; DOI=10.1038/nature01348;
RA   Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C.,
RA   Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A.,
RA   Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H.,
RA   Du H., Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T.,
RA   Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B.,
RA   Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D.,
RA   Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R.,
RA   Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S.,
RA   Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C.,
RA   Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S.,
RA   Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C.,
RA   Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P.,
RA   Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M.,
RA   Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V.,
RA   Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J.,
RA   Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F.,
RA   Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F.,
RA   Waterston R., Hood L., Weissenbach J.;
RT   "The DNA sequence and analysis of human chromosome 14.";
RL   Nature 421:601-607(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Muscle;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-275 (ISOFORM 1).
RC   TISSUE=Neuroblastoma;
RA   Li W.B., Gruber C., Jessee J., Polayes D.;
RT   "Full-length cDNA libraries and normalization.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- INTERACTION:
CC       Q64LD2; Q15323: KRT31; NbExp=3; IntAct=EBI-744560, EBI-948001;
CC       Q64LD2; Q6A162: KRT40; NbExp=3; IntAct=EBI-744560, EBI-10171697;
CC       Q64LD2; Q7Z3S9: NOTCH2NLA; NbExp=3; IntAct=EBI-744560, EBI-945833;
CC       Q64LD2; Q8ND90: PNMA1; NbExp=3; IntAct=EBI-744560, EBI-302345;
CC       Q64LD2; P36406: TRIM23; NbExp=3; IntAct=EBI-744560, EBI-740098;
CC       Q64LD2-2; Q5BKX5-3: ACTMAP; NbExp=3; IntAct=EBI-12032042, EBI-11976299;
CC       Q64LD2-2; Q9ULX6: AKAP8L; NbExp=3; IntAct=EBI-12032042, EBI-357530;
CC       Q64LD2-2; Q9BYD5: CNFN; NbExp=3; IntAct=EBI-12032042, EBI-12819063;
CC       Q64LD2-2; A8MQ03: CYSRT1; NbExp=4; IntAct=EBI-12032042, EBI-3867333;
CC       Q64LD2-2; Q92997: DVL3; NbExp=3; IntAct=EBI-12032042, EBI-739789;
CC       Q64LD2-2; A1KXE4-2: FAM168B; NbExp=3; IntAct=EBI-12032042, EBI-12193763;
CC       Q64LD2-2; Q13643: FHL3; NbExp=3; IntAct=EBI-12032042, EBI-741101;
CC       Q64LD2-2; Q12951-2: FOXI1; NbExp=3; IntAct=EBI-12032042, EBI-12018822;
CC       Q64LD2-2; A6NEM1: GOLGA6L9; NbExp=3; IntAct=EBI-12032042, EBI-5916454;
CC       Q64LD2-2; O75031: HSF2BP; NbExp=3; IntAct=EBI-12032042, EBI-7116203;
CC       Q64LD2-2; Q15323: KRT31; NbExp=3; IntAct=EBI-12032042, EBI-948001;
CC       Q64LD2-2; O76011: KRT34; NbExp=3; IntAct=EBI-12032042, EBI-1047093;
CC       Q64LD2-2; P60328: KRTAP12-3; NbExp=3; IntAct=EBI-12032042, EBI-11953334;
CC       Q64LD2-2; Q3LI76: KRTAP15-1; NbExp=3; IntAct=EBI-12032042, EBI-11992140;
CC       Q64LD2-2; Q9BYR8: KRTAP3-1; NbExp=3; IntAct=EBI-12032042, EBI-9996449;
CC       Q64LD2-2; O95751: LDOC1; NbExp=3; IntAct=EBI-12032042, EBI-740738;
CC       Q64LD2-2; Q8TBB1: LNX1; NbExp=3; IntAct=EBI-12032042, EBI-739832;
CC       Q64LD2-2; Q13064: MKRN3; NbExp=3; IntAct=EBI-12032042, EBI-2340269;
CC       Q64LD2-2; P0DPK4: NOTCH2NLC; NbExp=3; IntAct=EBI-12032042, EBI-22310682;
CC       Q64LD2-2; Q99471: PFDN5; NbExp=3; IntAct=EBI-12032042, EBI-357275;
CC       Q64LD2-2; Q9GZV8: PRDM14; NbExp=3; IntAct=EBI-12032042, EBI-3957793;
CC       Q64LD2-2; P61289: PSME3; NbExp=3; IntAct=EBI-12032042, EBI-355546;
CC       Q64LD2-2; Q96N21: TEPSIN; NbExp=3; IntAct=EBI-12032042, EBI-11139477;
CC       Q64LD2-2; Q5T124-6: UBXN11; NbExp=3; IntAct=EBI-12032042, EBI-11524408;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q64LD2-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q64LD2-2; Sequence=VSP_021331, VSP_021332;
CC   -!- TISSUE SPECIFICITY: Expressed in heart, muscle, testis, ovary, uterus
CC       and prostate. {ECO:0000269|PubMed:15587985}.
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DR   EMBL; AY295082; AAQ63174.1; -; mRNA.
DR   EMBL; AK291960; BAF84649.1; -; mRNA.
DR   EMBL; AL135838; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AL845552; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC003641; AAH03641.1; -; mRNA.
DR   EMBL; BX248767; CAD66574.1; -; mRNA.
DR   CCDS; CCDS32157.1; -. [Q64LD2-1]
DR   RefSeq; NP_001154948.1; NM_001161476.1. [Q64LD2-1]
DR   RefSeq; NP_078791.3; NM_024515.4. [Q64LD2-1]
DR   RefSeq; XP_005268113.1; XM_005268056.4.
DR   RefSeq; XP_011535452.1; XM_011537150.2.
DR   AlphaFoldDB; Q64LD2; -.
DR   SMR; Q64LD2; -.
DR   BioGRID; 122671; 63.
DR   IntAct; Q64LD2; 30.
DR   STRING; 9606.ENSP00000334148; -.
DR   GlyGen; Q64LD2; 3 sites, 1 O-linked glycan (3 sites).
DR   iPTMnet; Q64LD2; -.
DR   PhosphoSitePlus; Q64LD2; -.
DR   BioMuta; WDR25; -.
DR   DMDM; 126302614; -.
DR   EPD; Q64LD2; -.
DR   jPOST; Q64LD2; -.
DR   MassIVE; Q64LD2; -.
DR   MaxQB; Q64LD2; -.
DR   PaxDb; 9606-ENSP00000334148; -.
DR   PeptideAtlas; Q64LD2; -.
DR   ProteomicsDB; 65918; -. [Q64LD2-1]
DR   ProteomicsDB; 65919; -. [Q64LD2-2]
DR   Pumba; Q64LD2; -.
DR   Antibodypedia; 174; 67 antibodies from 16 providers.
DR   DNASU; 79446; -.
DR   Ensembl; ENST00000335290.10; ENSP00000334148.6; ENSG00000176473.14. [Q64LD2-1]
DR   Ensembl; ENST00000402312.8; ENSP00000385540.3; ENSG00000176473.14. [Q64LD2-1]
DR   Ensembl; ENST00000542471.2; ENSP00000441903.2; ENSG00000176473.14. [Q64LD2-2]
DR   Ensembl; ENST00000554998.5; ENSP00000450661.1; ENSG00000176473.14. [Q64LD2-1]
DR   GeneID; 79446; -.
DR   KEGG; hsa:79446; -.
DR   MANE-Select; ENST00000402312.8; ENSP00000385540.3; NM_001161476.3; NP_001154948.1.
DR   UCSC; uc001yhn.4; human. [Q64LD2-1]
DR   AGR; HGNC:21064; -.
DR   CTD; 79446; -.
DR   DisGeNET; 79446; -.
DR   GeneCards; WDR25; -.
DR   HGNC; HGNC:21064; WDR25.
DR   HPA; ENSG00000176473; Low tissue specificity.
DR   MIM; 618059; gene.
DR   neXtProt; NX_Q64LD2; -.
DR   OpenTargets; ENSG00000176473; -.
DR   PharmGKB; PA142670607; -.
DR   VEuPathDB; HostDB:ENSG00000176473; -.
DR   eggNOG; KOG0282; Eukaryota.
DR   GeneTree; ENSGT00530000063583; -.
DR   HOGENOM; CLU_042878_0_0_1; -.
DR   InParanoid; Q64LD2; -.
DR   OMA; CGGFHPE; -.
DR   OrthoDB; 2782054at2759; -.
DR   PhylomeDB; Q64LD2; -.
DR   TreeFam; TF329325; -.
DR   PathwayCommons; Q64LD2; -.
DR   SignaLink; Q64LD2; -.
DR   BioGRID-ORCS; 79446; 626 hits in 1155 CRISPR screens.
DR   ChiTaRS; WDR25; human.
DR   GenomeRNAi; 79446; -.
DR   Pharos; Q64LD2; Tdark.
DR   PRO; PR:Q64LD2; -.
DR   Proteomes; UP000005640; Chromosome 14.
DR   RNAct; Q64LD2; Protein.
DR   Bgee; ENSG00000176473; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 107 other cell types or tissues.
DR   ExpressionAtlas; Q64LD2; baseline and differential.
DR   Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR001680; WD40_rpt.
DR   PANTHER; PTHR44566; TRANSDUCIN/WD40 REPEAT-LIKE SUPERFAMILY PROTEIN; 1.
DR   PANTHER; PTHR44566:SF1; WD REPEAT-CONTAINING PROTEIN 25; 1.
DR   Pfam; PF00400; WD40; 3.
DR   SMART; SM00320; WD40; 6.
DR   SUPFAM; SSF50978; WD40 repeat-like; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 2.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
DR   Genevisible; Q64LD2; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..544
FT                   /note="WD repeat-containing protein 25"
FT                   /id="PRO_0000256227"
FT   REPEAT          244..286
FT                   /note="WD 1"
FT   REPEAT          290..329
FT                   /note="WD 2"
FT   REPEAT          330..373
FT                   /note="WD 3"
FT   REPEAT          375..420
FT                   /note="WD 4"
FT   REPEAT          424..463
FT                   /note="WD 5"
FT   REPEAT          469..510
FT                   /note="WD 6"
FT   REPEAT          513..544
FT                   /note="WD 7"
FT   REGION          17..74
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          183..208
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        25..42
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..257
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_021331"
FT   VAR_SEQ         258..274
FT                   /note="LSKSHMLLSTSMDKTFK -> MPALCLVLCWFQGLLGE (in isoform
FT                   2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_021332"
FT   VARIANT         59
FT                   /note="K -> R (in dbSNP:rs2273801)"
FT                   /id="VAR_060042"
FT   VARIANT         88
FT                   /note="W -> R (in dbSNP:rs2181170)"
FT                   /evidence="ECO:0000269|PubMed:14702039,
FT                   ECO:0000269|PubMed:15587985"
FT                   /id="VAR_028888"
FT   VARIANT         119
FT                   /note="T -> M (in dbSNP:rs34331240)"
FT                   /id="VAR_060043"
FT   VARIANT         149
FT                   /note="H -> R (in dbSNP:rs2273800)"
FT                   /evidence="ECO:0000269|PubMed:14702039"
FT                   /id="VAR_028889"
FT   CONFLICT        30
FT                   /note="F -> L (in Ref. 2; BAF84649)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        165
FT                   /note="Q -> R (in Ref. 2; BAF84649)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        462
FT                   /note="S -> I (in Ref. 1; AAQ63174)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   544 AA;  60161 MW;  A4E67A5574F776BD CRC64;
     MTARTLSLMA SLVAYDDSDS EAETEHAGSF NATGQQKDTS GVARPPGQDF ASGTLDVPKA
     GAQPTKHGSC EDPGGYRLPL AQLGRSDWGS CPSQRLQWPG KEPQVTFPIK EPSCSSLWTS
     HVPASHMPLA AARFKQVKLS RNFPKSSFHA QSESETVGKN GSSFQKKKCE DCVVPYTPRR
     LRQRQALSTE TGKGKDVEPQ GPPAGRAPAP LYVGPGVSEF IQPYLNSHYK ETTVPRKVLF
     HLRGHRGPVN TIQWCPVLSK SHMLLSTSMD KTFKVWNAVD SGHCLQTYSL HTEAVRAARW
     APCGRRILSG GFDFALHLTD LETGTQLFSG RSDFRITTLK FHPKDHNIFL CGGFSSEMKA
     WDIRTGKVMR SYKATIQQTL DILFLREGSE FLSSTDASTR DSADRTIIAW DFRTSAKISN
     QIFHERFTCP SLALHPREPV FLAQTNGNYL ALFSTVWPYR MSRRRRYEGH KVEGYSVGCE
     CSPGGDLLVT GSADGRVLMY SFRTASRACT LQGHTQACVG TTYHPVLPSV LATCSWGGDM
     KIWH
//