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Q64L89

- MGST1_BOVIN

UniProt

Q64L89 - MGST1_BOVIN

Protein

Microsomal glutathione S-transferase 1

Gene

MGST1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity By similarity.By similarity

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    Enzyme regulationi

    Can be activated by reagents that attack Cys-50 sulfhydryl, such as N-ethylmaleimide. Activation also occurs via nitration of Tyr-93 by peroxynitrite By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei38 – 381GlutathioneBy similarity
    Sitei50 – 501Activates the enzyme when modifiedBy similarity
    Binding sitei73 – 731GlutathioneBy similarity
    Binding sitei74 – 741GlutathioneBy similarity
    Binding sitei76 – 761GlutathioneBy similarity
    Binding sitei81 – 811GlutathioneBy similarity
    Binding sitei121 – 1211GlutathioneBy similarity

    GO - Molecular functioni

    1. glutathione transferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein homotrimerization Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Microsomal glutathione S-transferase 1 (EC:2.5.1.18)
    Short name:
    Microsomal GST-1
    Gene namesi
    Name:MGST1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB
    4. mitochondrial outer membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 155154Microsomal glutathione S-transferase 1PRO_0000246086Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei42 – 421N6-acetyllysineBy similarity
    Modified residuei55 – 551N6-acetyllysineBy similarity
    Modified residuei60 – 601N6-acetyllysineBy similarity

    Post-translational modificationi

    Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.By similarity

    Keywords - PTMi

    Acetylation, Nitration

    Interactioni

    Subunit structurei

    Homotrimer; The trimer binds only one molecule of glutathione.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ64L89.
    SMRiQ64L89. Positions 10-148.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini3 – 97LumenalBy similarity
    Topological domaini34 – 6229CytoplasmicBy similarityAdd
    BLAST
    Topological domaini97 – 993LumenalBy similarity
    Topological domaini124 – 1285CytoplasmicBy similarity
    Topological domaini149 – 1557LumenalBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei10 – 3324HelicalBy similarityAdd
    BLAST
    Transmembranei63 – 9634HelicalBy similarityAdd
    BLAST
    Transmembranei100 – 12324HelicalBy similarityAdd
    BLAST
    Transmembranei129 – 14820HelicalBy similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MAPEG family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG71159.
    HOGENOMiHOG000231759.
    HOVERGENiHBG052470.
    InParanoidiQ64L89.
    KOiK00799.

    Family and domain databases

    Gene3Di1.20.120.550. 1 hit.
    InterProiIPR023352. MAPEG-like_dom.
    IPR001129. Membr-assoc_MAPEG.
    [Graphical view]
    PfamiPF01124. MAPEG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q64L89-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANLSQLMEN EVFMAFASYT TIVLSKMNFM STATAFYRLT KKVFANPEDC    50
    AGFGKGENAK KYLRTDDRVE RVRRAHLNDL ENIVPFLGIG LLYSLSGPDL 100
    STAILHFRLF VRARIYHTIA YLTPLPQPNR ALAFFIGYGV TLSMAYRLLK 150
    SKLYL 155
    Length:155
    Mass (Da):17,645
    Last modified:October 25, 2004 - v1
    Checksum:i0D762B5FF480BCDC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY334548 mRNA. Translation: AAR00934.1.
    RefSeqiNP_001007816.1. NM_001007815.1.
    UniGeneiBt.107316.

    Genome annotation databases

    GeneIDi493719.
    KEGGibta:493719.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY334548 mRNA. Translation: AAR00934.1 .
    RefSeqi NP_001007816.1. NM_001007815.1.
    UniGenei Bt.107316.

    3D structure databases

    ProteinModelPortali Q64L89.
    SMRi Q64L89. Positions 10-148.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 493719.
    KEGGi bta:493719.

    Organism-specific databases

    CTDi 4257.

    Phylogenomic databases

    eggNOGi NOG71159.
    HOGENOMi HOG000231759.
    HOVERGENi HBG052470.
    InParanoidi Q64L89.
    KOi K00799.

    Miscellaneous databases

    NextBioi 20865464.

    Family and domain databases

    Gene3Di 1.20.120.550. 1 hit.
    InterProi IPR023352. MAPEG-like_dom.
    IPR001129. Membr-assoc_MAPEG.
    [Graphical view ]
    Pfami PF01124. MAPEG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of bovine microsomal glutathione-S-transferase 1."
      Maeda A., Palczewski K.
      Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiMGST1_BOVIN
    AccessioniPrimary (citable) accession number: Q64L89
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: October 25, 2004
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3