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Q64L89 (MGST1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Microsomal glutathione S-transferase 1
Short name=Microsomal GST-1
EC=2.5.1.18
Gene names
Name:MGST1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length155 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity By similarity.

Catalytic activity

RX + glutathione = HX + R-S-glutathione.

Enzyme regulation

Can be activated by reagents that attack Cys-50 sulfhydryl, such as N-ethylmaleimide. Activation also occurs via nitration of Tyr-93 by peroxynitrite By similarity.

Subunit structure

Homotrimer; The trimer binds only one molecule of glutathione By similarity.

Subcellular location

Microsome By similarity. Mitochondrion outer membrane; Peripheral membrane protein By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Post-translational modification

Peroxynitrite induces nitration at Tyr-93 which activates the enzyme By similarity.

Sequence similarities

Belongs to the MAPEG family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 155154Microsomal glutathione S-transferase 1
PRO_0000246086

Regions

Topological domain3 – 97Lumenal By similarity
Transmembrane10 – 3324Helical; By similarity
Topological domain34 – 6229Cytoplasmic By similarity
Transmembrane63 – 9634Helical; By similarity
Topological domain97 – 993Lumenal By similarity
Transmembrane100 – 12324Helical; By similarity
Topological domain124 – 1285Cytoplasmic By similarity
Transmembrane129 – 14820Helical; By similarity
Topological domain149 – 1557Lumenal By similarity

Sites

Binding site381Glutathione By similarity
Binding site731Glutathione By similarity
Binding site741Glutathione By similarity
Binding site761Glutathione By similarity
Binding site811Glutathione By similarity
Binding site1211Glutathione By similarity
Site501Activates the enzyme when modified By similarity

Amino acid modifications

Modified residue421N6-acetyllysine By similarity
Modified residue551N6-acetyllysine By similarity
Modified residue931Nitrated tyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q64L89 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 0D762B5FF480BCDC

FASTA15517,645
        10         20         30         40         50         60 
MANLSQLMEN EVFMAFASYT TIVLSKMNFM STATAFYRLT KKVFANPEDC AGFGKGENAK 

        70         80         90        100        110        120 
KYLRTDDRVE RVRRAHLNDL ENIVPFLGIG LLYSLSGPDL STAILHFRLF VRARIYHTIA 

       130        140        150 
YLTPLPQPNR ALAFFIGYGV TLSMAYRLLK SKLYL

« Hide

References

[1]"Characterization of bovine microsomal glutathione-S-transferase 1."
Maeda A., Palczewski K.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY334548 mRNA. Translation: AAR00934.1.
IPIIPI00705019.
RefSeqNP_001007816.1. NM_001007815.1.
UniGeneBt.107316.

3D structure databases

ProteinModelPortalQ64L89.
SMRQ64L89. Positions 10-148.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID493719.
KEGGbta:493719.

Organism-specific databases

CTD4257.

Phylogenomic databases

eggNOGNOG71159.
HOGENOMHOG000231759.
HOVERGENHBG052470.
InParanoidQ64L89.
KOK00799.
OrthoDBEOG4DJJXQ.

Family and domain databases

Gene3D1.20.120.550. 1 hit.
InterProIPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamPF01124. MAPEG. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20865464.

Entry information

Entry nameMGST1_BOVIN
AccessionPrimary (citable) accession number: Q64L89
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 25, 2004
Last modified: April 3, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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