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Q64L89

- MGST1_BOVIN

UniProt

Q64L89 - MGST1_BOVIN

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Protein

Microsomal glutathione S-transferase 1

Gene

MGST1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity (By similarity).By similarity

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Enzyme regulationi

Can be activated by reagents that attack Cys-50 sulfhydryl, such as N-ethylmaleimide. Activation also occurs via nitration of Tyr-93 by peroxynitrite (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381GlutathioneBy similarity
Sitei50 – 501Activates the enzyme when modifiedBy similarity
Binding sitei73 – 731GlutathioneBy similarity
Binding sitei74 – 741GlutathioneBy similarity
Binding sitei76 – 761GlutathioneBy similarity
Binding sitei81 – 811GlutathioneBy similarity
Binding sitei121 – 1211GlutathioneBy similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein homotrimerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Microsomal glutathione S-transferase 1 (EC:2.5.1.18)
Short name:
Microsomal GST-1
Gene namesi
Name:MGST1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. integral component of membrane Source: UniProtKB
  3. mitochondrial outer membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 155154Microsomal glutathione S-transferase 1PRO_0000246086Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei42 – 421N6-acetyllysineBy similarity
Modified residuei55 – 551N6-acetyllysineBy similarity
Modified residuei60 – 601N6-acetyllysineBy similarity

Post-translational modificationi

Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.By similarity

Keywords - PTMi

Acetylation, Nitration

Interactioni

Subunit structurei

Homotrimer; The trimer binds only one molecule of glutathione.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ64L89.
SMRiQ64L89. Positions 10-148.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini3 – 97LumenalBy similarity
Topological domaini34 – 6229CytoplasmicBy similarityAdd
BLAST
Topological domaini97 – 993LumenalBy similarity
Topological domaini124 – 1285CytoplasmicBy similarity
Topological domaini149 – 1557LumenalBy similarity

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 3324HelicalBy similarityAdd
BLAST
Transmembranei63 – 9634HelicalBy similarityAdd
BLAST
Transmembranei100 – 12324HelicalBy similarityAdd
BLAST
Transmembranei129 – 14820HelicalBy similarityAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG71159.
HOGENOMiHOG000231759.
HOVERGENiHBG052470.
InParanoidiQ64L89.
KOiK00799.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64L89-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANLSQLMEN EVFMAFASYT TIVLSKMNFM STATAFYRLT KKVFANPEDC
60 70 80 90 100
AGFGKGENAK KYLRTDDRVE RVRRAHLNDL ENIVPFLGIG LLYSLSGPDL
110 120 130 140 150
STAILHFRLF VRARIYHTIA YLTPLPQPNR ALAFFIGYGV TLSMAYRLLK

SKLYL
Length:155
Mass (Da):17,645
Last modified:October 25, 2004 - v1
Checksum:i0D762B5FF480BCDC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY334548 mRNA. Translation: AAR00934.1.
RefSeqiNP_001007816.1. NM_001007815.1.
UniGeneiBt.107316.

Genome annotation databases

GeneIDi493719.
KEGGibta:493719.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY334548 mRNA. Translation: AAR00934.1 .
RefSeqi NP_001007816.1. NM_001007815.1.
UniGenei Bt.107316.

3D structure databases

ProteinModelPortali Q64L89.
SMRi Q64L89. Positions 10-148.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 493719.
KEGGi bta:493719.

Organism-specific databases

CTDi 4257.

Phylogenomic databases

eggNOGi NOG71159.
HOGENOMi HOG000231759.
HOVERGENi HBG052470.
InParanoidi Q64L89.
KOi K00799.

Miscellaneous databases

NextBioi 20865464.

Family and domain databases

Gene3Di 1.20.120.550. 1 hit.
InterProi IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view ]
Pfami PF01124. MAPEG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of bovine microsomal glutathione-S-transferase 1."
    Maeda A., Palczewski K.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiMGST1_BOVIN
AccessioniPrimary (citable) accession number: Q64L89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 25, 2004
Last modified: October 29, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3