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Q64L89

- MGST1_BOVIN

UniProt

Q64L89 - MGST1_BOVIN

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Protein
Microsomal glutathione S-transferase 1
Gene
MGST1
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity By similarity.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Enzyme regulationi

Can be activated by reagents that attack Cys-50 sulfhydryl, such as N-ethylmaleimide. Activation also occurs via nitration of Tyr-93 by peroxynitrite By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381Glutathione By similarity
Sitei50 – 501Activates the enzyme when modified By similarity
Binding sitei73 – 731Glutathione By similarity
Binding sitei74 – 741Glutathione By similarity
Binding sitei76 – 761Glutathione By similarity
Binding sitei81 – 811Glutathione By similarity
Binding sitei121 – 1211Glutathione By similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein homotrimerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Microsomal glutathione S-transferase 1 (EC:2.5.1.18)
Short name:
Microsomal GST-1
Gene namesi
Name:MGST1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini3 – 97Lumenal By similarity
Transmembranei10 – 3324Helical; By similarity
Add
BLAST
Topological domaini34 – 6229Cytoplasmic By similarity
Add
BLAST
Transmembranei63 – 9634Helical; By similarity
Add
BLAST
Topological domaini97 – 993Lumenal By similarity
Transmembranei100 – 12324Helical; By similarity
Add
BLAST
Topological domaini124 – 1285Cytoplasmic By similarity
Transmembranei129 – 14820Helical; By similarity
Add
BLAST
Topological domaini149 – 1557Lumenal By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB
  4. mitochondrial outer membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 155154Microsomal glutathione S-transferase 1
PRO_0000246086Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei42 – 421N6-acetyllysine By similarity
Modified residuei55 – 551N6-acetyllysine By similarity
Modified residuei60 – 601N6-acetyllysine By similarity

Post-translational modificationi

Peroxynitrite induces nitration at Tyr-93 which activates the enzyme By similarity.

Keywords - PTMi

Acetylation, Nitration

Interactioni

Subunit structurei

Homotrimer; The trimer binds only one molecule of glutathione By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ64L89.
SMRiQ64L89. Positions 10-148.

Family & Domainsi

Sequence similaritiesi

Belongs to the MAPEG family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG71159.
HOGENOMiHOG000231759.
HOVERGENiHBG052470.
InParanoidiQ64L89.
KOiK00799.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64L89-1 [UniParc]FASTAAdd to Basket

« Hide

MANLSQLMEN EVFMAFASYT TIVLSKMNFM STATAFYRLT KKVFANPEDC    50
AGFGKGENAK KYLRTDDRVE RVRRAHLNDL ENIVPFLGIG LLYSLSGPDL 100
STAILHFRLF VRARIYHTIA YLTPLPQPNR ALAFFIGYGV TLSMAYRLLK 150
SKLYL 155
Length:155
Mass (Da):17,645
Last modified:October 25, 2004 - v1
Checksum:i0D762B5FF480BCDC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY334548 mRNA. Translation: AAR00934.1.
RefSeqiNP_001007816.1. NM_001007815.1.
UniGeneiBt.107316.

Genome annotation databases

GeneIDi493719.
KEGGibta:493719.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY334548 mRNA. Translation: AAR00934.1 .
RefSeqi NP_001007816.1. NM_001007815.1.
UniGenei Bt.107316.

3D structure databases

ProteinModelPortali Q64L89.
SMRi Q64L89. Positions 10-148.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 493719.
KEGGi bta:493719.

Organism-specific databases

CTDi 4257.

Phylogenomic databases

eggNOGi NOG71159.
HOGENOMi HOG000231759.
HOVERGENi HBG052470.
InParanoidi Q64L89.
KOi K00799.

Miscellaneous databases

NextBioi 20865464.

Family and domain databases

Gene3Di 1.20.120.550. 1 hit.
InterProi IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view ]
Pfami PF01124. MAPEG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of bovine microsomal glutathione-S-transferase 1."
    Maeda A., Palczewski K.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiMGST1_BOVIN
AccessioniPrimary (citable) accession number: Q64L89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 25, 2004
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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