Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein-L-isoaspartate O-methyltransferase 2

Gene

PIMT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei144 – 1441By similarity

GO - Molecular functioni

  • protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Source: TAIR

GO - Biological processi

  • protein repair Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT5G50240-MONOMER.
ARA:GQT-1084-MONOMER.
ARA:GQT-1085-MONOMER.
BRENDAi2.1.1.77. 399.
ReactomeiR-ATH-5676934. Protein repair.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-L-isoaspartate O-methyltransferase 2 (EC:2.1.1.77)
Short name:
AtPIMT2
Gene namesi
Name:PIMT2
Ordered Locus Names:At5g50240
ORF Names:K6A12.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G50240.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi23 – 242KK → RR: Loss of nuclear targeting. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Protein-L-isoaspartate O-methyltransferase 2PRO_0000428878Add
BLAST

Proteomic databases

PaxDbiQ64J17.
PRIDEiQ64J17.

Expressioni

Tissue specificityi

Expressed in rosette leaves, stems, cauline leaves, flowers and developing seeds.1 Publication

Inductioni

By abscisic acid (ABA), drought and salt stress.1 Publication

Gene expression databases

ExpressionAtlasiQ64J17. baseline and differential.
GenevisibleiQ64J17. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G50240.1.

Structurei

3D structure databases

ProteinModelPortaliQ64J17.
SMRiQ64J17. Positions 95-305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi23 – 286Nuclear localization signalCurated

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1661. Eukaryota.
COG2518. LUCA.
HOGENOMiHOG000257189.
InParanoidiQ64J17.
KOiK00573.
OMAiLYCAPRR.
OrthoDBiEOG09360H12.
PhylomeDBiQ64J17.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q64J17-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNTNTQTEQQ IIREETRIDK IIKKRKKKMR AQVLLCPTIT AYGRLYCAPR
60 70 80 90 100
RLHRYNSSSS SSQFLNLNLS RFSGALFFHM EQFQSGTGSS GKRGMVENLK
110 120 130 140 150
RYGVISSKRV AQVMEALDRG LFVPVGSSAY VDTPVPIGYN ATISAPHMHA
160 170 180 190 200
TCLQLLEDKL HPGMRALDVG SGTGYLTGCF ALMVGAEGRV VGVDHIPELV
210 220 230 240 250
DMSIKNIEKS VAASFLKKGS LSLHVGDGRK GWQEFAPYDA IHVGAAASEI
260 270 280 290 300
PQPLLDQLKP GGRMVIPLGT YFQELKVIDK NEDGSIKVHT ETSVRYVPLT

SRVEQLGGF
Length:309
Mass (Da):33,986
Last modified:October 25, 2004 - v1
Checksum:iE4220BAE9B39656F
GO
Isoform 2 (identifier: Q64J17-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     82-84: Missing.

Note: May be due to a competing acceptor splice site.
Show »
Length:306
Mass (Da):33,583
Checksum:iEFE17CE9CAA779E5
GO
Isoform 3 (identifier: Q64J17-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-84: MNTNTQTEQQ...ALFFHMEQFQ → ME

Show »
Length:227
Mass (Da):24,294
Checksum:iDE77F8890179F955
GO

Sequence cautioni

The sequence BAB09395 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8484MNTNT…MEQFQ → ME in isoform 3. 1 PublicationVSP_054319Add
BLAST
Alternative sequencei82 – 843Missing in isoform 2. CuratedVSP_054320

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY496702 Genomic DNA. Translation: AAR97903.1.
AY496702 Genomic DNA. Translation: AAR97904.1.
AY496702 Genomic DNA. Translation: ACL14906.1.
AB024031 Genomic DNA. Translation: BAB09395.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95915.1.
CP002688 Genomic DNA. Translation: AED95916.1.
AK118104 mRNA. Translation: BAC42732.1.
RefSeqiNP_001078740.1. NM_001085271.1. [Q64J17-2]
NP_199835.2. NM_124403.2. [Q64J17-1]
UniGeneiAt.46085.
At.7917.

Genome annotation databases

EnsemblPlantsiAT5G50240.1; AT5G50240.1; AT5G50240. [Q64J17-1]
AT5G50240.2; AT5G50240.2; AT5G50240. [Q64J17-2]
GeneIDi835089.
KEGGiath:AT5G50240.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY496702 Genomic DNA. Translation: AAR97903.1.
AY496702 Genomic DNA. Translation: AAR97904.1.
AY496702 Genomic DNA. Translation: ACL14906.1.
AB024031 Genomic DNA. Translation: BAB09395.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95915.1.
CP002688 Genomic DNA. Translation: AED95916.1.
AK118104 mRNA. Translation: BAC42732.1.
RefSeqiNP_001078740.1. NM_001085271.1. [Q64J17-2]
NP_199835.2. NM_124403.2. [Q64J17-1]
UniGeneiAt.46085.
At.7917.

3D structure databases

ProteinModelPortaliQ64J17.
SMRiQ64J17. Positions 95-305.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G50240.1.

Proteomic databases

PaxDbiQ64J17.
PRIDEiQ64J17.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G50240.1; AT5G50240.1; AT5G50240. [Q64J17-1]
AT5G50240.2; AT5G50240.2; AT5G50240. [Q64J17-2]
GeneIDi835089.
KEGGiath:AT5G50240.

Organism-specific databases

TAIRiAT5G50240.

Phylogenomic databases

eggNOGiKOG1661. Eukaryota.
COG2518. LUCA.
HOGENOMiHOG000257189.
InParanoidiQ64J17.
KOiK00573.
OMAiLYCAPRR.
OrthoDBiEOG09360H12.
PhylomeDBiQ64J17.

Enzyme and pathway databases

BioCyciARA:AT5G50240-MONOMER.
ARA:GQT-1084-MONOMER.
ARA:GQT-1085-MONOMER.
BRENDAi2.1.1.77. 399.
ReactomeiR-ATH-5676934. Protein repair.

Miscellaneous databases

PROiQ64J17.

Gene expression databases

ExpressionAtlasiQ64J17. baseline and differential.
GenevisibleiQ64J17. AT.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIMT2_ARATH
AccessioniPrimary (citable) accession number: Q64J17
Secondary accession number(s): Q64J16, Q8GXQ4, Q9FGS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 14, 2014
Last sequence update: October 25, 2004
Last modified: September 7, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.