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Q647G9 (CONG_ARAHY) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Conglutin
Alternative name(s):
Allergen=Ara h 6
OrganismArachis hypogaea (Peanut)
Taxonomic identifier3818 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Dalbergieae › Arachis

Protein attributes

Sequence length145 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Tissue specificity

Expressed in seeds. Not expressed in roots, pegs (budding ovaries) or leaves. Ref.2

Developmental stage

Expressed in the later, cell expansion, stages of seed development. Ref.2

Induction

Repressed by water stress. Ref.4

Allergenic properties

Causes an allergic reaction in human. Binds to IgE. Ref.3 Ref.6

Sequence similarities

Belongs to the 2S seed storage albumins family.

Mass spectrometry

Molecular mass is 17479 Da from positions 22 - 145. Determined by ESI. Ref.4

Ontologies

Keywords
   DiseaseAllergen
   DomainSignal
   LigandIgE-binding protein
   Molecular functionSeed storage protein
Storage protein
   PTMDisulfide bond
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Molecular functionIgE binding

Inferred from electronic annotation. Source: UniProtKB-KW

nutrient reservoir activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 145124Conglutin
PRO_0000272966

Amino acid modifications

Disulfide bond35 ↔ 92 Ref.6 Ref.6
Disulfide bond47 ↔ 79 Ref.6 Ref.6
Disulfide bond80 ↔ 128 Ref.6 Ref.6
Disulfide bond94 ↔ 136 Ref.6 Ref.6
Disulfide bond105 ↔ 145 Ref.6 Ref.6

Experimental info

Sequence conflict411R → G in AAD56337. Ref.3
Sequence conflict66 – 683DIR → NFG in AAD56337. Ref.3
Sequence conflict84 – 852NE → DQ in AAW56068. Ref.1
Sequence conflict84 – 852NE → DQ in AAL37561. Ref.2
Sequence conflict901Q → E in AAW56068. Ref.1
Sequence conflict901Q → E in AAL37561. Ref.2
Sequence conflict911R → G in AAW34231. Ref.5
Sequence conflict1071R → G in AAD56337. Ref.3
Sequence conflict1161Q → H in AAD56337. Ref.3
Sequence conflict1231N → S in AAW34231. Ref.5
Sequence conflict1311R → G in AAD56337. Ref.3

Secondary structure

1 .................. 145
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q647G9 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: FA3D6FC41CB6267D

FASTA14516,920
        10         20         30         40         50         60 
MAKSTILVAL LALVLVAHAS AMRRERGRQG DSSSCERQVD RVNLKPCEQH IMQRIMGEQE 

        70         80         90        100        110        120 
QYDSYDIRST RSSDQQQRCC DELNEMENTQ RCMCEALQQI MENQCDRLQD RQMVQQFKRE 

       130        140 
LMNLPQQCNF RAPQRCDLDV SGGRC

« Hide

References

[1]"Isolation of peanut genes encoding arachins and conglutins by expressed sequence tags."
Yan Y.-S., Lin X.-D., Zhang Y.-S., Wang L., Wu K., Huang S.-Z.
Plant Sci. 169:439-445(2005) [Agricola: IND43739496]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Shanyou 523.
Tissue: Cotyledon.
[2]"Seed-specific, developmentally regulated genes of peanut."
Paik-Ro O.G., Seib J.C., Smith R.L.
Theor. Appl. Genet. 104:236-240(2002) [PubMed: 12582692] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-145, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: cv. FL435.
Tissue: Seed.
[3]"Selective cloning of peanut allergens, including profilin and 2S albumins, by phage display technology."
Kleber-Janke T., Crameri R., Appenzeller U., Schlaak M., Becker W.-M.
Int. Arch. Allergy Immunol. 119:265-274(1999) [PubMed: 10474031] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-145, ALLERGEN.
Strain: cv. Virginia.
Tissue: Seed.
[4]"Suppression of seed storage proteins upon water stress in Arachis hypogea var. M-13 seeds."
Katam R., Vasanthaiah H.K.N., Basha S.M., McClung S.
Submitted (MAR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 22-145, REPRESSION BY WATER STRESS, MASS SPECTROMETRY.
Strain: cv. M13.
Tissue: Seed.
[5]"Epitope mapping of Ara h 6."
Schocker F., Suhr M., Becker W.-M.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-145.
[6]"Structure and stability of 2S albumin-type peanut allergens: implications for the severity of peanut allergic reactions."
Lehmann K., Schweimer K., Reese G., Randow S., Suhr M., Becker W.-M., Vieths S., Roesch P.
Biochem. J. 395:463-472(2006) [PubMed: 16372900] [Abstract]
Cited for: STRUCTURE BY NMR OF 22-145, PARTIAL PROTEIN SEQUENCE, ALLERGEN, DISULFIDE BONDS.
Tissue: Seed.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY722690 mRNA. Translation: AAU21495.1.
AY848699 mRNA. Translation: AAW56068.1.
AY849314 mRNA. Translation: AAW32558.1.
AF366561 mRNA. Translation: AAL37561.1.
AF092846 mRNA. Translation: AAD56337.1.
AY871100 Genomic DNA. Translation: AAW34231.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1W2QNMR-A22-145[»]
ProteinModelPortalQ647G9.
SMRQ647G9. Positions 22-145.
ModBaseSearch...

Protein family/group databases

Allergome3098. Ara h 6.0101.
55. Ara h 6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR016140. Bifunc_inhib/LTP/seed_store.
IPR003612. LTP/seed_store/tryp_amyl_inhib.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]
Gene3DG3DSA:1.10.120.10. Trypsin/amylase_inhib. 1 hit.
PfamPF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]
SMARTSM00499. AAI. 1 hit.
[Graphical view]
SUPFAMSSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCONG_ARAHY
AccessionPrimary (citable) accession number: Q647G9
Secondary accession number(s): Q5I1E9 expand/collapse secondary AC list , Q5I6T1, Q8W251, Q9SQG5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 25, 2004
Last modified: September 21, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Allergens

Nomenclature of allergens and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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