Reviewed, UniProtKB/Swiss-Prot Q647G9 (CONG_ARAHY)

Names and origin

Protein names	Recommended name:     Conglutin Alternative name(s):     Allergen=Ara h 6
Organism	Arachis hypogaea (Peanut)
Taxonomic identifier	3818 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Dalbergieae › Arachis

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Tissue specificity	Expressed in seeds. Not expressed in roots, pegs (budding ovaries) or leaves. Ref.2
Developmental stage	Expressed in the later, cell expansion, stages of seed development. Ref.2
Induction	Repressed by water stress. Ref.4
Allergenic properties	Causes an allergic reaction in human. Binds to IgE. Ref.3
Sequence similarities	Belongs to the 2S seed storage albumins family.
Mass spectrometry	Molecular mass is 17479 Da from positions 22 - 145. Determined by ESI. Ref.4

Ontologies

Keywords
Disease	Allergen
Domain	Signal
Ligand	IgE-binding protein
Molecular function	Seed storage protein Storage protein
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	IgE binding Inferred from electronic annotation. Source: UniProtKB-KW nutrient reservoir activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Potential

Chain

22 – 145

124

Conglutin

PRO_0000272966

Amino acid modifications

Disulfide bond

35 ↔ 92

Ref.6

Disulfide bond

47 ↔ 79

Ref.6

Disulfide bond

80 ↔ 128

Ref.6

Disulfide bond

94 ↔ 136

Ref.6

Disulfide bond

105 ↔ 145

Ref.6

Experimental info

Sequence conflict

41

1

R → G in AAD56337. Ref.3

Sequence conflict

66 – 68

3

DIR → NFG in AAD56337. Ref.3

Sequence conflict

84 – 85

2

NE → DQ in AAW56068. Ref.1

Sequence conflict

84 – 85

2

NE → DQ in AAL37561. Ref.2

Sequence conflict

90

1

Q → E in AAW56068. Ref.1

Sequence conflict

90

1

Q → E in AAL37561. Ref.2

Sequence conflict

91

1

R → G in AAW34231. Ref.5

Sequence conflict

107

1

R → G in AAD56337. Ref.3

Sequence conflict

116

1

Q → H in AAD56337. Ref.3

Sequence conflict

123

1

N → S in AAW34231. Ref.5

Sequence conflict

131

1

R → G in AAD56337. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

145

Helix Strand Turn

Details...

Helix

35 – 39

5

Helix

45 – 52

8

Helix

54 – 56

3

Beta strand

57 – 61

5

Helix

72 – 85

14

Helix

93 – 103

11

Turn

106 – 110

5

Helix

114 – 128

15

Beta strand

139 – 141

3

Sequences

	Sequence		Length	Mass (Da)
	Q647G9-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: FA3D6FC41CB6267D	FASTA	145	16,920
	10 20 30 40 50 60 MAKSTILVAL LALVLVAHAS AMRRERGRQG DSSSCERQVD RVNLKPCEQH IMQRIMGEQE 70 80 90 100 110 120 QYDSYDIRST RSSDQQQRCC DELNEMENTQ RCMCEALQQI MENQCDRLQD RQMVQQFKRE 130 140 LMNLPQQCNF RAPQRCDLDV SGGRC

References

[1]	"Isolation of peanut genes encoding arachins and conglutins by expressed sequence tags." Yan Y.-S., Lin X.-D., Zhang Y.-S., Wang L., Wu K., Huang S.-Z. Plant Sci. 169:439-445(2005) [Agricola: IND43739496] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Shanyou 523. Tissue: Cotyledon.
[2]	"Seed-specific, developmentally regulated genes of peanut." Paik-Ro O.G., Seib J.C., Smith R.L. Theor. Appl. Genet. 104:236-240(2002) [PubMed: 12582692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-145, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: cv. FL435. Tissue: Seed.
[3]	"Selective cloning of peanut allergens, including profilin and 2S albumins, by phage display technology." Kleber-Janke T., Crameri R., Appenzeller U., Schlaak M., Becker W.-M. Int. Arch. Allergy Immunol. 119:265-274(1999) [PubMed: 10474031] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-145, ALLERGENICITY. Strain: cv. Virginia. Tissue: Seed.
[4]	"Suppression of seed storage proteins upon water stress in Arachis hypogea var. M-13 seeds." Katam R., Vasanthaiah H.K.N., Basha S.M., McClung S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 22-145, REPRESSION BY WATER STRESS, MASS SPECTROMETRY. Strain: cv. M13. Tissue: Seed.
[5]	"Epitope mapping of Ara h 6." Schocker F., Suhr M., Becker W.-M. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-145.
[6]	"Allergen Arah6 from peanut (Arachis hypogaea)." Lehmann K., Schweimer K., Vieths S., Roesch P. Submitted (JUL-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 22-145. Tissue: Seed.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AY722690 mRNA. Translation: AAU21495.1.
AY848699 mRNA. Translation: AAW56068.1.
AY849314 mRNA. Translation: AAW32558.1.
AF366561 mRNA. Translation: AAL37561.1.
AF092846 mRNA. Translation: AAD56337.1.
AY871100 Genomic DNA. Translation: AAW34231.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1W2Q	NMR	-	A	22-145	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR016140. Bifunc_inhib/LTP/seed_store.
IPR003612. LTP/seed_store/tryp_amyl_inhib.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]

Gene3D

G3DSA:1.10.120.10. Trypsin/amylase_inhib. 1 hit.

Pfam

PF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]

SMART

SM00499. AAI. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CONG_ARAHY

Accession

Primary (citable) accession number: Q647G9
Secondary accession number(s): Q5I1E9 , Q5I6T1, Q8W251, Q9SQG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	October 25, 2004
Last modified:	November 24, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)