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Reviewed, UniProtKB/Swiss-Prot Q64737 (PUR2_MOUSE)

Last modified October 13, 2009. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
    1- Recommended name:
            Phosphoribosylamine--glycine ligase
              EC=6.3.4.13
        Alternative name(s):
            Glycinamide ribonucleotide synthetase
              Short name=GARS
            Phosphoribosylglycinamide synthetase
    2- Recommended name:
            Phosphoribosylformylglycinamidine cyclo-ligase
              EC=6.3.3.1
        Alternative name(s):
            Phosphoribosyl-aminoimidazole synthetase
            AIR synthase
              Short name=AIRS
    3- Recommended name:
            Phosphoribosylglycinamide formyltransferase
              EC=2.1.2.2
        Alternative name(s):
            5'-phosphoribosylglycinamide transformylase
            GAR transformylase
              Short name=GART
Gene names
Name: Gart
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length1010 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.

Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.

Tissue specificity

Detected in liver, kidney and brain. Ref.2

Sequence similarities

In the N-terminal section; belongs to the GARS family.

In the central section; belongs to the AIR synthase family.

In the C-terminal section; belongs to the GART family.

Contains 1 ATP-grasp domain.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q64737-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q64737-2)

The sequence of this isoform differs from the canonical sequence as follows:
     434-1010: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10101010Trifunctional purine biosynthetic protein adenosine-3
PRO_0000074938

Regions

Domain111 – 318208ATP-grasp
Nucleotide binding137 – 19963ATP By similarity
Region434 – 807374AIRS
Region808 – 1010203GART
Region818 – 82035'-phosphoribosylglycinamide binding By similarity
Region896 – 899410-formyltetrahydrofolate binding By similarity
Region947 – 951510-formyltetrahydrofolate binding By similarity
Region977 – 98045'-phosphoribosylglycinamide binding By similarity

Sites

Active site9151Proton donor By similarity
Metal binding2881Manganese By similarity
Metal binding2901Manganese By similarity
Binding site871110-formyltetrahydrofolate By similarity
Binding site913110-formyltetrahydrofolate By similarity
Site9511Raises pKa of active site His By similarity

Amino acid modifications

Modified residue3481Phosphotyrosine By similarity
Modified residue3501N6-acetyllysine By similarity

Natural variations

Alternative sequence434 – 1010577Missing in isoform Short.
VSP_005518

Experimental info

Sequence conflict411G → C in AAH70465. Ref.3
Sequence conflict3181D → G in AAC53250. Ref.1
Sequence conflict3181D → G in AAC53251. Ref.1
Sequence conflict5631A → G in AAH70465. Ref.3
Sequence conflict6901I → T in AAA19013. Ref.2
Sequence conflict8681S → Y in AAH70465. Ref.3

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Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified July 19, 2004. Version 2.
Checksum: 40D9E6B7385E9384

FASTA1,010107,395
        10         20         30         40         50         60 
MAARVLVIGS GGREHTLAWK LAQSPQVKQV LVAPGNAGTA GAGKISNAAV SVNDHSALAQ 

        70         80         90        100        110        120 
FCKDEKIELV VVGPEAPLAA GIVGDLTSAG VRCFGPTAQA AQLESSKKFA KEFMDRHEIP 

       130        140        150        160        170        180 
TAQWRAFTNP EDACSFITSA NFPALVVKAS GLAAGKGVIV AKSQAEACRA VQEIMQEKSF 

       190        200        210        220        230        240 
GAAGETVVVE EFLEGEEVSC LCFTDGKTVA EMPPAQDHKR LLDGDEGPNT GGMGAYCPAP 

       250        260        270        280        290        300 
QVSKDLLVKI KNTILQRAVD GMQQEGAPYT GILYAGIMLT KDGPKVLEFN CRFGDPECQV 

       310        320        330        340        350        360 
ILPLLKSDLY EVMQSTLDGL LSASLPVWLE NHSAVTVVMA SKGYPGAYTK GVEITGFPEA 

       370        380        390        400        410        420 
QALGLQVFHA GTALKDGKVV TSGGRVLTVT AVQENLMSAL AEARKGLAAL KFEGAIYRKD 

       430        440        450        460        470        480 
IGFRAVAFLQ RPRGLTYKDS GVDIAAGNML VKKIQPLAKA TSRPGCSVDL GGFAGLFDLK 

       490        500        510        520        530        540 
AAGFKDPLLA SGTDGVGTKL KIAQLCNKHD SIGQDLVAMC VNDILAQGAE PLFFLDYFSC 

       550        560        570        580        590        600 
GKLDLSTTEA VIAGIAAACQ QAACALLGGE TAEMPNMYPP GEYDLAGFAV GAMERHQKLP 

       610        620        630        640        650        660 
QLERITEGDA VIGVASSGLH SNGFSLVRKI VERSSLQYSS PAPGGCGDQT LGDLLLTPTR 

       670        680        690        700        710        720 
IYSHSLLPII RSGRVKAFAH ITGGGLLENI PRVLPQKFGV DLDASTWRVP KVFSWLQQEG 

       730        740        750        760        770        780 
ELSEEEMART FNCGIGAALV VSKDQAEQVL HDVRRRQEEA WVIGSVVACP EDSPRVRVKN 

       790        800        810        820        830        840 
LIETIQTNGS LVANGFLKSN FPVQQKKARV AVLISGTGSN LQALIDSTRD PKSSSHIVLV 

       850        860        870        880        890        900 
ISNKAAVAGL DRAERAGIPT RVINHKLSKN RVEFDNAVDH VLEEFSVDIV CLAGFMRILS 

       910        920        930        940        950        960 
GPFVRKWDGK MLNIHPSLLP SFKGSNAHEQ VLEAGVTITG CTVHFVAEDV DAGQIILQEA 

       970        980        990       1000       1010 
VPVRRGDTVA TLSERVKVAE HKIFPAALQL VASGAVQLRE DGKIHWAKEQ

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Isoform Short.

Checksum: 18E7942476DD75FA
Show »

FASTA43345,651

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References

« Hide 'large scale' references
[1]"Mouse cDNAs encoding a trifunctional protein of de novo purine synthesis and a related single-domain glycinamide ribonucleotide synthetase."
Kan J.L., Jannatipour M., Taylor S.M., Moran R.G.
Gene 137:195-202(1993) [PubMed: 8299947] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
Strain: C57BL/6 X CBA.
Tissue: Spleen.
[2]"Analysis of a mouse gene encoding three steps of purine synthesis reveals use of an intronic polyadenylation signal without alternative exon usage."
Kan J.L., Moran R.G.
J. Biol. Chem. 270:1823-1832(1995) [PubMed: 7829519] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Strain: C57BL/6.
Tissue: Eye.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U01023 mRNA. Translation: AAA19012.1.
U01024 mRNA. Translation: AAA19013.1.
U20886 expand/collapse EMBL AC list , U20875, U20876, U20877, U20879, U20880, U20881, U20882, U20883 Genomic DNA. Translation: AAC53250.1.
U20892 expand/collapse EMBL AC list , U20875, U20876, U20877, U20879, U20880, U20881, U20882, U20883, U20886, U20884, U20887, U20885, U20889, U20890, U20891 Genomic DNA. Translation: AAC53251.1.
BC070465 mRNA. Translation: AAH70465.1.
IPIIPI00230612.
IPI00323644.
PIRI67805.
RefSeqNP_034386.2.
UniGeneMm.4505

3D structure databases

HSSPHSSP built from PDB template 1NJS based on UniProtKB P22102.
SMRQ64737. Positions 808-1006.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ64737.

PTM databases

PhosphoSiteQ64737.

Proteomic databases

PRIDEQ64737.

Genome annotation databases

EnsemblENSMUST00000023684; ENSMUSP00000023684; ENSMUSG00000022962; Mus musculus. [Genome view]
GeneID14450.
KEGGmmu:14450.

Organism-specific databases

MGIMGI:95654. Gart.

Phylogenomic databases

HOGENOMQ64737.
HOVERGENQ64737.

Enzyme and pathway databases

BRENDA2.1.2.2. 244.
6.3.3.1. 244.
6.3.4.13. 244.

Gene expression databases

ArrayExpressQ64737.
BgeeQ64737.
CleanExMM_GART.
GenevestigatorQ64737.
GermOnlineENSMUSG00000022962. Mus musculus.

Family and domain databases

InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR013817. Pre-ATP_grasp.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N-dom.
IPR004733. PurM_cligase.
IPR004607. PurN_trans.
[Graphical view]
Gene3DG3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit.
G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
TIGR00639. PurN. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

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Entry information

Entry namePUR2_MOUSE
AccessionPrimary (citable) accession number: Q64737
Secondary accession number(s): Q6NS48
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 19, 2004
Last modified: October 13, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents